5y03 - Revision history

OCA at 07:02, 14 October 2020

OCA — Wed, 14 Oct 2020 07:02:07 GMT

←Older revision		Revision as of 07:02, 14 October 2020
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	==Galectin-13/Placental Protein 13 variant R53H crystal structure==		==Galectin-13/Placental Protein 13 variant R53H crystal structure==
-	<StructureSection load='5y03' size='340' side='right' caption='[[5y03]], [[Resolution\|resolution]] 2.12Å' scene=''>	+	<StructureSection load='5y03' size='340' side='right'caption='[[5y03]], [[Resolution\|resolution]] 2.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y03]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y03 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Y03 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y03]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y03 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Y03 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5y03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y03 OCA], [http://pdbe.org/5y03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y03 RCSB], [http://www.ebi.ac.uk/pdbsum/5y03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y03 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5y03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y03 OCA], [http://pdbe.org/5y03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y03 RCSB], [http://www.ebi.ac.uk/pdbsum/5y03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y03 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5y03" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5y03" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Su, J]]		[[Category: Su, J]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
	[[Category: Galectin-13/placental protein 13]]		[[Category: Galectin-13/placental protein 13]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 05:54, 31 January 2018

OCA — Wed, 31 Jan 2018 05:54:19 GMT

←Older revision		Revision as of 05:54, 31 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5y03 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Galectin-13/Placental Protein 13 variant R53H crystal structure==
		+	<StructureSection load='5y03' size='340' side='right' caption='[[5y03]], [[Resolution\|resolution]] 2.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5y03]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y03 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y03 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y03 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y03 OCA], [http://pdbe.org/5y03 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y03 RCSB], [http://www.ebi.ac.uk/pdbsum/5y03 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y03 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PP13_HUMAN PP13_HUMAN]] Binds beta-galactoside and lactose. Strong inducer of T-cell apoptosis.<ref>PMID:10527825</ref> <ref>PMID:19497882</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During pregnancy, placental protein-13 (galectin-13) is highly expressed in the placenta and fetal tissue, and less so in maternal serum that is related to pre-eclampsia. To understand galectin-13 function at the molecular level, we solved its crystal structure and discovered that its dimer is stabilized by two disulfide bridges between Cys136 and Cys138 and six hydrogen bonds involving Val135, Val137, and Gln139. Native PAGE and gel filtration demonstrate that this is not a crystallization artifact because dimers also form in solution. Our biochemical studies indicate that galectin-13 ligand binding specificity is different from that of other galectins in that it does not bind beta-galactosides. This is partly explained by the presence of Arg53 rather than His53 at the bottom of the carbohydrate binding site in a position that is crucial for interactions with beta-galactosides. Mutating Arg53 to histidine does not re-establish normal beta-galactoside binding, but rather traps cryoprotectant glycerol molecules within the ligand binding site in crystals of the R53H mutant. Moreover, unlike most other galectins, we also found that GFP-tagged galectin-13 is localized within the nucleus of HeLa and 293 T cells. Overall, galectin-13 appears to be a new type of prototype galectin with distinct properties.

-	~~Authors: Wang~~, Y., Su, J.	+	Galectin-13, a different prototype galectin, does not bind beta-galacto-sides and forms dimers via intermolecular disulfide bridges between Cys-136 and Cys-138.,Su J, Wang Y, Si Y, Gao J, Song C, Cui L, Wu R, Tai G, Zhou Y Sci Rep. 2018 Jan 17;8(1):980. doi: 10.1038/s41598-018-19465-0. PMID:29343868<ref>PMID:29343868</ref>

-	~~Description: Galectin-13~~/~~Placental Protein 13 variant R53H crystal structure~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
		+	<div class="pdbe-citations 5y03" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Su, J]]		[[Category: Su, J]]
	[[Category: Wang, Y]]		[[Category: Wang, Y]]
		+	[[Category: Galectin-13/placental protein 13]]
		+	[[Category: Sugar binding protein]]

OCA at 07:02, 10 January 2018

OCA — Wed, 10 Jan 2018 07:02:01 GMT

←Older revision		Revision as of 07:02, 10 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5y03 is ON HOLD	+	The entry 5y03 is ON HOLD until Paper Publication

	Authors: Wang, Y., Su, J.		Authors: Wang, Y., Su, J.
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	Description: Galectin-13/Placental Protein 13 variant R53H crystal structure		Description: Galectin-13/Placental Protein 13 variant R53H crystal structure
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wang, Y]]
	[[Category: Su, J]]		[[Category: Su, J]]
		+	[[Category: Wang, Y]]

OCA: Protected "5y03" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 15:59:16 GMT

Protected "5y03" [edit=sysop:move=sysop]

←Older revision

Revision as of 15:59, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5y03 is ON HOLD Authors: Wang, Y., Su, J. Description: Galectin-13/Placental Protein 13 variant R53H crystal structure [[Category: Unreleased Struc...

OCA — Wed, 27 Sep 2017 15:59:14 GMT

New page: '''Unreleased structure''' The entry 5y03 is ON HOLD Authors: Wang, Y., Su, J. Description: Galectin-13/Placental Protein 13 variant R53H crystal structure [[Category: Unreleased Struc...

New page

'''Unreleased structure'''

The entry 5y03 is ON HOLD

Authors: Wang, Y., Su, J.

Description: Galectin-13/Placental Protein 13 variant R53H crystal structure
[[Category: Unreleased Structures]]
[[Category: Wang, Y]]
[[Category: Su, J]]