5y31 - Revision history

OCA at 12:34, 7 November 2018

OCA — Wed, 07 Nov 2018 12:34:18 GMT

←Older revision		Revision as of 12:34, 7 November 2018
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-	==Crystal structure of human LGI1 ~~and human~~ ADAM22 complex==	+	==Crystal structure of human LGI1-ADAM22 complex==
	<StructureSection load='5y31' size='340' side='right' caption='[[5y31]], [[Resolution\|resolution]] 7.12Å' scene=''>		<StructureSection load='5y31' size='340' side='right' caption='[[5y31]], [[Resolution\|resolution]] 7.12Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y31]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y31 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y31]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y31 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ADAM22, MDC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), LGI1, EPT, UNQ775/PRO1569 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y31 OCA], [http://pdbe.org/5y31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y31 RCSB], [http://www.ebi.ac.uk/pdbsum/5y31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y31 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y31 OCA], [http://pdbe.org/5y31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y31 RCSB], [http://www.ebi.ac.uk/pdbsum/5y31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y31 ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Fukai, S]]		[[Category: Fukai, S]]
	[[Category: Yamagata, A]]		[[Category: Yamagata, A]]

OCA at 08:04, 2 May 2018

OCA — Wed, 02 May 2018 08:04:48 GMT

←Older revision		Revision as of 08:04, 2 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The entry ~~5y31~~ is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of human LGI1 and human ADAM22 complex==
		+	<StructureSection load='5y31' size='340' side='right' caption='[[5y31]], [[Resolution\|resolution]] 7.12Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5y31]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y31 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y31 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y31 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y31 OCA], [http://pdbe.org/5y31 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y31 RCSB], [http://www.ebi.ac.uk/pdbsum/5y31 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y31 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/LGI1_HUMAN LGI1_HUMAN]] Autosomal dominant epilepsy with auditory features. The disease is caused by mutations affecting the gene represented in this entry.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ADA22_HUMAN ADA22_HUMAN]] Probable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.<ref>PMID:12589811</ref> <ref>PMID:15882968</ref> <ref>PMID:16385342</ref> [[http://www.uniprot.org/uniprot/LGI1_HUMAN LGI1_HUMAN]] Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors (By similarity). Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival.<ref>PMID:15047712</ref> <ref>PMID:16518856</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Epilepsy is a common brain disorder throughout history. Epilepsy-related ligand-receptor complex, LGI1-ADAM22, regulates synaptic transmission and has emerged as a determinant of brain excitability, as their mutations and acquired LGI1 autoantibodies cause epileptic disorders in human. Here, we report the crystal structure of human LGI1-ADAM22 complex, revealing a 2:2 heterotetrameric assembly. The hydrophobic pocket of the C-terminal epitempin-repeat (EPTP) domain of LGI1 binds to the metalloprotease-like domain of ADAM22. The N-terminal leucine-rich repeat and EPTP domains of LGI1 mediate the intermolecular LGI1-LGI1 interaction. A pathogenic R474Q mutation of LGI1, which does not exceptionally affect either the secretion or the ADAM22 binding, is located in the LGI1-LGI1 interface and disrupts the higher-order assembly of the LGI1-ADAM22 complex in vitro and in a mouse model for familial epilepsy. These studies support the notion that the LGI1-ADAM22 complex functions as the trans-synaptic machinery for precise synaptic transmission.

-	~~Authors~~:	+	Structural basis of epilepsy-related ligand-receptor complex LGI1-ADAM22.,Yamagata A, Miyazaki Y, Yokoi N, Shigematsu H, Sato Y, Goto-Ito S, Maeda A, Goto T, Sanbo M, Hirabayashi M, Shirouzu M, Fukata Y, Fukata M, Fukai S Nat Commun. 2018 Apr 18;9(1):1546. doi: 10.1038/s41467-018-03947-w. PMID:29670100<ref>PMID:29670100</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5y31" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Fukai, S]]
		+	[[Category: Yamagata, A]]
		+	[[Category: Adam]]
		+	[[Category: Cell adhesion]]
		+	[[Category: Epilepsy]]
		+	[[Category: Eptp]]
		+	[[Category: Synapse]]
		+	[[Category: Wd40]]

OCA: Protected "5y31" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:04:48 GMT

Protected "5y31" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:04, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5y31 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 16:04:47 GMT

New page: '''Unreleased structure''' The entry 5y31 is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5y31 is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]