5y5a - Revision history

OCA at 10:21, 27 March 2024

OCA — Wed, 27 Mar 2024 10:21:44 GMT

←Older revision		Revision as of 10:21, 27 March 2024
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	==Crystal structure of Est1 and Cdc13==		==Crystal structure of Est1 and Cdc13==
-	<StructureSection load='5y5a' size='340' side='right' caption='[[5y5a]], [[Resolution\|resolution]] 2.21Å' scene=''>	+	<StructureSection load='5y5a' size='340' side='right'caption='[[5y5a]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y5a]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Candida_sphaerica Candida sphaerica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5A OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Y5A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y5a]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis_NRRL_Y-1140 Kluyveromyces lactis NRRL Y-1140]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y5A FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5y58\|5y58]], [[5y59\|5y59]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.206Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">KLLA0_F20702g ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=284590 Candida sphaerica]), KLLA0_F20922g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5a OCA], [https://pdbe.org/5y5a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y5a RCSB], [https://www.ebi.ac.uk/pdbsum/5y5a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5a ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5y5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5a OCA], [~~http~~://pdbe.org/5y5a PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5y5a RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5y5a PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5a ProSAT]</span></td></tr>	+
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/Q6CJ80_KLULA Q6CJ80_KLULA]
-	Telomerase maintains chromosome ends from humans to yeasts. Recruitment of yeast telomerase to telomeres occurs through its Ku and Est1 subunits via independent interactions with telomerase RNA (TLC1) and telomeric proteins Sir4 and Cdc13, respectively. However, the structures of the molecules comprising these telomerase-recruiting pathways remain unknown. Here, we report crystal structures of the Ku heterodimer and Est1 complexed with their key binding partners. Two major findings are as follows: (1) Ku specifically binds to telomerase RNA in a distinct, yet related, manner to how it binds DNA; and (2) Est1 employs two separate pockets to bind distinct motifs of Cdc13. The N-terminal Cdc13-binding site of Est1 cooperates with the TLC1-Ku-Sir4 pathway for telomerase recruitment, whereas the C-terminal interface is dispensable for binding Est1 in vitro yet is nevertheless essential for telomere maintenance in vivo. Overall, our results integrate previous models and provide fundamentally valuable structural information regarding telomere biology.	+
-		+
-	Structural Insights into Yeast Telomerase Recruitment to Telomeres.,Chen H, Xue J, Churikov D, Hass EP, Shi S, Lemon LD, Luciano P, Bertuch AA, Zappulla DC, Geli V, Wu J, Lei M Cell. 2018 Jan 11;172(1-2):331-343.e13. doi: 10.1016/~~j.cell.2017.12.008. Epub, 2017 Dec 28. PMID:29290466<ref>PMID:29290466<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 5y5a" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Candida sphaerica~~]]	+	[[Category: Kluyveromyces lactis NRRL Y-1140]]
-	[[Category: Chen, H]]	+	[[Category: Large Structures]]
-	[[Category: Lei, M]]	+	[[Category: Chen H]]
-	[[Category: Wu, J]]	+	[[Category: Lei M]]
-	[[Category: Xue, J]]	+	[[Category: Wu J]]
-	~~[[Category: Protein binding]]~~	+	[[Category: Xue J]]
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Telomerase]]~~	+
-	~~[[Category: Telomere~~]]	+

OCA at 06:46, 7 February 2018

OCA — Wed, 07 Feb 2018 06:46:48 GMT

←Older revision		Revision as of 06:46, 7 February 2018
Line 3:		Line 3:
	<StructureSection load='5y5a' size='340' side='right' caption='[[5y5a]], [[Resolution\|resolution]] 2.21Å' scene=''>		<StructureSection load='5y5a' size='340' side='right' caption='[[5y5a]], [[Resolution\|resolution]] 2.21Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5y5a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y5A FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5y5a]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_sphaerica Candida sphaerica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y5A FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5y58\|5y58]], [[5y59\|5y59]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5y58\|5y58]], [[5y59\|5y59]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KLLA0_F20702g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica]), KLLA0_F20922g ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284590 Candida sphaerica])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5a OCA], [http://pdbe.org/5y5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y5a RCSB], [http://www.ebi.ac.uk/pdbsum/5y5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5a ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5a OCA], [http://pdbe.org/5y5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y5a RCSB], [http://www.ebi.ac.uk/pdbsum/5y5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5a ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Telomerase maintains chromosome ends from humans to yeasts. Recruitment of yeast telomerase to telomeres occurs through its Ku and Est1 subunits via independent interactions with telomerase RNA (TLC1) and telomeric proteins Sir4 and Cdc13, respectively. However, the structures of the molecules comprising these telomerase-recruiting pathways remain unknown. Here, we report crystal structures of the Ku heterodimer and Est1 complexed with their key binding partners. Two major findings are as follows: (1) Ku specifically binds to telomerase RNA in a distinct, yet related, manner to how it binds DNA; and (2) Est1 employs two separate pockets to bind distinct motifs of Cdc13. The N-terminal Cdc13-binding site of Est1 cooperates with the TLC1-Ku-Sir4 pathway for telomerase recruitment, whereas the C-terminal interface is dispensable for binding Est1 in vitro yet is nevertheless essential for telomere maintenance in vivo. Overall, our results integrate previous models and provide fundamentally valuable structural information regarding telomere biology.
		+
		+	Structural Insights into Yeast Telomerase Recruitment to Telomeres.,Chen H, Xue J, Churikov D, Hass EP, Shi S, Lemon LD, Luciano P, Bertuch AA, Zappulla DC, Geli V, Wu J, Lei M Cell. 2018 Jan 11;172(1-2):331-343.e13. doi: 10.1016/j.cell.2017.12.008. Epub, 2017 Dec 28. PMID:29290466<ref>PMID:29290466</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5y5a" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candida sphaerica]]
	[[Category: Chen, H]]		[[Category: Chen, H]]
	[[Category: Lei, M]]		[[Category: Lei, M]]

OCA at 06:22, 20 December 2017

OCA — Wed, 20 Dec 2017 06:22:11 GMT

←Older revision		Revision as of 06:22, 20 December 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5y5a is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Est1 and Cdc13==
-		+	<StructureSection load='5y5a' size='340' side='right' caption='[[5y5a]], [[Resolution\|resolution]] 2.21Å' scene=''>
-	~~Authors~~: ~~Chen~~, H., ~~Xue~~, J., ~~Wu, J~~., ~~Lei, M~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5y5a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y5A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y5A FirstGlance]. <br>
-	~~Description: Crystal structure of Est1 and Cdc13~~	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5y58\|5y58]], [[5y59\|5y59]]</td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y5a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y5a OCA], [http://pdbe.org/5y5a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y5a RCSB], [http://www.ebi.ac.uk/pdbsum/5y5a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y5a ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, H]]
	[[Category: Lei, M]]		[[Category: Lei, M]]
		+	[[Category: Wu, J]]
	[[Category: Xue, J]]		[[Category: Xue, J]]
-	[[Category: ~~Chen, H~~]]	+	[[Category: Protein binding]]
-	[[Category: ~~Wu, J~~]]	+	[[Category: Protein-protein complex]]
		+	[[Category: Telomerase]]
		+	[[Category: Telomere]]

OCA: Protected "5y5a" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:09:14 GMT

Protected "5y5a" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:09, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5y5a is ON HOLD until Paper Publication Authors: Chen, H., Xue, J., Wu, J., Lei, M. Description: Crystal structure of Est1 and Cdc13 [[Category: Un...

OCA — Wed, 27 Sep 2017 16:09:13 GMT

New page: '''Unreleased structure''' The entry 5y5a is ON HOLD until Paper Publication Authors: Chen, H., Xue, J., Wu, J., Lei, M. Description: Crystal structure of Est1 and Cdc13 [[Category: Un...

New page

'''Unreleased structure'''

The entry 5y5a is ON HOLD until Paper Publication

Authors: Chen, H., Xue, J., Wu, J., Lei, M.

Description: Crystal structure of Est1 and Cdc13
[[Category: Unreleased Structures]]
[[Category: Lei, M]]
[[Category: Xue, J]]
[[Category: Chen, H]]
[[Category: Wu, J]]