5ybx - Revision history

OCA at 08:31, 26 December 2018

OCA — Wed, 26 Dec 2018 08:31:32 GMT

←Older revision		Revision as of 08:31, 26 December 2018
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	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
-	~~The dynamic association~~ of chromosomes with the nuclear envelope (NE) is ~~essential for chromosome maintenance~~. ~~Schizosaccharomyces pombe~~ inner nuclear membrane protein Bqt4 plays ~~a critical role~~ in connecting telomeres to the NE, ~~mainly through a direct interaction with~~ the ~~telomeric protein Rap1.~~ Bqt4 ~~also interacts with Lem2 for pericentric heterochromatin maintenance. How Bqt4 coordinates the interactions with different proteins to exert their functions~~ is ~~unclear~~. Here, we report the crystal ~~structures~~ of the N-terminal domain of Bqt4 ~~in complexes with Bqt4-binding motifs from Rap1, Lem2, and Sad1~~. The ~~structural, biochemical and cellular analyses reveal that the~~ N-terminal domain of Bqt4 is a ~~protein~~-interaction ~~module that recognizes a consensus motif and plays essential roles~~ in telomere-NE ~~association and meiosis progression~~. ~~Phosphorylation~~ of Bqt4~~-interacting proteins~~ may ~~act as a switch~~ to ~~regulate these interactions during cell cycles. Our studies provide structural insights into~~ the ~~identification~~ and ~~regulation of Bqt4~~-~~mediated interactions~~.	+	Telomeres, the protective caps at the end of the chromosomes, are often associated with the nuclear envelope (NE). Telomere positioning to the NE is dynamically regulated during mitosis and meiosis. One inner nuclear membrane protein, Bqt4, in Schizosaccharomyces pombe plays essential roles in connecting telomeres to the NE. However, the structural basis of Bqt4 in mediating telomere-NE association is not clear. Here, we report the crystal structure of the N-terminal domain of Bqt4. The N-terminal domain of Bqt4 structurally resembles the APSES-family DNA-binding domain and has a moderate double-stranded DNA-binding activity. Disruption of Bqt4-DNA interaction results in telomere detachment from the NE. These data suggest that the DNA-binding activity of Bqt4 may function to prime the chromosome onto the NE and promote telomere-NE association.

-	~~Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein~~ Bqt4 in ~~fission yeast~~.,Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y ~~Nucleic Acids Res~~. 2018 ~~Nov 20~~. pii: ~~5193554~~. doi: 10.~~1093~~/~~nar/gky1186~~. PMID:~~30462301~~<ref>PMID:~~30462301~~</ref>	+	The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope.,Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y Structure. 2018 Oct 30. pii: S0969-2126(18)30371-X. doi:, 10.1016/j.str.2018.10.010. PMID:30503780<ref>PMID:30503780</ref>

	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>

OCA at 07:05, 12 December 2018

OCA — Wed, 12 Dec 2018 07:05:30 GMT

←Older revision		Revision as of 07:05, 12 December 2018
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	<StructureSection load='5ybx' size='340' side='right' caption='[[5ybx]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5ybx' size='340' side='right' caption='[[5ybx]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ybx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YBX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ybx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Fission_yeast Fission yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YBX FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bqt4, SPBC19C7.10 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=284812 Fission yeast])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ybx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ybx OCA], [http://pdbe.org/5ybx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ybx RCSB], [http://www.ebi.ac.uk/pdbsum/5ybx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ybx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ybx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ybx OCA], [http://pdbe.org/5ybx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ybx RCSB], [http://www.ebi.ac.uk/pdbsum/5ybx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ybx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO]] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref>		[[http://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO]] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dynamic association of chromosomes with the nuclear envelope (NE) is essential for chromosome maintenance. Schizosaccharomyces pombe inner nuclear membrane protein Bqt4 plays a critical role in connecting telomeres to the NE, mainly through a direct interaction with the telomeric protein Rap1. Bqt4 also interacts with Lem2 for pericentric heterochromatin maintenance. How Bqt4 coordinates the interactions with different proteins to exert their functions is unclear. Here, we report the crystal structures of the N-terminal domain of Bqt4 in complexes with Bqt4-binding motifs from Rap1, Lem2, and Sad1. The structural, biochemical and cellular analyses reveal that the N-terminal domain of Bqt4 is a protein-interaction module that recognizes a consensus motif and plays essential roles in telomere-NE association and meiosis progression. Phosphorylation of Bqt4-interacting proteins may act as a switch to regulate these interactions during cell cycles. Our studies provide structural insights into the identification and regulation of Bqt4-mediated interactions.
		+
		+	Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast.,Hu C, Inoue H, Sun W, Takeshita Y, Huang Y, Xu Y, Kanoh J, Chen Y Nucleic Acids Res. 2018 Nov 20. pii: 5193554. doi: 10.1093/nar/gky1186. PMID:30462301<ref>PMID:30462301</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ybx" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Fission yeast]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Hu, C]]		[[Category: Hu, C]]

OCA at 19:37, 19 September 2018

OCA — Wed, 19 Sep 2018 19:37:40 GMT

←Older revision		Revision as of 19:37, 19 September 2018
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-	'''Unreleased structure'''

-	~~The entry 5ybx is ON HOLD until Paper Publication~~	+	==Crystal structure of the N-terminal domain of Bqt4 in S.pombe==
-		+	<StructureSection load='5ybx' size='340' side='right' caption='[[5ybx]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~Authors: Hu, C., Chen, Y.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ybx]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YBX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YBX FirstGlance]. <br>
-	~~Description:~~ Crystal structure of the N-terminal domain of Bqt4 in S.pombe	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ybx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ybx OCA], [http://pdbe.org/5ybx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ybx RCSB], [http://www.ebi.ac.uk/pdbsum/5ybx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ybx ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BQT4_SCHPO BQT4_SCHPO]] Connects telomeres to the nuclear envelop (NE) during both vegetative growth and meiosis. This connection ensures clustering of telomeres to the spindle pole body (SPB) when cells enter meiotic prophase.<ref>PMID:19948484</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Hu, C]]		[[Category: Hu, C]]
		+	[[Category: Chromosome organization]]
		+	[[Category: Dna binding protein]]
		+	[[Category: Nuclear envelope]]
		+	[[Category: Telomere bouquet]]

OCA: Protected "5ybx" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:23:17 GMT

Protected "5ybx" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:23, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ybx is ON HOLD until Paper Publication Authors: Hu, C., Chen, Y. Description: Crystal structure of the N-terminal domain of Bqt4 in S.pombe [[Cate...

OCA — Wed, 27 Sep 2017 16:23:16 GMT

New page: '''Unreleased structure''' The entry 5ybx is ON HOLD until Paper Publication Authors: Hu, C., Chen, Y. Description: Crystal structure of the N-terminal domain of Bqt4 in S.pombe [[Cate...

New page

'''Unreleased structure'''

The entry 5ybx is ON HOLD until Paper Publication

Authors: Hu, C., Chen, Y.

Description: Crystal structure of the N-terminal domain of Bqt4 in S.pombe
[[Category: Unreleased Structures]]
[[Category: Chen, Y]]
[[Category: Hu, C]]