5ycw - Revision history

OCA at 08:26, 22 November 2023

OCA — Wed, 22 Nov 2023 08:26:56 GMT

←Older revision		Revision as of 08:26, 22 November 2023
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	==Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin==		==Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin==
-	<StructureSection load='5ycw' size='340' side='right' caption='[[5ycw]], [[Resolution\|resolution]] 2.29Å' scene=''>	+	<StructureSection load='5ycw' size='340' side='right'caption='[[5ycw]], [[Resolution\|resolution]] 2.29Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ycw]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Diocu Diocu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YCW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YCW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ycw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Dioscoreophyllum_cumminsii Dioscoreophyllum cumminsii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YCW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YCW FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ycw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ycw OCA], [~~http~~://pdbe.org/5ycw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ycw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ycw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ycw ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.285Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ycw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ycw OCA], [https://pdbe.org/5ycw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ycw RCSB], [https://www.ebi.ac.uk/pdbsum/5ycw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ycw ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MONA_DIOCU MONA_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.[https://www.uniprot.org/uniprot/MONB_DIOCU MONB_DIOCU] Taste-modifying protein; intensely sweet-tasting protein.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Diocu~~]]	+	[[Category: Dioscoreophyllum cumminsii]]
-	[[Category: Das, R]]	+	[[Category: Large Structures]]
-	[[Category: Gosavi, S]]	+	[[Category: Das R]]
-	[[Category: Nandwani, N]]	+	[[Category: Gosavi S]]
-	[[Category: Surana, P]]	+	[[Category: Nandwani N]]
-	[[Category: Udgaonkar~~, J B]]~~	+	[[Category: Surana P]]
-	~~[[Category: Domain swapped dimer]]~~	+	[[Category: Udgaonkar JB]]
-	~~[[Category: Loop mutation]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Qvvag motif]]~~	+
-	~~[[Category: Single-chain monellin~~]]	+

OCA at 09:40, 13 February 2019

OCA — Wed, 13 Feb 2019 09:40:58 GMT

←Older revision		Revision as of 09:40, 13 February 2019
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	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ycw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ycw OCA], [http://pdbe.org/5ycw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ycw RCSB], [http://www.ebi.ac.uk/pdbsum/5ycw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ycw ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ycw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ycw OCA], [http://pdbe.org/5ycw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ycw RCSB], [http://www.ebi.ac.uk/pdbsum/5ycw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ycw ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Domain swapping is the process by which identical monomeric proteins exchange structural elements to generate dimers/oligomers. Although engineered domain swapping is a compelling strategy for protein assembly, its application has been limited due to the lack of simple and reliable design approaches. Here, we demonstrate that the hydrophobic five-residue 'cystatin motif' (QVVAG) from the domain-swapping protein Stefin B, when engineered into a solvent-exposed, tight surface loop between two beta-strands prevents the loop from folding back upon itself, and drives domain swapping in non-domain-swapping proteins. High-resolution structural studies demonstrate that engineering the QVVAG stretch independently into various surface loops of four structurally distinct non-domain-swapping proteins enabled the design of different modes of domain swapping in these proteins, including single, double and open-ended domain swapping. These results suggest that the introduction of the QVVAG motif can be used as a mutational approach for engineering domain swapping in diverse beta-hairpin proteins.
		+
		+	A five-residue motif for the design of domain swapping in proteins.,Nandwani N, Surana P, Negi H, Mascarenhas NM, Udgaonkar JB, Das R, Gosavi S Nat Commun. 2019 Jan 28;10(1):452. doi: 10.1038/s41467-019-08295-x. PMID:30692525<ref>PMID:30692525</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5ycw" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 20:21, 2 December 2018

OCA — Sun, 02 Dec 2018 20:21:52 GMT

←Older revision		Revision as of 20:21, 2 December 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5ycw ~~is ON HOLD until Nov 20 2019~~	+	==Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin==
-		+	<StructureSection load='5ycw' size='340' side='right' caption='[[5ycw]], [[Resolution\|resolution]] 2.29Å' scene=''>
-	~~Authors~~: ~~Surana~~, P., ~~Nandwani, N~~., ~~Udgaonkar, J~~.B., ~~Gosavi, S~~., ~~Das, R~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ycw]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Diocu Diocu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YCW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YCW FirstGlance]. <br>
-	~~Description: Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ycw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ycw OCA], [http://pdbe.org/5ycw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ycw RCSB], [http://www.ebi.ac.uk/pdbsum/5ycw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ycw ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Udgaonkar~~, ~~J.B~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Diocu]]
		+	[[Category: Das, R]]
	[[Category: Gosavi, S]]		[[Category: Gosavi, S]]
	[[Category: Nandwani, N]]		[[Category: Nandwani, N]]
	[[Category: Surana, P]]		[[Category: Surana, P]]
-	[[Category: ~~Das~~, R]]	+	[[Category: Udgaonkar, J B]]
		+	[[Category: Domain swapped dimer]]
		+	[[Category: Loop mutation]]
		+	[[Category: Plant protein]]
		+	[[Category: Qvvag motif]]
		+	[[Category: Single-chain monellin]]

OCA at 06:15, 14 November 2018

OCA — Wed, 14 Nov 2018 06:15:16 GMT

←Older revision		Revision as of 06:15, 14 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ycw is ON HOLD until Nov 13 2019	+	The entry 5ycw is ON HOLD until Nov 20 2019

	Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R.		Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R.
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	Description: Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin		Description: Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
		+	[[Category: Udgaonkar, J.B]]
	[[Category: Gosavi, S]]		[[Category: Gosavi, S]]
	[[Category: Nandwani, N]]		[[Category: Nandwani, N]]
	[[Category: Surana, P]]		[[Category: Surana, P]]
	[[Category: Das, R]]		[[Category: Das, R]]
-	[[Category: Udgaonkar, J.B]]

OCA at 17:59, 19 September 2018

OCA — Wed, 19 Sep 2018 17:59:07 GMT

←Older revision		Revision as of 17:59, 19 September 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ycw is ON HOLD until ~~Paper Publication~~	+	The entry 5ycw is ON HOLD until Nov 13 2019

	Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R.		Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Gosavi, S]]		[[Category: Gosavi, S]]
-	[[Category: Das, R]]
-	[[Category: Udgaonkar, J.B]]
	[[Category: Nandwani, N]]		[[Category: Nandwani, N]]
	[[Category: Surana, P]]		[[Category: Surana, P]]
		+	[[Category: Das, R]]
		+	[[Category: Udgaonkar, J.B]]

OCA: Protected "5ycw" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:25:20 GMT

Protected "5ycw" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:25, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 5ycw is ON HOLD until Paper Publication Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R. Description: Double domain swapped ...

OCA — Wed, 27 Sep 2017 16:25:19 GMT

New page: '''Unreleased structure''' The entry 5ycw is ON HOLD until Paper Publication Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R. Description: Double domain swapped ...

New page

'''Unreleased structure'''

The entry 5ycw is ON HOLD until Paper Publication

Authors: Surana, P., Nandwani, N., Udgaonkar, J.B., Gosavi, S., Das, R.

Description: Double domain swapped dimer of engineered hairpin loop1 and loop3 mutant in Single-chain Monellin
[[Category: Unreleased Structures]]
[[Category: Gosavi, S]]
[[Category: Das, R]]
[[Category: Udgaonkar, J.B]]
[[Category: Nandwani, N]]
[[Category: Surana, P]]