5yi9 - Revision history

OCA at 10:32, 14 June 2023

OCA — Wed, 14 Jun 2023 10:32:54 GMT

←Older revision		Revision as of 10:32, 14 June 2023
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	==Solution structure of the LEDGF/p75 IBD - JPO2 (aa 56-91) complex==		==Solution structure of the LEDGF/p75 IBD - JPO2 (aa 56-91) complex==
-	<StructureSection load='5yi9' size='340' side='right'caption='[[5yi9~~]], [[NMR_Ensembles_of_Models \| 40 NMR models~~]]' scene=''>	+	<StructureSection load='5yi9' size='340' side='right'caption='[[5yi9]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yi9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YI9 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YI9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yi9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YI9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yi9 OCA], [~~http~~://pdbe.org/5yi9 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yi9 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yi9 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yi9 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yi9 OCA], [https://pdbe.org/5yi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yi9 RCSB], [https://www.ebi.ac.uk/pdbsum/5yi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yi9 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN]] Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.	+	[https://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN] Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN]] Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.<ref>PMID:15642333</ref>	+	[https://www.uniprot.org/uniprot/CDA7L_HUMAN CDA7L_HUMAN] Plays a role in transcriptional regulation as a repressor that inhibits monoamine oxidase A (MAOA) activity and gene expression by binding to the promoter. Plays an important oncogenic role in mediating the full transforming effect of MYC in medulloblastoma cells. Involved in apoptotic signaling pathways; May act downstream of P38-kinase and BCL-2, but upstream of CASP3/caspase-3 as well as CCND1/cyclin D1 and E2F1.<ref>PMID:15654081</ref> <ref>PMID:15994933</ref> <ref>PMID:16829576</ref> [https://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN] Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.<ref>PMID:15642333</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Veverka, V]]	+	[[Category: Veverka V]]
-	~~[[Category: Epigenetic]]~~	+
-	~~[[Category: Leukemia]]~~	+
-	~~[[Category: Protein-protein complex]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 09:20, 21 October 2020

OCA — Wed, 21 Oct 2020 09:20:11 GMT

←Older revision		Revision as of 09:20, 21 October 2020
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	==Solution structure of the LEDGF/p75 IBD - JPO2 (aa 56-91) complex==		==Solution structure of the LEDGF/p75 IBD - JPO2 (aa 56-91) complex==
-	<StructureSection load='5yi9' size='340' side='right' caption='[[5yi9]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>	+	<StructureSection load='5yi9' size='340' side='right'caption='[[5yi9]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yi9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YI9 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YI9 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yi9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YI9 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YI9 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yi9 OCA], [http://pdbe.org/5yi9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yi9 RCSB], [http://www.ebi.ac.uk/pdbsum/5yi9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yi9 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yi9 OCA], [http://pdbe.org/5yi9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yi9 RCSB], [http://www.ebi.ac.uk/pdbsum/5yi9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yi9 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Veverka, V]]		[[Category: Veverka, V]]
	[[Category: Epigenetic]]		[[Category: Epigenetic]]

OCA at 16:08, 15 August 2018

OCA — Wed, 15 Aug 2018 16:08:22 GMT

←Older revision		Revision as of 16:08, 15 August 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5yi9 is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Solution structure of the LEDGF/p75 IBD - JPO2 (aa 56-91) complex==
		+	<StructureSection load='5yi9' size='340' side='right' caption='[[5yi9]], [[NMR_Ensembles_of_Models \| 40 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5yi9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YI9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YI9 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yi9 OCA], [http://pdbe.org/5yi9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yi9 RCSB], [http://www.ebi.ac.uk/pdbsum/5yi9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yi9 ProSAT]</span></td></tr>
		+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN]] Note=A chromosomal aberration involving PSIP1 is associated with pediatric acute myeloid leukemia (AML) with intermediate characteristics between M2-M3 French-American-British (FAB) subtypes. Translocation t(9;11)(p22;p15) with NUP98. The chimeric transcript is an in-frame fusion of NUP98 exon 8 to PSIP1/LEDGF exon 4.
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PSIP1_HUMAN PSIP1_HUMAN]] Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.<ref>PMID:15642333</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lens epithelium-derived growth factor/p75 (LEDGF/p75, or PSIP1) is a transcriptional coactivator that tethers other proteins to gene bodies. The chromatin tethering function of LEDGF/p75 is hijacked by HIV integrase to ensure viral integration at sites of active transcription. LEDGF/p75 is also important for the development of mixed-lineage leukemia (MLL), where it tethers the MLL1 fusion complex at aberrant MLL targets, inducing malignant transformation. However, little is known about how the LEDGF/p75 protein interaction network is regulated. Here, we obtained solution structures of the complete interfaces between the LEDGF/p75 integrase binding domain (IBD) and its cellular binding partners and validated another binding partner, Mediator subunit 1 (MED1). We reveal that structurally conserved IBD-binding motifs (IBMs) on known LEDGF/p75 binding partners can be regulated by phosphorylation, permitting switching between low- and high-affinity states. Finally, we show that elimination of IBM phosphorylation sites on MLL1 disrupts the oncogenic potential of primary MLL1-rearranged leukemic cells. Our results demonstrate that kinase-dependent phosphorylation of MLL1 represents a previously unknown oncogenic dependency that may be harnessed in the treatment of MLL-rearranged leukemia.

-	~~Authors~~:	+	Affinity switching of the LEDGF/p75 IBD interactome is governed by kinase-dependent phosphorylation.,Sharma S, Cermakova K, De Rijck J, Demeulemeester J, Fabry M, El Ashkar S, Van Belle S, Lepsik M, Tesina P, Duchoslav V, Novak P, Hubalek M, Srb P, Christ F, Rezacova P, Hodges HC, Debyser Z, Veverka V Proc Natl Acad Sci U S A. 2018 Jul 11. pii: 1803909115. doi:, 10.1073/pnas.1803909115. PMID:29997176<ref>PMID:29997176</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5yi9" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Veverka, V]]
		+	[[Category: Epigenetic]]
		+	[[Category: Leukemia]]
		+	[[Category: Protein-protein complex]]
		+	[[Category: Transcription]]

OCA at 04:43, 1 November 2017

OCA — Wed, 01 Nov 2017 04:43:17 GMT

←Older revision		Revision as of 04:43, 1 November 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5yi9 is ON HOLD	+	The entry 5yi9 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5yi9" [edit=sysop:move=sysop]

OCA — Wed, 11 Oct 2017 05:24:23 GMT

Protected "5yi9" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:24, 11 October 2017

OCA: New page: '''Unreleased structure''' The entry 5yi9 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Oct 2017 05:24:22 GMT

New page: '''Unreleased structure''' The entry 5yi9 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5yi9 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]