5yl7 - Revision history

OCA at 09:22, 21 October 2020

OCA — Wed, 21 Oct 2020 09:22:02 GMT

←Older revision		Revision as of 09:22, 21 October 2020
Line 1:		Line 1:

	==Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)==		==Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)==
-	<StructureSection load='5yl7' size='340' side='right' caption='[[5yl7]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='5yl7' size='340' side='right'caption='[[5yl7]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_18437 Dsm 18437] and [http://en.wikipedia.org/wiki/Pseudoalteromonas_arctica Pseudoalteromonas arctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YL7 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_18437 Dsm 18437] and [http://en.wikipedia.org/wiki/Pseudoalteromonas_arctica Pseudoalteromonas arctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YL7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YL7 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yl7 OCA], [http://pdbe.org/5yl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yl7 RCSB], [http://www.ebi.ac.uk/pdbsum/5yl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yl7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yl7 OCA], [http://pdbe.org/5yl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yl7 RCSB], [http://www.ebi.ac.uk/pdbsum/5yl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yl7 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Dsm 18437]]		[[Category: Dsm 18437]]
		+	[[Category: Large Structures]]
	[[Category: Pseudoalteromonas arctica]]		[[Category: Pseudoalteromonas arctica]]
	[[Category: Lee, C W]]		[[Category: Lee, C W]]

OCA at 07:47, 12 September 2018

OCA — Wed, 12 Sep 2018 07:47:38 GMT

←Older revision		Revision as of 07:47, 12 September 2018
Line 3:		Line 3:
	<StructureSection load='5yl7' size='340' side='right' caption='[[5yl7]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='5yl7' size='340' side='right' caption='[[5yl7]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Pseudoalteromonas_arctica Pseudoalteromonas arctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_18437 Dsm 18437] and [http://en.wikipedia.org/wiki/Pseudoalteromonas_arctica Pseudoalteromonas arctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YL7 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yl7 OCA], [http://pdbe.org/5yl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yl7 RCSB], [http://www.ebi.ac.uk/pdbsum/5yl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yl7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yl7 OCA], [http://pdbe.org/5yl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yl7 RCSB], [http://www.ebi.ac.uk/pdbsum/5yl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yl7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Enzymes isolated from organisms found in cold habitats generally exhibit higher catalytic activity at low temperatures than their mesophilic homologs and are therefore known as cold-active enzymes. Cold-active proteases are very useful in a variety of biotechnological applications, particularly as active ingredients in laundry and dishwashing detergents, where they provide strong protein-degrading activity in cold water. We identified a cold-active protease (Pro21717) from a psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, and determined the crystal structure of its catalytic domain (CD) at a resolution of 1.4 A. The Pro21717-CD structure shows a conserved subtilisin-like fold with a typical catalytic triad (Asp185, His244, and Ser425) and contains four calcium ions and three disulfide bonds. Interestingly, we observed an unexpected electron density at the substrate-binding site from a co-purified peptide. Although the sequence of this peptide is unknown, analysis of the peptide-complexed structure nonetheless provides some indication of the substrate recognition and binding mode of Pro21717. Moreover, various parameters, including a wide substrate pocket size, an abundant active-site loop content, and a flexible structure provide potential explanations for the cold-adapted properties of Pro21717. In conclusion, this is first structural characterization of a cold-adapted subtilisin-like protease, and these findings provide a structural and functional basis for industrial applications of Pro21717 as a cold-active laundry or dishwashing detergent enzyme.
		+
		+	Crystal structure of a cold-active protease (Pro21717) from the psychrophilic bacterium, Pseudoalteromonas arctica PAMC 21717, at 1.4 A resolution: Structural adaptations to cold and functional analysis of a laundry detergent enzyme.,Park HJ, Lee CW, Kim D, Do H, Han SJ, Kim JE, Koo BH, Lee JH, Yim JH PLoS One. 2018 Feb 21;13(2):e0191740. doi: 10.1371/journal.pone.0191740., eCollection 2018. PMID:29466378<ref>PMID:29466378</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5yl7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dsm 18437]]
	[[Category: Pseudoalteromonas arctica]]		[[Category: Pseudoalteromonas arctica]]
	[[Category: Lee, C W]]		[[Category: Lee, C W]]

OCA at 05:54, 31 January 2018

OCA — Wed, 31 Jan 2018 05:54:43 GMT

←Older revision		Revision as of 05:54, 31 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5yl7 is ~~ON HOLD~~	+	==Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)==
-		+	<StructureSection load='5yl7' size='340' side='right' caption='[[5yl7]], [[Resolution\|resolution]] 1.40Å' scene=''>
-	~~Authors~~: ~~Lee~~, J.H., ~~Lee~~, C.W.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5yl7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Pseudoalteromonas_arctica Pseudoalteromonas arctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YL7 FirstGlance]. <br>
-	~~Description~~: ~~Proteases from~~ Pseudoalteromonas arctica ~~PAMC 21717 (Pro21717)~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
-	[[Category: Lee, J.H]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yl7 OCA], [http://pdbe.org/5yl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yl7 RCSB], [http://www.ebi.ac.uk/pdbsum/5yl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yl7 ProSAT]</span></td></tr>
-	[[Category: ~~Lee, C.W~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Pseudoalteromonas arctica]]
		+	[[Category: Lee, C W]]
		+	[[Category: Lee, J H]]
		+	[[Category: Hydrolase]]
		+	[[Category: Psychrophilic bacterium]]
		+	[[Category: Serine protease]]

OCA at 17:09, 24 January 2018

OCA — Wed, 24 Jan 2018 17:09:17 GMT

←Older revision		Revision as of 17:09, 24 January 2018
Line 7:		Line 7:
	Description: Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)		Description: Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Lee, C.W]]
	[[Category: Lee, J.H]]		[[Category: Lee, J.H]]
		+	[[Category: Lee, C.W]]

OCA: Protected "5yl7" [edit=sysop:move=sysop]

OCA — Wed, 25 Oct 2017 05:40:23 GMT

Protected "5yl7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:40, 25 October 2017

OCA: New page: '''Unreleased structure''' The entry 5yl7 is ON HOLD Authors: Lee, J.H., Lee, C.W. Description: Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717) [[Category: Unreleased St...

OCA — Wed, 25 Oct 2017 05:40:21 GMT

New page: '''Unreleased structure''' The entry 5yl7 is ON HOLD Authors: Lee, J.H., Lee, C.W. Description: Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717) [[Category: Unreleased St...

New page

'''Unreleased structure'''

The entry 5yl7 is ON HOLD

Authors: Lee, J.H., Lee, C.W.

Description: Proteases from Pseudoalteromonas arctica PAMC 21717 (Pro21717)
[[Category: Unreleased Structures]]
[[Category: Lee, C.W]]
[[Category: Lee, J.H]]