5ynj - Revision history

OCA at 08:33, 22 November 2023

OCA — Wed, 22 Nov 2023 08:33:36 GMT

←Older revision		Revision as of 08:33, 22 November 2023
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	<StructureSection load='5ynj' size='340' side='right'caption='[[5ynj]], [[Resolution\|resolution]] 2.04Å' scene=''>		<StructureSection load='5ynj' size='340' side='right'caption='[[5ynj]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ynj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YNJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YNJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ynj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_betacoronavirus_2c_EMC/2012 Human betacoronavirus 2c EMC/2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YNJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YNJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.043Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5ynj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ynj OCA], [~~http~~://pdbe.org/5ynj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ynj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ynj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ynj ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ynj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ynj OCA], [https://pdbe.org/5ynj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ynj RCSB], [https://www.ebi.ac.uk/pdbsum/5ynj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ynj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~K4LC41_9BETC K4LC41_9BETC~~]] ~~Nsp7~~-~~nsp8~~ hexadecamer may ~~possibly confer processivity to the polymerase~~, ~~maybe~~ by binding to dsRNA ~~or by producing primers utilized~~ by the ~~latter~~ (By similarity).[~~SAAS~~:~~SAAS014829_004_014545~~] ~~Nsp9~~ is a ~~ssRNA-binding~~ protein ~~(By similarity)~~.[~~SAAS~~:~~SAAS014829_004_001449~~]	+	[https://www.uniprot.org/uniprot/K0BWD0_MERS K0BWD0_MERS] Cleaves the C-terminus of replicase polyprotein at 11 sites. Recognizes substrates containing the core sequence [ILMVF]-Q-\|-[SGACN]. Also able to bind an ADP-ribose-1''-phosphate (ADRP).[ARBA:ARBA00002223] Enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity.[ARBA:ARBA00003995] Forms a hexadecamer with nsp7 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[ARBA:ARBA00002182] Forms a hexadecamer with nsp8 (8 subunits of each) that may participate in viral replication by acting as a primase. Alternatively, may synthesize substantially longer products than oligonucleotide primers.[ARBA:ARBA00003443] May participate in viral replication by acting as a ssRNA-binding protein.[ARBA:ARBA00002012] May play a role in the modulation of host cell survival signaling pathway by interacting with host PHB and PHB2. Indeed, these two proteins play a role in maintaining the functional integrity of the mitochondria and protecting cells from various stresses.[ARBA:ARBA00003115] Methyltransferase that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.[ARBA:ARBA00002840] Multi-functional protein with a zinc-binding domain in N-terminus displaying RNA and DNA duplex-unwinding activities with 5' to 3' polarity. Activity of helicase is dependent on magnesium.[ARBA:ARBA00002960] Participates in the assembly of virally-induced cytoplasmic double-membrane vesicles necessary for viral replication.[ARBA:ARBA00003070] Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation.[ARBA:ARBA00002697] Plays a role in the initial induction of autophagosomes from host reticulum endoplasmic. Later, limits the expansion of these phagosomes that are no longer able to deliver viral components to lysosomes.[ARBA:ARBA00003748] Plays a role in viral transcription/replication and prevents the simultaneous activation of host cell dsRNA sensors, such as MDA5/IFIH1, OAS, and PKR (By similarity). Acts by degrading the 5'-polyuridines generated during replication of the poly(A) region of viral genomic and subgenomic RNAs. Catalyzes a two-step reaction in which a 2'3'-cyclic phosphate (2'3'-cP) is first generated by 2'-O transesterification, which is then hydrolyzed to a 3'-phosphate (3'-P) (By similarity). If not degraded, poly(U) RNA would hybridize with poly(A) RNA tails and activate host dsRNA sensors.[ARBA:ARBA00025521] Responsible for replication and transcription of the viral RNA genome.[ARBA:ARBA00003927] The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.[ARBA:ARBA00003368]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human betacoronavirus 2c EMC/2012]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, Y]]	+	[[Category: Chen Y]]
-	[[Category: Fan~~, C P~~]]	+	[[Category: Fan CP]]
-	[[Category: Guo~~, D Y~~]]	+	[[Category: Guo DY]]
-	[[Category: Ke~~, Z H~~]]	+	[[Category: Ke ZH]]
-	[[Category: Wei~~, S M~~]]	+	[[Category: Wei SM]]
-	[[Category: Yang, L]]	+	[[Category: Yang L]]
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Inhibitor]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:23, 21 October 2020

OCA — Wed, 21 Oct 2020 09:23:17 GMT

←Older revision		Revision as of 09:23, 21 October 2020
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	==Crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG==		==Crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG==
-	<StructureSection load='5ynj' size='340' side='right' caption='[[5ynj]], [[Resolution\|resolution]] 2.04Å' scene=''>	+	<StructureSection load='5ynj' size='340' side='right'caption='[[5ynj]], [[Resolution\|resolution]] 2.04Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ynj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YNJ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YNJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ynj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YNJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YNJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ynj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ynj OCA], [http://pdbe.org/5ynj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ynj RCSB], [http://www.ebi.ac.uk/pdbsum/5ynj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ynj ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ynj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ynj OCA], [http://pdbe.org/5ynj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ynj RCSB], [http://www.ebi.ac.uk/pdbsum/5ynj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ynj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chen, Y]]		[[Category: Chen, Y]]
	[[Category: Fan, C P]]		[[Category: Fan, C P]]

OCA at 06:27, 5 December 2018

OCA — Wed, 05 Dec 2018 06:27:54 GMT

←Older revision		Revision as of 06:27, 5 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ynj ~~is ON HOLD until Paper Publication~~	+	==Crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG==
-		+	<StructureSection load='5ynj' size='340' side='right' caption='[[5ynj]], [[Resolution\|resolution]] 2.04Å' scene=''>
-	~~Authors~~: ~~Wei, S~~.M., ~~Yang, L~~., ~~Ke, Z~~.H., ~~Chen~~, Y., ~~Guo, D~~.Y., ~~Fan, C~~.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5ynj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YNJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YNJ FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of MERS-CoV nsp16~~/~~nsp10 complex bound~~ to ~~m7GpppG~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ynj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ynj OCA], [http://pdbe.org/5ynj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ynj RCSB], [http://www.ebi.ac.uk/pdbsum/5ynj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ynj ProSAT]</span></td></tr>
-	[[Category: Ke, Z.H]]	+	</table>
-	[[Category: ~~Fan~~, ~~C.P~~]]	+	== Function ==
		+	[[http://www.uniprot.org/uniprot/K4LC41_9BETC K4LC41_9BETC]] Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter (By similarity).[SAAS:SAAS014829_004_014545] Nsp9 is a ssRNA-binding protein (By similarity).[SAAS:SAAS014829_004_001449]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y]]
		+	[[Category: Fan, C P]]
		+	[[Category: Guo, D Y]]
		+	[[Category: Ke, Z H]]
		+	[[Category: Wei, S M]]
	[[Category: Yang, L]]		[[Category: Yang, L]]
-	[[Category: ~~Guo, D.Y~~]]	+	[[Category: Complex]]
-	[[Category: ~~Chen, Y~~]]	+	[[Category: Inhibitor]]
-	[[Category: ~~Wei, S.M~~]]	+	[[Category: Transferase]]

OCA at 06:24, 22 November 2017

OCA — Wed, 22 Nov 2017 06:24:24 GMT

←Older revision		Revision as of 06:24, 22 November 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ynj is ON HOLD	+	The entry 5ynj is ON HOLD until Paper Publication

	Authors: Wei, S.M., Yang, L., Ke, Z.H., Chen, Y., Guo, D.Y., Fan, C.P.		Authors: Wei, S.M., Yang, L., Ke, Z.H., Chen, Y., Guo, D.Y., Fan, C.P.

-	Description: ~~crystal~~ structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG	+	Description: Crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Ke, Z.H]]		[[Category: Ke, Z.H]]

OCA: Protected "5ynj" [edit=sysop:move=sysop]

OCA — Wed, 01 Nov 2017 04:47:41 GMT

Protected "5ynj" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 1 November 2017

OCA: New page: '''Unreleased structure''' The entry 5ynj is ON HOLD Authors: Wei, S.M., Yang, L., Ke, Z.H., Chen, Y., Guo, D.Y., Fan, C.P. Description: crystal structure of MERS-CoV nsp16/nsp10 compl...

OCA — Wed, 01 Nov 2017 04:47:39 GMT

New page: '''Unreleased structure''' The entry 5ynj is ON HOLD Authors: Wei, S.M., Yang, L., Ke, Z.H., Chen, Y., Guo, D.Y., Fan, C.P. Description: crystal structure of MERS-CoV nsp16/nsp10 compl...

New page

'''Unreleased structure'''

The entry 5ynj is ON HOLD

Authors: Wei, S.M., Yang, L., Ke, Z.H., Chen, Y., Guo, D.Y., Fan, C.P.

Description: crystal structure of MERS-CoV nsp16/nsp10 complex bound to m7GpppG
[[Category: Unreleased Structures]]
[[Category: Ke, Z.H]]
[[Category: Fan, C.P]]
[[Category: Yang, L]]
[[Category: Guo, D.Y]]
[[Category: Chen, Y]]
[[Category: Wei, S.M]]