5yq3 - Revision history

OCA at 10:33, 14 June 2023

OCA — Wed, 14 Jun 2023 10:33:56 GMT

←Older revision		Revision as of 10:33, 14 June 2023
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	==Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter==		==Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter==
-	<StructureSection load='5yq3' size='340' side='right'caption='[[5yq3~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5yq3' size='340' side='right'caption='[[5yq3]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yq3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ3 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YQ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yq3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YQ3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5yq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq3 OCA], [~~http~~://pdbe.org/5yq3 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yq3 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yq3 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq3 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq3 OCA], [https://pdbe.org/5yq3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yq3 RCSB], [https://www.ebi.ac.uk/pdbsum/5yq3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq3 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/PP16A_ARATH PP16A_ARATH] Binds to both sense and antisense RNA (PubMed:29320165). Can also bind sheared DNA and dodecamer DNA with a low affinity (PubMed:29320165). Interacts with mesophyll plasmodesmata to mediate its own cell-to-cell transport and potentiate RNA trafficking (By similarity). May play a role in plant defense signaling (By similarity).[UniProtKB:Q0JHU5][UniProtKB:Q9ZT47]<ref>PMID:29320165</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhuyan~~, A K~~]]	+	[[Category: Bhuyan AK]]
-	[[Category: Sashi, P]]	+	[[Category: Sashi P]]
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Structure from cyana 2 1~~]]	+

OCA at 09:25, 21 October 2020

OCA — Wed, 21 Oct 2020 09:25:08 GMT

←Older revision		Revision as of 09:25, 21 October 2020
Line 1:		Line 1:

	==Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter==		==Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter==
-	<StructureSection load='5yq3' size='340' side='right' caption='[[5yq3]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='5yq3' size='340' side='right'caption='[[5yq3]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yq3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ3 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YQ3 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yq3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YQ3 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq3 OCA], [http://pdbe.org/5yq3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yq3 RCSB], [http://www.ebi.ac.uk/pdbsum/5yq3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq3 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq3 OCA], [http://pdbe.org/5yq3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yq3 RCSB], [http://www.ebi.ac.uk/pdbsum/5yq3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq3 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bhuyan, A K]]		[[Category: Bhuyan, A K]]
	[[Category: Sashi, P]]		[[Category: Sashi, P]]
	[[Category: Plant protein]]		[[Category: Plant protein]]
	[[Category: Structure from cyana 2 1]]		[[Category: Structure from cyana 2 1]]

OCA at 05:42, 18 April 2018

OCA — Wed, 18 Apr 2018 05:42:09 GMT

←Older revision		Revision as of 05:42, 18 April 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5yq3 is ~~ON HOLD~~	+	==Solution NMR Structure and Backbone Dynamics of the Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, A Putative mRNA Transporter==
		+	<StructureSection load='5yq3' size='340' side='right' caption='[[5yq3]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5yq3]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YQ3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YQ3 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yq3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yq3 OCA], [http://pdbe.org/5yq3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yq3 RCSB], [http://www.ebi.ac.uk/pdbsum/5yq3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yq3 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although RNA-binding proteins in plant phloem are believed to perform long-distance systemic transport of RNA in the phloem conduit, the structure of none of them is known. Arabidopsis thaliana phloem protein 16-1 (AtPP16-1) is such a putative mRNA transporter whose structure and backbone dynamics have been studied at pH 4.1 and 25 degrees C by high-resolution nuclear magnetic resonance spectroscopy. Results obtained using basic optical spectroscopic tools show that the protein is unstable with little secondary structure near the physiological pH of the phloem sap. Fluorescence-monitored titrations reveal that AtPP16-1 binds not only A. thaliana RNA (Kdiss approximately 67 nM) but also sheared DNA and model dodecamer DNA, though the affinity for DNA is approximately 15-fold lower. In the solution structure of the protein, secondary structural elements are formed by residues 3-9 (beta1), 56-62 (beta2), 133-135 (beta3), and 96-110 (alpha-helix). Most of the rest of the chain segments are disordered. The N-terminally disordered regions (residues 10-55) form a small lobe, which conjoins the rest of the molecule via a deep and large irregular cleft that could have functional implications. The average order parameter extracted by model-free analysis of (15)N relaxation and {(1)H}-(15)N heteronuclear NOE data is 0.66, suggesting less restricted backbone motion. The average conformational entropy of the backbone NH vectors is -0.31 cal mol(-1) K(-1). These results also suggest structural disorder in AtPP16-1.

-	~~Authors~~:	+	Solution NMR Structure and Backbone Dynamics of Partially Disordered Arabidopsis thaliana Phloem Protein 16-1, a Putative mRNA Transporter.,Sashi P, Singarapu KK, Bhuyan AK Biochemistry. 2018 Feb 13;57(6):912-924. doi: 10.1021/acs.biochem.7b01071. Epub, 2018 Jan 26. PMID:29320165<ref>PMID:29320165</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5yq3" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Bhuyan, A K]]
		+	[[Category: Sashi, P]]
		+	[[Category: Plant protein]]
		+	[[Category: Structure from cyana 2 1]]

OCA: Protected "5yq3" [edit=sysop:move=sysop]

OCA — Wed, 15 Nov 2017 06:15:22 GMT

Protected "5yq3" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 15 November 2017

OCA: New page: '''Unreleased structure''' The entry 5yq3 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Nov 2017 06:15:20 GMT

New page: '''Unreleased structure''' The entry 5yq3 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5yq3 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]