5yy2 - Revision history

OCA at 10:25, 27 March 2024

OCA — Wed, 27 Mar 2024 10:25:47 GMT

←Older revision		Revision as of 10:25, 27 March 2024
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	<StructureSection load='5yy2' size='340' side='right'caption='[[5yy2]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='5yy2' size='340' side='right'caption='[[5yy2]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yy2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YY2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YY2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yy2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_nidulans_FGSC_A4 Aspergillus nidulans FGSC A4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YY2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YY2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.91Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yy2 OCA], [~~http~~://pdbe.org/5yy2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yy2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yy2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yy2 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yy2 OCA], [https://pdbe.org/5yy2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yy2 RCSB], [https://www.ebi.ac.uk/pdbsum/5yy2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yy2 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/ASQI_EMENI ASQI_EMENI] Cyclopenase; part of the gene cluster that mediates the biosynthesis of the aspoquinolone mycotoxins (PubMed:25251934, PubMed:30026518). Within the pathway, the cyclopenase asqI catalyzes the conversion of 4'-methoxycyclopenin into 4'-methoxyviridicatin (PubMed:30026518). Cyclopenin can also be converted into viridicatin by asqI (PubMed:30026518). The first step of the pathway is catalyzed by the nonribosomal pepdide synthetase asqK that condenses anthranilic acid and O-methyl-L-tyrosine to produce 4'-methoxycyclopeptin. 4'-methoxycyclopeptin is then converted to 4'-methoxydehydrocyclopeptin by the ketoglutarate-dependent dioxygenase asqJ. AsqJ also converts its first product 4'-methoxydehydrocyclopeptin to 4'-methoxycyclopenin. The following conversion of 4'-methoxycyclopenin into 4'-methoxyviridicatin is catalyzed by the cyclopenase asqI. 4'-methoxyviridicatin is the precursor of quinolone natural products, and is further converted to quinolinone B. The prenyltransferase asqH1 then catalyzes the canonical Friedel-Crafts alkylation of quinolinone B with dimethylallyl cation to yield dimethylallyl quinolone, which is subjected to FAD-dependent dehydrogenation by the FAD-linked oxidoreductase asqF to yield conjugated aryl diene. The delta(3') double bond then serves as the site of the second alkylation with DMAPP catalyzed by the prenyltransferase asqH2 to yield a carbenium ion intermediate, which can be attacked by H(2)O to yield a styrenyl quinolone containing a C3'-hydroxyprenyl chain. The FAD-dependent monooxygenase asqG performs epoxidation of the terminal C7'-C8' olefin. Finally, after dehydratation of the epoxide at C3 by asqC, the quinolone epoxide rearrangement protein asqO catalyzes an enzymatic 3-exo-tet cyclization to yield the cyclopropyl-THF ring system in aspoquinolone (Probable).<ref>PMID:25251934</ref> <ref>PMID:30026518</ref>
-	~~The 6,6-quinolone scaffolds on which viridicatin-type fungal alkaloids are built are frequently found in metabolites that display useful biological activities~~. ~~Here we report in vitro and computational analyses leading to~~ the ~~discovery~~ of ~~a hemocyanin-like protein AsqI from~~ the ~~Aspergillus nidulans~~ aspoquinolone ~~biosynthetic~~ pathway ~~that forms viridicatins via a~~ conversion of ~~the cyclopenin~~-~~type 6,7~~-~~bicyclic system~~ into ~~the~~ viridicatin-~~type 6,6~~-~~bicyclic core through elimination~~ of ~~carbon dioxide and methylamine through methyl isocyanate~~.	+
-		+
-	~~Enzymatic one~~-~~step ring contraction for~~ quinolone ~~biosynthesis~~.,~~Kishimoto S, Hara K~~, ~~Hashimoto~~ H~~, Hirayama Y, Champagne PA, Houk KN, Tang Y, Watanabe K Nat Commun. 2018 Jul 19;9~~(1)~~:2826~~. ~~doi: 10~~.~~1038/s41467~~-~~018~~-~~05221~~-5. ~~PMID:30026518~~<ref>PMID:~~30026518~~</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5yy2" style="background-color~~:~~#fffaf0;">~~</~~div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aspergillus nidulans FGSC A4]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hara, K]]	+	[[Category: Hara K]]
-	[[Category: Hashimoto, H]]	+	[[Category: Hashimoto H]]
-	[[Category: Kishimoto, S]]	+	[[Category: Kishimoto S]]
-	[[Category: Watanabe, K]]	+	[[Category: Watanabe K]]
-	~~[[Category: Aspoquinolone biosynthesis]]~~	+
-	~~[[Category: Hemocyanin like protein]]~~	+
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Secondary metabolism~~]]	+

OCA at 09:30, 21 October 2020

OCA — Wed, 21 Oct 2020 09:30:51 GMT

←Older revision		Revision as of 09:30, 21 October 2020
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	==Crystal structure of AsqI with Zn==		==Crystal structure of AsqI with Zn==
-	<StructureSection load='5yy2' size='340' side='right' caption='[[5yy2]], [[Resolution\|resolution]] 2.91Å' scene=''>	+	<StructureSection load='5yy2' size='340' side='right'caption='[[5yy2]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yy2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YY2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YY2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yy2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YY2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YY2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yy2 OCA], [http://pdbe.org/5yy2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yy2 RCSB], [http://www.ebi.ac.uk/pdbsum/5yy2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yy2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yy2 OCA], [http://pdbe.org/5yy2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yy2 RCSB], [http://www.ebi.ac.uk/pdbsum/5yy2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yy2 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Hara, K]]		[[Category: Hara, K]]
	[[Category: Hashimoto, H]]		[[Category: Hashimoto, H]]

OCA at 18:39, 1 August 2018

OCA — Wed, 01 Aug 2018 18:39:43 GMT

←Older revision		Revision as of 18:39, 1 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5yy2 is ~~ON HOLD until Dec 07 2019~~	+	==Crystal structure of AsqI with Zn==
		+	<StructureSection load='5yy2' size='340' side='right' caption='[[5yy2]], [[Resolution\|resolution]] 2.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5yy2]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YY2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YY2 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yy2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yy2 OCA], [http://pdbe.org/5yy2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yy2 RCSB], [http://www.ebi.ac.uk/pdbsum/5yy2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yy2 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The 6,6-quinolone scaffolds on which viridicatin-type fungal alkaloids are built are frequently found in metabolites that display useful biological activities. Here we report in vitro and computational analyses leading to the discovery of a hemocyanin-like protein AsqI from the Aspergillus nidulans aspoquinolone biosynthetic pathway that forms viridicatins via a conversion of the cyclopenin-type 6,7-bicyclic system into the viridicatin-type 6,6-bicyclic core through elimination of carbon dioxide and methylamine through methyl isocyanate.

-	~~Authors~~:	+	Enzymatic one-step ring contraction for quinolone biosynthesis.,Kishimoto S, Hara K, Hashimoto H, Hirayama Y, Champagne PA, Houk KN, Tang Y, Watanabe K Nat Commun. 2018 Jul 19;9(1):2826. doi: 10.1038/s41467-018-05221-5. PMID:30026518<ref>PMID:30026518</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5yy2" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hara, K]]
		+	[[Category: Hashimoto, H]]
		+	[[Category: Kishimoto, S]]
		+	[[Category: Watanabe, K]]
		+	[[Category: Aspoquinolone biosynthesis]]
		+	[[Category: Hemocyanin like protein]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Secondary metabolism]]

OCA at 04:50, 20 December 2017

OCA — Wed, 20 Dec 2017 04:50:10 GMT

←Older revision		Revision as of 04:50, 20 December 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5yy2 is ON HOLD	+	The entry 5yy2 is ON HOLD until Dec 07 2019

	Authors:		Authors:

OCA: Protected "5yy2" [edit=sysop:move=sysop]

OCA — Wed, 13 Dec 2017 05:49:21 GMT

Protected "5yy2" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:49, 13 December 2017

OCA: New page: '''Unreleased structure''' The entry 5yy2 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Dec 2017 05:49:20 GMT

New page: '''Unreleased structure''' The entry 5yy2 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5yy2 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]