5yyq - Revision history

OCA at 08:40, 22 November 2023

OCA — Wed, 22 Nov 2023 08:40:32 GMT

←Older revision		Revision as of 08:40, 22 November 2023
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	<StructureSection load='5yyq' size='340' side='right'caption='[[5yyq]], [[Resolution\|resolution]] 1.07Å' scene=''>		<StructureSection load='5yyq' size='340' side='right'caption='[[5yyq]], [[Resolution\|resolution]] 1.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yyq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YYQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5YYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yyq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thaumatococcus_daniellii Thaumatococcus daniellii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YYQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5YYQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~, ~~<scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.07Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[3al7\|3al7]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5yyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yyq OCA], [~~http~~://pdbe.org/5yyq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5yyq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5yyq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5yyq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5yyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yyq OCA], [https://pdbe.org/5yyq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5yyq RCSB], [https://www.ebi.ac.uk/pdbsum/5yyq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5yyq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/THM1_THADA THM1_THADA] Taste-modifying protein; intensely sweet-tasting. It is 100000 times sweeter than sucrose on a molar basis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kigo, S]]	+	[[Category: Thaumatococcus daniellii]]
-	[[Category: Kitabatake, N]]	+	[[Category: Kigo S]]
-	[[Category: Masuda, T]]	+	[[Category: Kitabatake N]]
-	[[Category: Mikami, B]]	+	[[Category: Masuda T]]
-	[[Category: Mitsumoto, M]]	+	[[Category: Mikami B]]
-	[[Category: Ohta, K]]	+	[[Category: Mitsumoto M]]
-	[[Category: Suzuki, M]]	+	[[Category: Ohta K]]
-	[[Category: Tani, F]]	+	[[Category: Suzuki M]]
-	~~[[Category: Plant protein]]~~	+	[[Category: Tani F]]
-	~~[[Category: Sweet-tasting protein~~]]	+

OCA at 09:31, 21 October 2020

OCA — Wed, 21 Oct 2020 09:31:31 GMT

←Older revision		Revision as of 09:31, 21 October 2020
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	==Structure K78A thaumatin==		==Structure K78A thaumatin==
-	<StructureSection load='5yyq' size='340' side='right' caption='[[5yyq]], [[Resolution\|resolution]] 1.07Å' scene=''>	+	<StructureSection load='5yyq' size='340' side='right'caption='[[5yyq]], [[Resolution\|resolution]] 1.07Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5yyq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YYQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5YYQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5yyq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YYQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5YYQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3al7\|3al7]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3al7\|3al7]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5yyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yyq OCA], [http://pdbe.org/5yyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yyq RCSB], [http://www.ebi.ac.uk/pdbsum/5yyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yyq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5yyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yyq OCA], [http://pdbe.org/5yyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yyq RCSB], [http://www.ebi.ac.uk/pdbsum/5yyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yyq ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kigo, S]]		[[Category: Kigo, S]]
	[[Category: Kitabatake, N]]		[[Category: Kitabatake, N]]

OCA at 07:36, 21 March 2018

OCA — Wed, 21 Mar 2018 07:36:11 GMT

←Older revision		Revision as of 07:36, 21 March 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5yyq is ~~ON HOLD~~	+	==Structure K78A thaumatin==
		+	<StructureSection load='5yyq' size='340' side='right' caption='[[5yyq]], [[Resolution\|resolution]] 1.07Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5yyq]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YYQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YYQ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[3al7\|3al7]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yyq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yyq OCA], [http://pdbe.org/5yyq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yyq RCSB], [http://www.ebi.ac.uk/pdbsum/5yyq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yyq ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Thaumatin, an intensely sweet-tasting protein, elicits sweet taste with a threshold of only 50 nM. Previous studies from our laboratory suggested that the complex model between the T1R2-T1R3 sweet receptor and thaumatin depends critically on the complementarity of electrostatic potentials. In order to further validate this model, we focused on three lysine residues (Lys78, Lys106, and Lys137), which were expected to be part of the interaction sites. Three thaumatin mutants (K78A, K106A, and K137A) were prepared and their threshold values of sweetness were examined. The results showed that the sweetness of K106A was reduced by about three times and those of K78A and K137A were reduced by about five times when compared to wild-type thaumatin. The three-dimensional structures of these mutants were also determined by X-ray crystallographic analyses at atomic resolutions. The overall structures of mutant proteins were similar to that of wild-type but the electrostatic potentials around the mutated sites became more negative. Since the three lysine residues are located in 20-40 A apart each other on the surface of thaumatin molecule, these results suggest the positive charges on the surface of thaumatin play a crucial role in the interaction with the sweet receptor, and are consistent with a large surface is required for interaction with the sweet receptor, as proposed by the multipoint interaction model named wedge model.

-	~~Authors~~:	+	Positive Charges on the Surface of Thaumatin Are Crucial for the Multi-Point Interaction with the Sweet Receptor.,Masuda T, Kigo S, Mitsumoto M, Ohta K, Suzuki M, Mikami B, Kitabatake N, Tani F Front Mol Biosci. 2018 Feb 13;5:10. doi: 10.3389/fmolb.2018.00010. eCollection, 2018. PMID:29487853<ref>PMID:29487853</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5yyq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Kigo, S]]
		+	[[Category: Kitabatake, N]]
		+	[[Category: Masuda, T]]
		+	[[Category: Mikami, B]]
		+	[[Category: Mitsumoto, M]]
		+	[[Category: Ohta, K]]
		+	[[Category: Suzuki, M]]
		+	[[Category: Tani, F]]
		+	[[Category: Plant protein]]
		+	[[Category: Sweet-tasting protein]]

OCA: Protected "5yyq" [edit=sysop:move=sysop]

OCA — Wed, 20 Dec 2017 04:51:40 GMT

Protected "5yyq" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:51, 20 December 2017

OCA: New page: '''Unreleased structure''' The entry 5yyq is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 20 Dec 2017 04:51:39 GMT

New page: '''Unreleased structure''' The entry 5yyq is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5yyq is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]