5z0y - Revision history

OCA at 08:43, 22 November 2023

OCA — Wed, 22 Nov 2023 08:43:40 GMT

←Older revision		Revision as of 08:43, 22 November 2023
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	<StructureSection load='5z0y' size='340' side='right'caption='[[5z0y]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='5z0y' size='340' side='right'caption='[[5z0y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z0y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z0Y OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5Z0Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z0y]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Komagataella_phaffii_CBS_7435 Komagataella phaffii CBS 7435]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z0Y OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z0Y FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/Glycine_hydroxymethyltransferase Glycine hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.1 2.1.2.1]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5z0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z0y OCA], [~~http~~://pdbe.org/5z0y PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z0y RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z0y PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z0y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z0y OCA], [https://pdbe.org/5z0y PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z0y RCSB], [https://www.ebi.ac.uk/pdbsum/5z0y PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z0y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/F2QZA4_KOMPC F2QZA4_KOMPC]] Interconversion of serine and glycine.[RuleBase:RU000585]	+	[https://www.uniprot.org/uniprot/F2QZA4_KOMPC F2QZA4_KOMPC] Interconversion of serine and glycine.[RuleBase:RU000585]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	==See Also==		==See Also==
-	*[[Serine hydroxymethyltransferase\|Serine hydroxymethyltransferase]]	+	*[[Serine hydroxymethyltransferase 3D structures\|Serine hydroxymethyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glycine hydroxymethyltransferase~~]]	+	[[Category: Komagataella phaffii CBS 7435]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, Z]]	+	[[Category: Chen Z]]
-	[[Category: Zhang, M]]	+	[[Category: Zhang M]]
-	~~[[Category: Cytoplasm serine hydroxymethyltransferase]]~~	+
-	~~[[Category: Pichia pastori]]~~	+
-	~~[[Category: Shmt]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:32, 21 October 2020

OCA — Wed, 21 Oct 2020 09:32:52 GMT

←Older revision		Revision as of 09:32, 21 October 2020
Line 1:		Line 1:

	==Crystallization and structure determination of cytoplasm serine hydroxymethyltransferase (SHMT) from Pichia pastoris==		==Crystallization and structure determination of cytoplasm serine hydroxymethyltransferase (SHMT) from Pichia pastoris==
-	<StructureSection load='5z0y' size='340' side='right' caption='[[5z0y]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='5z0y' size='340' side='right'caption='[[5z0y]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z0y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z0Y OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z0Y FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z0y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z0Y OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z0Y FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycine_hydroxymethyltransferase Glycine hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.1 2.1.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycine_hydroxymethyltransferase Glycine hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.1 2.1.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z0y OCA], [http://pdbe.org/5z0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z0y RCSB], [http://www.ebi.ac.uk/pdbsum/5z0y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z0y ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z0y OCA], [http://pdbe.org/5z0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z0y RCSB], [http://www.ebi.ac.uk/pdbsum/5z0y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z0y ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 5z0y" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5z0y" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Serine hydroxymethyltransferase\|Serine hydroxymethyltransferase]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Glycine hydroxymethyltransferase]]		[[Category: Glycine hydroxymethyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Chen, Z]]		[[Category: Chen, Z]]
	[[Category: Zhang, M]]		[[Category: Zhang, M]]

OCA at 06:28, 14 March 2018

OCA — Wed, 14 Mar 2018 06:28:14 GMT

←Older revision		Revision as of 06:28, 14 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5z0y is ~~ON HOLD until Paper~~ Publication	+	==Crystallization and structure determination of cytoplasm serine hydroxymethyltransferase (SHMT) from Pichia pastoris==
		+	<StructureSection load='5z0y' size='340' side='right' caption='[[5z0y]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z0y]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z0Y OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z0Y FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glycine_hydroxymethyltransferase Glycine hydroxymethyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.1 2.1.2.1] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z0y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z0y OCA], [http://pdbe.org/5z0y PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z0y RCSB], [http://www.ebi.ac.uk/pdbsum/5z0y PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z0y ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/F2QZA4_KOMPC F2QZA4_KOMPC]] Interconversion of serine and glycine.[RuleBase:RU000585]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Serine hydroxymethyltransferase (SHMT) catalyzes the interconversion of serine and glycine, which is crucial for one carbon metabolism. Here, we report the first crystal structure of cytoplasmic SHMT from Pichia pastoris (pcSHMT) diffracted to 2.5A resolution in space group C2221. PcSHMT was a contaminant with our target protein expressed in Pichia pastoris and confirmed by mass spectrometry. The overall structure of pcSHMT is similar to Human mitochondrial SHMT and different to E. coli SHMT. Interestingly, the oligomerization of pcSHMT expressed in eukaryotic or prokaryotic system differs significantly and is regulated by pyridoxal-5'-phosphate. Our results revealed a close evolutionary relationship between Pichia pastoris and Human mitochondria.

-	~~Authors: Chen~~, Z.Z., ~~Zhang, M~~.F.	+	Structure and function of cytoplasmic serine hydroxymethyltransferase from Pichia pastoris.,Zhang M, Wu W, Chen Z Biochem Biophys Res Commun. 2018 Feb 5;496(2):753-757. doi:, 10.1016/j.bbrc.2018.01.084. Epub 2018 Jan 12. PMID:29339156<ref>PMID:29339156</ref>

-	~~Description: Crystallization and structure determination~~ of ~~cytoplasm serine~~ hydroxymethyltransferase ~~(SHMT) from Pichia pastoris~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Zhang, M.F]]	+	<div class="pdbe-citations 5z0y" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Chen, Z.Z~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glycine hydroxymethyltransferase]]
		+	[[Category: Chen, Z]]
		+	[[Category: Zhang, M]]
		+	[[Category: Cytoplasm serine hydroxymethyltransferase]]
		+	[[Category: Pichia pastori]]
		+	[[Category: Shmt]]
		+	[[Category: Transferase]]

OCA at 07:04, 10 January 2018

OCA — Wed, 10 Jan 2018 07:04:23 GMT

←Older revision		Revision as of 07:04, 10 January 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z0y is ON HOLD	+	The entry 5z0y is ON HOLD until Paper Publication

	Authors: Chen, Z.Z., Zhang, M.F.		Authors: Chen, Z.Z., Zhang, M.F.

OCA: Protected "5z0y" [edit=sysop:move=sysop]

OCA — Wed, 27 Dec 2017 07:14:58 GMT

Protected "5z0y" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:14, 27 December 2017

OCA: New page: '''Unreleased structure''' The entry 5z0y is ON HOLD Authors: Chen, Z.Z., Zhang, M.F. Description: Crystallization and structure determination of cytoplasm serine hydroxymethyltransfer...

OCA — Wed, 27 Dec 2017 07:14:57 GMT

New page: '''Unreleased structure''' The entry 5z0y is ON HOLD Authors: Chen, Z.Z., Zhang, M.F. Description: Crystallization and structure determination of cytoplasm serine hydroxymethyltransfer...

New page

'''Unreleased structure'''

The entry 5z0y is ON HOLD

Authors: Chen, Z.Z., Zhang, M.F.

Description: Crystallization and structure determination of cytoplasm serine hydroxymethyltransferase (SHMT) from Pichia pastoris
[[Category: Unreleased Structures]]
[[Category: Zhang, M.F]]
[[Category: Chen, Z.Z]]