5z3r - Revision history

OCA at 08:44, 22 November 2023

OCA — Wed, 22 Nov 2023 08:44:45 GMT

←Older revision		Revision as of 08:44, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5z3r' size='340' side='right'caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='5z3r' size='340' side='right'caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_25795 Atcc 25795~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_neoaurum Mycolicibacterium neoaurum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~ksi (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1795 ATCC 25795])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.42Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [~~http~~://pdbe.org/5z3r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [https://pdbe.org/5z3r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [https://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/V5XH97_MYCNE V5XH97_MYCNE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 25:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 25795]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cheng, X Y~~]]	+	[[Category: Mycolicibacterium neoaurum]]
-	[[Category: ~~Huang, Y Q~~]]	+	[[Category: Cheng XY]]
-	[[Category: ~~Peng, F~~]]	+	[[Category: Huang YQ]]
-	[[Category: ~~Su, Z D]]~~	+	[[Category: Peng F]]
-	~~[[Category: Yang,~~ F]]	+	[[Category: Su ZD]]
-	[[Category: ~~Biosynthetic protein~~]]	+	[[Category: Yang F]]
-	[[Category: ~~Ketosteroid isomerase~~]]	+

OCA at 06:24, 21 August 2019

OCA — Wed, 21 Aug 2019 06:24:51 GMT

←Older revision		Revision as of 06:24, 21 August 2019
Line 1:		Line 1:

	==Crystal Structure of Delta 5-3-Ketosteroid Isomerase from Mycobacterium sp.==		==Crystal Structure of Delta 5-3-Ketosteroid Isomerase from Mycobacterium sp.==
-	<StructureSection load='5z3r' size='340' side='right' caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>	+	<StructureSection load='5z3r' size='340' side='right'caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25795 Atcc 25795]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>		<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25795 Atcc 25795]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>
Line 7:		Line 7:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [http://pdbe.org/5z3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [http://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [http://pdbe.org/5z3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [http://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Protein engineering based on structure homology holds the potential to engineer steroid-transforming enzymes on demand. Based on the genome sequencing analysis of industrial Mycobacterium strain HGMS2 to produce 4-androstene-3,17-dione (4-AD), three hypothetical proteins were predicted as putative Delta(5)-3-ketosteroid isomerases (KSIs) to catalyze an intramolecular proton transfer involving the transformation of 5-androstene-3,17-dione (5-AD) into 4-AD, which were defined as mKSI228, mKSI291 and mKSI753. Activity assays indicated that mKSI228 and mKSI291 exhibited weak activity, as low as 0.7% and 1.5%, respectively, of a well-studied and highly active KSI from Pseudomonas putida KSI (pKSI), while mKSI753 had no activity similar to Mycobacterium tuberculosis KSI (mtKSI). Although the 3D structures of the putative mKSIs were homologous to pKSI, their amino acid sequences were significantly different from those of pKSI and tKSI. Thus, by use of these two KSIs as homology models, we were able to convert the low-active mKSI291 into a high-active active KSI by site-directed mutagenesis. On the other hand, an X-ray crystallographic structure of mKSI291 identified a water molecule in its active site. This unique water molecule might function as a bridge to connect Ser-OH, Tyr57-OH and C3O of the intermediate form a hydrogen-bonding network that was responsible for its weak activity, compared with that of mtKSI. Our results not only demonstrated the use of a protein engineering approach to understanding KSI catalytic mechanism, but also provided an example for engineering the catalytic active sites and gaining a functional enzyme based on homologous structures.
		+
		+	Structure-based reconstruction of a Mycobacterium hypothetical protein into an active Delta(5)-3-ketosteroid isomerase.,Peng F, Cheng X, Wang H, Song S, Chen T, Li X, He Y, Huang Y, Liu S, Yang F, Su Z Biochim Biophys Acta Proteins Proteom. 2019 Sep;1867(9):821-830. doi:, 10.1016/j.bbapap.2019.06.008. Epub 2019 Jun 19. PMID:31226491<ref>PMID:31226491</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5z3r" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Ketosteroid Isomerase\|Ketosteroid Isomerase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Atcc 25795]]		[[Category: Atcc 25795]]
		+	[[Category: Large Structures]]
	[[Category: Cheng, X Y]]		[[Category: Cheng, X Y]]
	[[Category: Huang, Y Q]]		[[Category: Huang, Y Q]]

OCA at 07:43, 23 May 2018

OCA — Wed, 23 May 2018 07:43:08 GMT

←Older revision		Revision as of 07:43, 23 May 2018
Line 3:		Line 3:
	<StructureSection load='5z3r' size='340' side='right' caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>		<StructureSection load='5z3r' size='340' side='right' caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25795 Atcc 25795]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [http://pdbe.org/5z3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [http://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ksi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1795 ATCC 25795])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [http://pdbe.org/5z3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [http://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 25795]]
	[[Category: Cheng, X Y]]		[[Category: Cheng, X Y]]
	[[Category: Huang, Y Q]]		[[Category: Huang, Y Q]]

OCA at 05:56, 31 January 2018

OCA — Wed, 31 Jan 2018 05:56:01 GMT

←Older revision		Revision as of 05:56, 31 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5z3r ~~is ON HOLD~~	+	==Crystal Structure of Delta 5-3-Ketosteroid Isomerase from Mycobacterium sp.==
-		+	<StructureSection load='5z3r' size='340' side='right' caption='[[5z3r]], [[Resolution\|resolution]] 2.42Å' scene=''>
-	~~Authors~~: ~~Cheng, X~~.Y., ~~Peng, F~~., ~~Yang, F~~., ~~Huang, Y~~.Q., ~~Su, Z~~.D.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5z3r]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z3R FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of Delta 5~~-~~3-Ketosteroid Isomerase from Mycobacterium sp~~.	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z3r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z3r OCA], [http://pdbe.org/5z3r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z3r RCSB], [http://www.ebi.ac.uk/pdbsum/5z3r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z3r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: Su, ~~Z.D~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Cheng, X Y]]
		+	[[Category: Huang, Y Q]]
	[[Category: Peng, F]]		[[Category: Peng, F]]
-	[[Category: ~~Huang~~, ~~Y.Q~~]]	+	[[Category: Su, Z D]]
	[[Category: Yang, F]]		[[Category: Yang, F]]
-	[[Category: ~~Cheng, X.Y~~]]	+	[[Category: Biosynthetic protein]]
		+	[[Category: Ketosteroid isomerase]]

OCA: Protected "5z3r" [edit=sysop:move=sysop]

OCA — Wed, 17 Jan 2018 05:33:10 GMT

Protected "5z3r" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:33, 17 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z3r is ON HOLD Authors: Cheng, X.Y., Peng, F., Yang, F., Huang, Y.Q., Su, Z.D. Description: Crystal Structure of Delta 5-3-Ketosteroid Isomerase f...

OCA — Wed, 17 Jan 2018 05:33:08 GMT

New page: '''Unreleased structure''' The entry 5z3r is ON HOLD Authors: Cheng, X.Y., Peng, F., Yang, F., Huang, Y.Q., Su, Z.D. Description: Crystal Structure of Delta 5-3-Ketosteroid Isomerase f...

New page

'''Unreleased structure'''

The entry 5z3r is ON HOLD

Authors: Cheng, X.Y., Peng, F., Yang, F., Huang, Y.Q., Su, Z.D.

Description: Crystal Structure of Delta 5-3-Ketosteroid Isomerase from Mycobacterium sp.
[[Category: Unreleased Structures]]
[[Category: Su, Z.D]]
[[Category: Peng, F]]
[[Category: Huang, Y.Q]]
[[Category: Yang, F]]
[[Category: Cheng, X.Y]]