5z68 - Revision history

OCA at 21:14, 28 October 2020

OCA — Wed, 28 Oct 2020 21:14:56 GMT

←Older revision		Revision as of 21:14, 28 October 2020
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	==Structure of the recombination mediator protein RecF-ATP in RecFOR pathway==		==Structure of the recombination mediator protein RecF-ATP in RecFOR pathway==
-	<StructureSection load='5z68' size='340' side='right' caption='[[5z68]], [[Resolution\|resolution]] 3.00Å' scene=''>	+	<StructureSection load='5z68' size='340' side='right'caption='[[5z68]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z68 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z68 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z68 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z68 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z68 OCA], [http://pdbe.org/5z68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z68 RCSB], [http://www.ebi.ac.uk/pdbsum/5z68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z68 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z68 OCA], [http://pdbe.org/5z68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z68 RCSB], [http://www.ebi.ac.uk/pdbsum/5z68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z68 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Liu, Y P]]		[[Category: Liu, Y P]]
	[[Category: Tang, Q]]		[[Category: Tang, Q]]

OCA at 05:42, 18 April 2018

OCA — Wed, 18 Apr 2018 05:42:29 GMT

←Older revision		Revision as of 05:42, 18 April 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5z68 is ~~ON HOLD until Paper~~ Publication	+	==Structure of the recombination mediator protein RecF-ATP in RecFOR pathway==
		+	<StructureSection load='5z68' size='340' side='right' caption='[[5z68]], [[Resolution\|resolution]] 3.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z68]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z68 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z68 OCA], [http://pdbe.org/5z68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z68 RCSB], [http://www.ebi.ac.uk/pdbsum/5z68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z68 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RECF_CALS4 RECF_CALS4]] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	RecF is a principal member of the RecF pathway. It interacts with RecO and RecR to initiate homologous recombination by loading RecA recombinases on single-stranded DNA and displacing single-stranded DNA-binding proteins. As an ATP-binding cassette ATPase, RecF exhibits ATP-dependent dimerization and structural homology with Rad50 and SMC proteins. However, the mechanism and action pattern of RecF ATP-dependent dimerization remains unclear. Here, We determined three crystal structures of TTERecF, TTERecF-ATP and TTERecF-ATPS from Thermoanaerobacter tengcongensis that reveal a novel ATP-driven RecF dimerization. RecF contains a positively charged tunnel on its dimer interface that is essential to ATP binding. Our structural and biochemical data indicate that the Walker A motif serves as a switch and plays a key role in ATP binding and RecF dimerization. Furthermore, Biolayer interferometry assay results showed that the TTERecF interacted with ATP and formed a dimer, displaying a higher affinity for DNA than that of the TTERecF monomer. Overall, our results provide a solid structural basis for understanding the process of RecF binding with ATP and the functional mechanism of ATP-dependent RecF dimerization.

-	~~Authors: Tang~~, Q., Liu, ~~Y.-P.~~, ~~Yan~~, X.-X.	+	ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.,Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496<ref>PMID:29391496</ref>

-	~~Description: Structure~~ of the ~~recombination mediator protein RecF~~-~~ATP in RecFOR pathway~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5z68" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Liu, Y P]]
	[[Category: Tang, Q]]		[[Category: Tang, Q]]
-	[[Category: Yan, X.-X]]	+	[[Category: Yan, X X]]
-	[[Category: ~~Liu, Y.-P~~]]	+	[[Category: Dna binding protein]]
		+	[[Category: Dna repair]]
		+	[[Category: Rad50]]
		+	[[Category: Recf]]
		+	[[Category: Recfor]]
		+	[[Category: Recombination mediator]]

OCA at 12:14, 11 April 2018

OCA — Wed, 11 Apr 2018 12:14:36 GMT

←Older revision		Revision as of 12:14, 11 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z68 is ON HOLD	+	The entry 5z68 is ON HOLD until Paper Publication

	Authors: Tang, Q., Liu, Y.-P., Yan, X.-X.		Authors: Tang, Q., Liu, Y.-P., Yan, X.-X.

OCA: Protected "5z68" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:40:43 GMT

Protected "5z68" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:40, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z68 is ON HOLD Authors: Tang, Q., Liu, Y.-P., Yan, X.-X. Description: Structure of the recombination mediator protein RecF-ATP in RecFOR pathway [...

OCA — Wed, 31 Jan 2018 04:40:42 GMT

New page: '''Unreleased structure''' The entry 5z68 is ON HOLD Authors: Tang, Q., Liu, Y.-P., Yan, X.-X. Description: Structure of the recombination mediator protein RecF-ATP in RecFOR pathway [...

New page

'''Unreleased structure'''

The entry 5z68 is ON HOLD

Authors: Tang, Q., Liu, Y.-P., Yan, X.-X.

Description: Structure of the recombination mediator protein RecF-ATP in RecFOR pathway
[[Category: Unreleased Structures]]
[[Category: Tang, Q]]
[[Category: Yan, X.-X]]
[[Category: Liu, Y.-P]]