5z6h - Revision history

OCA at 08:46, 22 November 2023

OCA — Wed, 22 Nov 2023 08:46:31 GMT

←Older revision		Revision as of 08:46, 22 November 2023
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	<StructureSection load='5z6h' size='340' side='right'caption='[[5z6h]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='5z6h' size='340' side='right'caption='[[5z6h]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z6h]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter~~ cloacae~~" (jordan 1890) bergey et al. 1923~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z6H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z6h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae Enterobacter cloacae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z6H FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Related_structure~~\|~~Related:]]</b></td><td class="sblockDat">[[5z66\|5z66~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>treA, SAMEA2273171_02252 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~550~~ "~~Aerobacter cloacae~~" ~~(Jordan 1890) Bergey et al. 1923])</td~~><~~/tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6h OCA], [https://pdbe.org/5z6h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z6h RCSB], [https://www.ebi.ac.uk/pdbsum/5z6h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6h ProSAT]</span></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Alpha,alpha-trehalase Alpha,alpha-trehalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.28 3.2.1.28]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6h OCA], [~~http~~://pdbe.org/5z6h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z6h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z6h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6h ProSAT]</span></td></tr>	+
	</table>		</table>
-	== Function ==
-	[[http://www.uniprot.org/uniprot/A0A156C5X3_ENTCL A0A156C5X3_ENTCL]] Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system.[HAMAP-Rule:MF_01060]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Alpha,alpha-trehalase~~]]	+	[[Category: Enterobacter cloacae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Adhav~~, A S~~]]	+	[[Category: Adhav AS]]
-	[[Category: Gayathri, P]]	+	[[Category: Gayathri P]]
-	[[Category: Harne~~, S R~~]]	+	[[Category: Harne SR]]
-	[[Category: Joshi~~, R S]]~~	+	[[Category: Joshi RS]]
-	~~[[Category: Apo structure]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Trehalase from insect gut bacterium~~]]	+

OCA at 07:24, 23 May 2019

OCA — Thu, 23 May 2019 07:24:17 GMT

←Older revision		Revision as of 07:24, 23 May 2019
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	==Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form==		==Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form==
-	<StructureSection load='5z6h' size='340' side='right' caption='[[5z6h]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='5z6h' size='340' side='right'caption='[[5z6h]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[5z6h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6H FirstGlance]. <br>		<table><tr><td colspan='2'>[[5z6h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6H FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Alpha,alpha-trehalase]]		[[Category: Alpha,alpha-trehalase]]
		+	[[Category: Large Structures]]
	[[Category: Adhav, A S]]		[[Category: Adhav, A S]]
	[[Category: Gayathri, P]]		[[Category: Gayathri, P]]

OCA at 15:52, 27 February 2019

OCA — Wed, 27 Feb 2019 15:52:20 GMT

←Older revision		Revision as of 15:52, 27 February 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 5z6h is ~~ON HOLD until Paper~~ Publication	+	==Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form==
		+	<StructureSection load='5z6h' size='340' side='right' caption='[[5z6h]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z6h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"aerobacter_cloacae"_(jordan_1890)_bergey_et_al._1923 "aerobacter cloacae" (jordan 1890) bergey et al. 1923]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6H FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5z66\|5z66]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">treA, SAMEA2273171_02252 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=550 "Aerobacter cloacae" (Jordan 1890) Bergey et al. 1923])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha,alpha-trehalase Alpha,alpha-trehalase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.28 3.2.1.28] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z6h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6h OCA], [http://pdbe.org/5z6h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z6h RCSB], [http://www.ebi.ac.uk/pdbsum/5z6h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6h ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/A0A156C5X3_ENTCL A0A156C5X3_ENTCL]] Provides the cells with the ability to utilize trehalose at high osmolarity by splitting it into glucose molecules that can subsequently be taken up by the phosphotransferase-mediated uptake system.[HAMAP-Rule:MF_01060]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Energy metabolism in the diamondback moth Plutella xylostella is facilitated by trehalase, an enzyme which assists in trehalose hydrolysis, from the predominant gut bacterium Enterobacter cloacae. We report the biochemical and structural characterization of recombinant trehalase from E. cloacae (Px_EclTre). Px_EclTre showed KM of 1.47 (+/-0.05) mm, kcat of 6254.72 min(-1) and Vmax 0.2 (+/-0.002) mm.min(-1) at 55 degrees C and acidic pH. Crystal structures of Px_EclTre were determined in the ligand-free form and bound to the inhibitor Validoxylamine A. The crystal structure of the ligand-free form, unavailable until now for any other bacterial trehalases, enabled us to delineate the conformational changes accompanying ligand binding in trehalases. Multiple salt bridges were identified that potentially facilitated closure of a hood over the substrate-binding site. A cluster of five tryptophans lined the -1 substrate-binding subsite, interacted with crucial active site residues and contributed to both trehalase activity and stability. The importance of these residues in enzyme activity was further validated by mutagenesis studies. Many of these identified residues form part of signature motifs and other conserved sequences in trehalases. The structure analysis thus led to the assignment of the functional role to these conserved residues. This information can be further explored for the design of effective inhibitors against trehalases.

-	~~Authors: Harne, S.R~~., Adhav, A.S., ~~Joshi~~, ~~R.S.~~, Gayathri, P.	+	Mechanistic insights into enzymatic catalysis by trehalase from the insect gut endosymbiont Enterobacter cloacae.,Adhav A, Harne S, Bhide A, Giri A, Gayathri P, Joshi R FEBS J. 2019 Jan 18. doi: 10.1111/febs.14760. PMID:30657252<ref>PMID:30657252</ref>

-	~~Description: Structure~~ of ~~periplasmic trehalase from Diamondback moth gut bacteria in~~ the ~~apo form~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Harne~~, S.R]]	+	<div class="pdbe-citations 5z6h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alpha,alpha-trehalase]]
		+	[[Category: Adhav, A S]]
	[[Category: Gayathri, P]]		[[Category: Gayathri, P]]
-	[[Category: ~~Adhav~~, A.S]]	+	[[Category: Harne, S R]]
-	[[Category: Joshi, R.S]]	+	[[Category: Joshi, R S]]
		+	[[Category: Apo structure]]
		+	[[Category: Hydrolase]]
		+	[[Category: Trehalase from insect gut bacterium]]

OCA at 06:15, 14 November 2018

OCA — Wed, 14 Nov 2018 06:15:22 GMT

←Older revision		Revision as of 06:15, 14 November 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z6h is ON HOLD	+	The entry 5z6h is ON HOLD until Paper Publication

	Authors: Harne, S.R., Adhav, A.S., Joshi, R.S., Gayathri, P.		Authors: Harne, S.R., Adhav, A.S., Joshi, R.S., Gayathri, P.
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	Description: Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form		Description: Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Adhav, A.S]]
	[[Category: Harne, S.R]]		[[Category: Harne, S.R]]
	[[Category: Gayathri, P]]		[[Category: Gayathri, P]]
		+	[[Category: Adhav, A.S]]
	[[Category: Joshi, R.S]]		[[Category: Joshi, R.S]]

OCA: Protected "5z6h" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:41:15 GMT

Protected "5z6h" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:41, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z6h is ON HOLD Authors: Harne, S.R., Adhav, A.S., Joshi, R.S., Gayathri, P. Description: Structure of periplasmic trehalase from Diamondback moth ...

OCA — Wed, 31 Jan 2018 04:41:13 GMT

New page: '''Unreleased structure''' The entry 5z6h is ON HOLD Authors: Harne, S.R., Adhav, A.S., Joshi, R.S., Gayathri, P. Description: Structure of periplasmic trehalase from Diamondback moth ...

New page

'''Unreleased structure'''

The entry 5z6h is ON HOLD

Authors: Harne, S.R., Adhav, A.S., Joshi, R.S., Gayathri, P.

Description: Structure of periplasmic trehalase from Diamondback moth gut bacteria in the apo form
[[Category: Unreleased Structures]]
[[Category: Adhav, A.S]]
[[Category: Harne, S.R]]
[[Category: Gayathri, P]]
[[Category: Joshi, R.S]]