5z6p - Revision history

OCA at 08:46, 22 November 2023

OCA — Wed, 22 Nov 2023 08:46:37 GMT

←Older revision		Revision as of 08:46, 22 November 2023
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	==The crystal structure of an agarase, AgWH50C==		==The crystal structure of an agarase, AgWH50C==
-	<StructureSection load='5z6p' size='340' side='right' caption='[[5z6p]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='5z6p' size='340' side='right'caption='[[5z6p]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Agarivorans_gilvus Agarivorans gilvus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z6P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Agarivorans_gilvus Agarivorans gilvus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z6P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [~~http~~://pdbe.org/5z6p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.061Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [https://pdbe.org/5z6p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [https://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A023LLI1_9ALTE A0A023LLI1_9ALTE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Agarivorans gilvus]]		[[Category: Agarivorans gilvus]]
-	[[Category: Li, Y]]	+	[[Category: Large Structures]]
-	[[Category: Mao, X]]	+	[[Category: Li Y]]
-	[[Category: Zhang, J]]	+	[[Category: Mao X]]
-	[[Category: Zhang, L]]	+	[[Category: Zhang J]]
-	[[Category: Zhang, P]]	+	[[Category: Zhang L]]
-	[[Category: Zhou, J]]	+	[[Category: Zhang P]]
-	~~[[Category: Agarase activity]]~~	+	[[Category: Zhou J]]
-	~~[[Category: Glucoside hydrolase 50 family]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 08:17, 6 March 2019

OCA — Wed, 06 Mar 2019 08:17:41 GMT

←Older revision		Revision as of 08:17, 6 March 2019
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	==The crystal structure of an agarase, AgWH50C==		==The crystal structure of an agarase, AgWH50C==
-	<StructureSection load='5z6p' size='340' side='right' caption='[[5z6p]], [[Resolution\|resolution]] 2.06Å' scene=''>	+	<StructureSection load='5z6p' size='340' side='right' caption='[[5z6p]], [[Resolution\|resolution]] 2.06Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Agarivorans_gilvus Agarivorans gilvus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6P FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [http://pdbe.org/5z6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [http://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [http://pdbe.org/5z6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [http://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Agarivorans gilvus]]
	[[Category: Li, Y]]		[[Category: Li, Y]]
	[[Category: Mao, X]]		[[Category: Mao, X]]

OCA at 06:07, 2 January 2019

OCA — Wed, 02 Jan 2019 06:07:32 GMT

←Older revision		Revision as of 06:07, 2 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 5z6p is ~~ON HOLD~~	+	==The crystal structure of an agarase, AgWH50C==
		+	<StructureSection load='5z6p' size='340' side='right' caption='[[5z6p]], [[Resolution\|resolution]] 2.06Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z6p]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z6P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z6P FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z6p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z6p OCA], [http://pdbe.org/5z6p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z6p RCSB], [http://www.ebi.ac.uk/pdbsum/5z6p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z6p ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	AgWH50C, an exo-beta-agarase of GH50 isolated from Agarivorans gilvus WH0801, plays a key role in the enzymatic production of neoagarobiose, which has great application prospect in the cosmetics and pharmaceutical industry. In contrast, the poor thermostability becomes the main obstructive factor of glycoside hydrolase (GH) family 50 agarases, including AgWH50C. Herein, based on the AgWH50C crystal structure, we designed several mutants by a multiple cross-linked rational design protocol used thermostability predicting softwares ETSS, PoPMuSiC, and HotMuSiC. To our surprise, the mutant K621F increased its relative activity by as much as 45% and the optimal temperature increased to 38 degrees C compared to that of wild-type, AgWH50C (30 degrees C). The thermostability of K621F also exhibited a substantial improvement. Considering that the gelling temperature of the agarose is higher than 35 degrees C, K621F can be used to hydrolyze agarose for neoagarobiose production.

-	~~Authors: Zhang~~, P~~.J.~~, Zhang, L.J., ~~Zhang~~, J.R., ~~Li, Y~~.	+	Structure-based design of agarase AgWH50C from Agarivorans gilvus WH0801 to enhance thermostability.,Zhang P, Zhang J, Zhang L, Sun J, Li Y, Wu L, Zhou J, Xue C, Mao X Appl Microbiol Biotechnol. 2018 Dec 6. pii: 10.1007/s00253-018-9540-1. doi:, 10.1007/s00253-018-9540-1. PMID:30523371<ref>PMID:30523371</ref>

-	~~Description: The crystal structure of an agarase~~, ~~AgWH50C~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Zhang, L.J]]~~	+	<div class="pdbe-citations 5z6p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Li, Y]]		[[Category: Li, Y]]
-	[[Category: Zhang, J.R]]	+	[[Category: Mao, X]]
-	[[Category: Zhang, P.J]]	+	[[Category: Zhang, J]]
		+	[[Category: Zhang, L]]
		+	[[Category: Zhang, P]]
		+	[[Category: Zhou, J]]
		+	[[Category: Agarase activity]]
		+	[[Category: Glucoside hydrolase 50 family]]
		+	[[Category: Hydrolase]]

OCA at 04:59, 7 February 2018

OCA — Wed, 07 Feb 2018 04:59:13 GMT

←Older revision		Revision as of 04:59, 7 February 2018
Line 5:		Line 5:
	Authors: Zhang, P.J., Zhang, L.J., Zhang, J.R., Li, Y.		Authors: Zhang, P.J., Zhang, L.J., Zhang, J.R., Li, Y.

-	Description: The crystal structure of an agarase, AgWH50C.	+	Description: The crystal structure of an agarase, AgWH50C
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Zhang, L.J]]		[[Category: Zhang, L.J]]

OCA: Protected "5z6p" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:41:53 GMT

Protected "5z6p" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:41, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z6p is ON HOLD Authors: Zhang, P.J., Zhang, L.J., Zhang, J.R., Li, Y. Description: The crystal structure of an agarase, AgWH50C. [[Category: Unrel...

OCA — Wed, 31 Jan 2018 04:41:52 GMT

New page: '''Unreleased structure''' The entry 5z6p is ON HOLD Authors: Zhang, P.J., Zhang, L.J., Zhang, J.R., Li, Y. Description: The crystal structure of an agarase, AgWH50C. [[Category: Unrel...

New page

'''Unreleased structure'''

The entry 5z6p is ON HOLD

Authors: Zhang, P.J., Zhang, L.J., Zhang, J.R., Li, Y.

Description: The crystal structure of an agarase, AgWH50C.
[[Category: Unreleased Structures]]
[[Category: Zhang, L.J]]
[[Category: Li, Y]]
[[Category: Zhang, J.R]]
[[Category: Zhang, P.J]]