5z70 - Revision history

OCA at 08:47, 22 November 2023

OCA — Wed, 22 Nov 2023 08:47:17 GMT

←Older revision		Revision as of 08:47, 22 November 2023
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	<StructureSection load='5z70' size='340' side='right'caption='[[5z70]], [[Resolution\|resolution]] 2.91Å' scene=''>		<StructureSection load='5z70' size='340' side='right'caption='[[5z70]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z70]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Stenotrophomonas_sp.~~_kctc_12332~~ Stenotrophomonas sp. ~~kctc~~ 12332]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z70 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Stenotrophomonas_sp._KCTC_12332 Stenotrophomonas sp. KCTC 12332]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z70 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~AXG53_12705 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=1793721 Stenotrophomonas sp. KCTC 12332])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.91Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z70 OCA], [~~http~~://pdbe.org/5z70 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z70 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z70 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z70 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z70 OCA], [https://pdbe.org/5z70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z70 RCSB], [https://www.ebi.ac.uk/pdbsum/5z70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z70 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A126NKL7_9GAMM A0A126NKL7_9GAMM]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Stenotrophomonas sp. ~~kctc~~ 12332]]	+	[[Category: Stenotrophomonas sp. KCTC 12332]]
-	[[Category: Park~~, A K]]~~	+	[[Category: Park AK]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Oleate hydratase~~]]	+

OCA at 15:49, 28 August 2019

OCA — Wed, 28 Aug 2019 15:49:59 GMT

←Older revision		Revision as of 15:49, 28 August 2019
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-	==Crystal ~~strucure~~ of oleate hydratase from Stenotrophomonas sp. KCTC 12332==	+	==Crystal structure of oleate hydratase from Stenotrophomonas sp. KCTC 12332==
-	<StructureSection load='5z70' size='340' side='right' caption='[[5z70]], [[Resolution\|resolution]] 2.91Å' scene=''>	+	<StructureSection load='5z70' size='340' side='right'caption='[[5z70]], [[Resolution\|resolution]] 2.91Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z70]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z70 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z70]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Stenotrophomonas_sp._kctc_12332 Stenotrophomonas sp. kctc 12332]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z70 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z70 OCA], [http://pdbe.org/5z70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z70 RCSB], [http://www.ebi.ac.uk/pdbsum/5z70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z70 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AXG53_12705 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1793721 Stenotrophomonas sp. KCTC 12332])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z70 OCA], [http://pdbe.org/5z70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z70 RCSB], [http://www.ebi.ac.uk/pdbsum/5z70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z70 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Stenotrophomonas sp. kctc 12332]]
	[[Category: Park, A K]]		[[Category: Park, A K]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Oleate hydratase]]		[[Category: Oleate hydratase]]

OCA at 10:02, 5 September 2018

OCA — Wed, 05 Sep 2018 10:02:02 GMT

←Older revision		Revision as of 10:02, 5 September 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5z70 is ~~ON HOLD until Paper~~ Publication	+	==Crystal strucure of oleate hydratase from Stenotrophomonas sp. KCTC 12332==
		+	<StructureSection load='5z70' size='340' side='right' caption='[[5z70]], [[Resolution\|resolution]] 2.91Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z70]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z70 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z70 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z70 OCA], [http://pdbe.org/5z70 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z70 RCSB], [http://www.ebi.ac.uk/pdbsum/5z70 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z70 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Unsaturated fatty acids are toxic to various bacteria, causing their death or growth inhibition. To prevent this toxicity, unsaturated fatty acids should be converted into saturated fatty acids via hydrogenation reaction, which is the complete reduction of double bonds on the carbon chain. In a recent report, we observed that Stenotrophomonas sp. KCTC 12332 exhibited a high biotransformation activity of oleic acid (OA) in 10-hydroxystearic acid and identified the gene encoding oleate hydratase (OhySt) by complete genomic analysis. In the present study, to further investigate the structural features of OhySt, the recombinant protein was expressed in Escherichia coli, and then purified and crystallized. Biochemical assay showed that OhySt produces 10-hydroxystearic acid in a flavin adenosine dinucleotide (FAD)-dependent manner, indicating that it requires FAD as a cofactor. The OhySt structure, which is determined in its apo state, allows for a structural comparison with the previously reported FAD bound structure of oleate hydratase. The comparison of structures indicates remarkable conformational change of the loop region surrounding the FAD molecule upon binding of FAD. This change forces one of the important catalytic residues into position for catalysis.

-	~~Authors~~:	+	Crystal structure of oleate hydratase from Stenotrophomonas sp. KCTC 12332 reveals conformational plasticity surrounding the FAD binding site.,Park AK, Lee GH, Kim DW, Jang EH, Kwon HT, Chi YM Biochem Biophys Res Commun. 2018 May 23;499(4):772-776. doi:, 10.1016/j.bbrc.2018.03.220. Epub 2018 Apr 3. PMID:29608896<ref>PMID:29608896</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5z70" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Park, A K]]
		+	[[Category: Hydrolase]]
		+	[[Category: Oleate hydratase]]

OCA at 05:29, 4 July 2018

OCA — Wed, 04 Jul 2018 05:29:16 GMT

←Older revision		Revision as of 05:29, 4 July 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z70 is ON HOLD	+	The entry 5z70 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 03:48, 16 May 2018

OCA — Wed, 16 May 2018 03:48:00 GMT

←Older revision		Revision as of 03:48, 16 May 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z70 is ON HOLD ~~until Paper Publication~~	+	The entry 5z70 is ON HOLD

	Authors:		Authors:

OCA at 12:14, 11 April 2018

OCA — Wed, 11 Apr 2018 12:14:42 GMT

←Older revision		Revision as of 12:14, 11 April 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z70 is ON HOLD	+	The entry 5z70 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5z70" [edit=sysop:move=sysop]

OCA — Wed, 31 Jan 2018 04:43:17 GMT

Protected "5z70" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:43, 31 January 2018

OCA: New page: '''Unreleased structure''' The entry 5z70 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 31 Jan 2018 04:43:16 GMT

New page: '''Unreleased structure''' The entry 5z70 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5z70 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]