5z8q - Revision history

OCA at 10:35, 14 June 2023

OCA — Wed, 14 Jun 2023 10:35:11 GMT

←Older revision		Revision as of 10:35, 14 June 2023
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	==Solution structure of the SBDalpha domain of yeast Ssa1==		==Solution structure of the SBDalpha domain of yeast Ssa1==
-	<StructureSection load='5z8q' size='340' side='right' caption='[[5z8q~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5z8q' size='340' side='right'caption='[[5z8q]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z8q]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Baker's_yeast Baker's yeast~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z8q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z8Q FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSA1, YAL005C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8q OCA], [https://pdbe.org/5z8q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z8q RCSB], [https://www.ebi.ac.uk/pdbsum/5z8q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8q ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8q OCA], [~~http~~://pdbe.org/5z8q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z8q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z8q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8q ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HSP71_YEAST HSP71_YEAST]] May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.<ref>PMID:12761219</ref>	+	[https://www.uniprot.org/uniprot/HSP71_YEAST HSP71_YEAST] May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.<ref>PMID:12761219</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 18:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 5z8q" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5z8q" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Heat Shock Protein structures\|Heat Shock Protein structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Baker's yeast~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Gong, W~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
-	[[Category: ~~Hu,~~ W]]	+	[[Category: Gong W]]
-	[[Category: ~~Perrett, S~~]]	+	[[Category: Hu W]]
-	[[Category: ~~Chaperone]]~~	+	[[Category: Perrett S]]
-	~~[[Category: Sbdalpha domain]]~~	+
-	~~[[Category: Yeast ssa1~~]]	+

OCA at 08:27, 10 October 2018

OCA — Wed, 10 Oct 2018 08:27:21 GMT

←Older revision		Revision as of 08:27, 10 October 2018
Line 3:		Line 3:
	<StructureSection load='5z8q' size='340' side='right' caption='[[5z8q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='5z8q' size='340' side='right' caption='[[5z8q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z8q]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z8Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z8q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z8Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8q OCA], [http://pdbe.org/5z8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z8q RCSB], [http://www.ebi.ac.uk/pdbsum/5z8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8q ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSA1, YAL005C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8q OCA], [http://pdbe.org/5z8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z8q RCSB], [http://www.ebi.ac.uk/pdbsum/5z8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/HSP71_YEAST HSP71_YEAST]] May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.<ref>PMID:12761219</ref>		[[http://www.uniprot.org/uniprot/HSP71_YEAST HSP71_YEAST]] May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.<ref>PMID:12761219</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The allosteric coupling of the highly conserved nucleotide- and substrate-binding domains of Hsp70 has been studied intensively. In contrast, the role of the disordered, highly variable C-terminal region of Hsp70 remains unclear. In many eukaryotic Hsp70s, the extreme C-terminal EEVD motif binds to tetratricopeptide-repeat domains of Hsp70 co-chaperones. Here, we discovered that the TVEEVD sequence of Saccharomyces cerevisiae cytoplasmic Hsp70 (Ssa1) functions as a SUMO-interacting motif. A second C-terminal motif of ~15 amino acids between the alpha-helical lid and the extreme C terminus, previously identified in bacterial and eukaryotic organellar Hsp70s, is known to enhance chaperone function by transiently interacting with folding clients. Using structural analysis, interaction studies, fibril formation assays, and in vivo functional assays, we investigated the individual contributions of the alpha-helical bundle and the C-terminal disordered region of Ssa1 in the inhibition of fibril formation of the prion protein Ure2. Our results revealed that although the alpha-helical bundle of the Ssa1 substrate-binding domain (SBDalpha) does not directly bind to Ure2, the SBDalpha enhances the ability of Hsp70 to inhibit fibril formation. We found that a 20-residue C-terminal motif in Ssa1, containing GGAP and GGAP-like tetra-peptide repeats, can directly bind to Ure2, the Hsp40 co-chaperone Ydj1, and alpha-synuclein, but not to the SUMO-like protein SMT3 or BSA. Deletion or substitution of the Ssa1 GGAP motif impaired yeast cell tolerance to temperature and cell wall damage stress. This study highlights that the C-terminal GGAP motif of Hsp70 is important for substrate recognition and mediation of the heat shock response.
		+
		+	The C-terminal GGAP motif of Hsp70 mediates substrate recognition and stress response in yeast.,Gong W, Hu W, Xu L, Wu H, Wu S, Zhang H, Wang J, Jones GW, Perrett S J Biol Chem. 2018 Sep 18. pii: RA118.002691. doi: 10.1074/jbc.RA118.002691. PMID:30228181<ref>PMID:30228181</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5z8q" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Baker's yeast]]
	[[Category: Gong, W]]		[[Category: Gong, W]]
	[[Category: Hu, W]]		[[Category: Hu, W]]

OCA at 07:55, 26 September 2018

OCA — Wed, 26 Sep 2018 07:55:31 GMT

←Older revision		Revision as of 07:55, 26 September 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5z8q ~~is ON HOLD until Paper Publication~~	+	==Solution structure of the SBDalpha domain of yeast Ssa1==
-		+	<StructureSection load='5z8q' size='340' side='right' caption='[[5z8q]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
-	~~Authors~~: ~~Weibin~~, G., ~~Wanhui~~, H., ~~Sarah~~, P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5z8q]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z8Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z8Q FirstGlance]. <br>
-	~~Description~~: ~~Solution structure~~ of the ~~SBDalpha domain~~ of ~~yeast Ssa1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z8q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z8q OCA], [http://pdbe.org/5z8q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z8q RCSB], [http://www.ebi.ac.uk/pdbsum/5z8q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z8q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Sarah~~, P]]	+	== Function ==
-	[[Category: ~~Wanhui~~, H]]	+	[[http://www.uniprot.org/uniprot/HSP71_YEAST HSP71_YEAST]] May play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. A functional difference between SSA1 and SSA2 proteins is expected. SSA1 can participate in the ATP-dependent disassembly of clathrin-coated vesicles.<ref>PMID:12761219</ref>
-	[[Category: ~~Weibin, G~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Gong, W]]
		+	[[Category: Hu, W]]
		+	[[Category: Perrett, S]]
		+	[[Category: Chaperone]]
		+	[[Category: Sbdalpha domain]]
		+	[[Category: Yeast ssa1]]

OCA at 12:14, 11 April 2018

OCA — Wed, 11 Apr 2018 12:14:56 GMT

←Older revision		Revision as of 12:14, 11 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z8q is ON HOLD	+	The entry 5z8q is ON HOLD until Paper Publication

	Authors: Weibin, G., Wanhui, H., Sarah, P.		Authors: Weibin, G., Wanhui, H., Sarah, P.

OCA: Protected "5z8q" [edit=sysop:move=sysop]

OCA — Wed, 07 Feb 2018 05:04:04 GMT

Protected "5z8q" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:04, 7 February 2018

OCA: New page: '''Unreleased structure''' The entry 5z8q is ON HOLD Authors: Weibin, G., Wanhui, H., Sarah, P. Description: Solution structure of the SBDalpha domain of yeast Ssa1 [[Category: Unrelea...

OCA — Wed, 07 Feb 2018 05:04:03 GMT

New page: '''Unreleased structure''' The entry 5z8q is ON HOLD Authors: Weibin, G., Wanhui, H., Sarah, P. Description: Solution structure of the SBDalpha domain of yeast Ssa1 [[Category: Unrelea...

New page

'''Unreleased structure'''

The entry 5z8q is ON HOLD

Authors: Weibin, G., Wanhui, H., Sarah, P.

Description: Solution structure of the SBDalpha domain of yeast Ssa1
[[Category: Unreleased Structures]]
[[Category: Sarah, P]]
[[Category: Wanhui, H]]
[[Category: Weibin, G]]