5z9g - Revision history

OCA at 08:50, 22 November 2023

OCA — Wed, 22 Nov 2023 08:50:14 GMT

←Older revision		Revision as of 08:50, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5z9g' size='340' side='right'caption='[[5z9g]], [[Resolution\|resolution]] 1.49Å' scene=''>		<StructureSection load='5z9g' size='340' side='right'caption='[[5z9g]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z9g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z9G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5Z9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z9g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z9G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Z9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5z9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z9g OCA], [~~http~~://pdbe.org/5z9g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5z9g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5z9g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5z9g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.49Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5z9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z9g OCA], [https://pdbe.org/5z9g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5z9g RCSB], [https://www.ebi.ac.uk/pdbsum/5z9g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5z9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/KAI2_ARATH KAI2_ARATH]] Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.<ref>PMID:20864454</ref> <ref>PMID:22357928</ref> <ref>PMID:23142794</ref> <ref>PMID:23301669</ref>	+	[https://www.uniprot.org/uniprot/KAI2_ARATH KAI2_ARATH] Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.<ref>PMID:20864454</ref> <ref>PMID:22357928</ref> <ref>PMID:23142794</ref> <ref>PMID:23301669</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arabidopsis thaliana]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cha~~, J S~~]]	+	[[Category: Cha JS]]
-	[[Category: Cho~~, H S~~]]	+	[[Category: Cho HS]]
-	[[Category: Kim~~, K L~~]]	+	[[Category: Kim KL]]
-	[[Category: Soh~~, M S]]~~	+	[[Category: Soh MS]]
-	~~[[Category: Alpha/beta hydrolase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 21:16, 28 October 2020

OCA — Wed, 28 Oct 2020 21:16:46 GMT

←Older revision		Revision as of 21:16, 28 October 2020
Line 1:		Line 1:

	==Crystal structure of KAI2==		==Crystal structure of KAI2==
-	<StructureSection load='5z9g' size='340' side='right' caption='[[5z9g]], [[Resolution\|resolution]] 1.49Å' scene=''>	+	<StructureSection load='5z9g' size='340' side='right'caption='[[5z9g]], [[Resolution\|resolution]] 1.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5z9g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z9G OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5Z9G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5z9g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z9G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z9G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5z9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z9g OCA], [http://pdbe.org/5z9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z9g RCSB], [http://www.ebi.ac.uk/pdbsum/5z9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z9g ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z9g OCA], [http://pdbe.org/5z9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z9g RCSB], [http://www.ebi.ac.uk/pdbsum/5z9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z9g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 21:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Cha, J S]]		[[Category: Cha, J S]]
	[[Category: Cho, H S]]		[[Category: Cho, H S]]

OCA at 06:00, 22 August 2018

OCA — Wed, 22 Aug 2018 06:00:45 GMT

←Older revision		Revision as of 06:00, 22 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5z9g is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of KAI2==
		+	<StructureSection load='5z9g' size='340' side='right' caption='[[5z9g]], [[Resolution\|resolution]] 1.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5z9g]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z9G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z9G FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z9g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z9g OCA], [http://pdbe.org/5z9g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z9g RCSB], [http://www.ebi.ac.uk/pdbsum/5z9g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z9g ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/KAI2_ARATH KAI2_ARATH]] Involved in seed germination and seedling development. Essential for plant responses to karrikins, a class of butenolide compounds, structurally similar to strigolactones, released from burning vegetation that stimulate seed germination and enhance seedling photomorphogenesis. KAI2 is not required for strigolactone-mediated responses, but MAX2 is necessary for responses to karrikins and strigolactones.<ref>PMID:20864454</ref> <ref>PMID:22357928</ref> <ref>PMID:23142794</ref> <ref>PMID:23301669</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A smoke-derived compound, karrikin (KAR), and an endogenous but as yet unidentified KARRIKIN INSENSITIVE2 (KAI2) ligand (KL) have been identified as chemical cues in higher plants that impact on multiple aspects of growth and development. Genetic screening of light-signaling mutants in Arabidopsis thaliana has identified a mutant designated as ply2 (pleiotropic long hypocotyl2) that has pleiotropic light-response defects. In this study, we used positional cloning to identify the molecular lesion of ply2 as a missense mutation of KAI2/HYPOSENSITIVE TO LIGHT, which causes a single amino acid substitution, Ala219Val. Physiological analysis and genetic epistasis analysis with the KL-signaling components MORE AXILLARY GROWTH2 (MAX2) and SUPPRESSOR OF MAX2 1 suggested that the pleiotropic phenotypes of the ply2 mutant can be ascribed to a defect in KL-signaling. Molecular and biochemical analyses revealed that the mutant KAI2ply2 protein is impaired in its ligand-binding activity. In support of this conclusion, X-ray crystallography studies suggested that the KAI2ply2 mutation not only results in a narrowed entrance gate for the ligand but also alters the structural flexibility of the helical lid domains. We discuss the structural implications of the Ala219 residue with regard to ligand-specific binding and signaling of KAI2, together with potential functions of KL-signaling in the context of the light-regulatory network in Arabidopsis thaliana.

-	~~Authors:~~ Kim, K~~.L.~~, ~~Cha~~, J~~.S.~~, ~~Soh~~, M.S., Cho, H.S.	+	A missense allele of KARRIKIN-INSENSITIVE2 impairs ligand-binding and downstream signaling in Arabidopsis thaliana.,Lee I, Kim K, Lee S, Lee S, Hwang E, Shin K, Kim D, Choi J, Choi H, Cha JS, Kim H, Lee RA, Jeong S, Kim J, Kim Y, Nam HG, Park SK, Cho HS, Soh MS J Exp Bot. 2018 Jun 27;69(15):3609-3623. doi: 10.1093/jxb/ery164. PMID:29722815<ref>PMID:29722815</ref>

-	~~Description: Crystal structure~~ of ~~KAI2~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Soh~~, M.S]]	+	<div class="pdbe-citations 5z9g" style="background-color:#fffaf0;"></div>
-	[[Category: Kim, K.L]]	+	== References ==
-	[[Category: ~~Cha~~, J.S]]	+	<references/>
-	[[Category: ~~Cho, H.S~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Cha, J S]]
		+	[[Category: Cho, H S]]
		+	[[Category: Kim, K L]]
		+	[[Category: Soh, M S]]
		+	[[Category: Alpha/beta hydrolase]]
		+	[[Category: Hydrolase]]

OCA at 03:50, 20 June 2018

OCA — Wed, 20 Jun 2018 03:50:37 GMT

←Older revision		Revision as of 03:50, 20 June 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5z9g is ON HOLD	+	The entry 5z9g is ON HOLD until Paper Publication

	Authors: Kim, K.L., Cha, J.S., Soh, M.S., Cho, H.S.		Authors: Kim, K.L., Cha, J.S., Soh, M.S., Cho, H.S.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Soh, M.S]]		[[Category: Soh, M.S]]
		+	[[Category: Kim, K.L]]
	[[Category: Cha, J.S]]		[[Category: Cha, J.S]]
	[[Category: Cho, H.S]]		[[Category: Cho, H.S]]
-	[[Category: Kim, K.L]]

OCA: Protected "5z9g" [edit=sysop:move=sysop]

OCA — Thu, 15 Feb 2018 05:09:20 GMT

Protected "5z9g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:09, 15 February 2018

OCA: New page: '''Unreleased structure''' The entry 5z9g is ON HOLD Authors: Kim, K.L., Cha, J.S., Soh, M.S., Cho, H.S. Description: Crystal structure of KAI2 Category: Unreleased Structures [[Ca...

OCA — Thu, 15 Feb 2018 05:09:18 GMT

New page: '''Unreleased structure''' The entry 5z9g is ON HOLD Authors: Kim, K.L., Cha, J.S., Soh, M.S., Cho, H.S. Description: Crystal structure of KAI2 Category: Unreleased Structures [[Ca...

New page

'''Unreleased structure'''

The entry 5z9g is ON HOLD

Authors: Kim, K.L., Cha, J.S., Soh, M.S., Cho, H.S.

Description: Crystal structure of KAI2
[[Category: Unreleased Structures]]
[[Category: Soh, M.S]]
[[Category: Cha, J.S]]
[[Category: Cho, H.S]]
[[Category: Kim, K.L]]