5zfs - Revision history

OCA at 08:53, 22 November 2023

OCA — Wed, 22 Nov 2023 08:53:34 GMT

←Older revision		Revision as of 08:53, 22 November 2023
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	<StructureSection load='5zfs' size='340' side='right'caption='[[5zfs]], [[Resolution\|resolution]] 1.96Å' scene=''>		<StructureSection load='5zfs' size='340' side='right'caption='[[5zfs]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arthrobacter_globiformis Arthrobacter globiformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.96Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [~~http~~://pdbe.org/5zfs PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [https://pdbe.org/5zfs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [https://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KET3E_ARTGO KET3E_ARTGO] Catalyzes the reversible C-3 epimerization of several ketoses. Shows the highest enzymatic activity for the epimerization of L-ribulose to L-xylulose. Is also able to convert D-allulose (also known as D-psicose) to D-fructose and, to a lesser extent, L-tagatose to L-sorbose, D-ribulose to D-xylulose, L-allulose to L-fructose and D-tagatose to D-sorbose.<ref>PMID:27713017</ref> <ref>PMID:30279320</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Arthrobacter globiformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akimitsu, K]]	+	[[Category: Akimitsu K]]
-	[[Category: Gullapalli~~, P K~~]]	+	[[Category: Gullapalli PK]]
-	[[Category: Izumori, K]]	+	[[Category: Izumori K]]
-	[[Category: Kamitori, S]]	+	[[Category: Kamitori S]]
-	[[Category: Ohtani, K]]	+	[[Category: Ohtani K]]
-	[[Category: Yoshida, H]]	+	[[Category: Yoshida H]]
-	[[Category: Yoshihara, A]]	+	[[Category: Yoshihara A]]
-	~~[[Category: Epimerase]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 21:19, 28 October 2020

OCA — Wed, 28 Oct 2020 21:19:12 GMT

←Older revision		Revision as of 21:19, 28 October 2020
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	==Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose==		==Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose==
-	<StructureSection load='5zfs' size='340' side='right' caption='[[5zfs]], [[Resolution\|resolution]] 1.96Å' scene=''>	+	<StructureSection load='5zfs' size='340' side='right'caption='[[5zfs]], [[Resolution\|resolution]] 1.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [http://pdbe.org/5zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [http://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [http://pdbe.org/5zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [http://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Akimitsu, K]]		[[Category: Akimitsu, K]]
	[[Category: Gullapalli, P K]]		[[Category: Gullapalli, P K]]

OCA at 05:47, 24 October 2018

OCA — Wed, 24 Oct 2018 05:47:15 GMT

←Older revision		Revision as of 05:47, 24 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5zfs is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose==
		+	<StructureSection load='5zfs' size='340' side='right' caption='[[5zfs]], [[Resolution\|resolution]] 1.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zfs]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZFS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZFS FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zfs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zfs OCA], [http://pdbe.org/5zfs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zfs RCSB], [http://www.ebi.ac.uk/pdbsum/5zfs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zfs ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The X-ray structure of ketose 3-epimerase from Arthrobacter globiformis M30, which was previously reported to be a D-allulose 3-epimerase (AgD-AE), was determined at 1.96 A resolution. The crystal belonged to the hexagonal space group P6522, with unit-cell parameters a = b = 103.98, c = 256.53 A. The structure was solved by molecular replacement using the structure of Mesorhizobium loti L-ribulose 3-epimerase (MlL-RE), which has 41% sequence identity, as a search model. A hexagonal crystal contained two molecules in the asymmetric unit, and AgD-AE formed a homotetramer with twofold symmetry. The overall structure of AgD-AE was more similar to that of MlL-RE than to the known structures of D-psicose (alternative name D-allulose) 3-epimerases (D-PEs or D-AEs), although AgD-AE and MlL-RE have different substrate specificities. Both AgD-AE and MlL-RE have long helices in the C-terminal region that would contribute to the stability of the homotetramer. AgD-AE showed higher enzymatic activity for L-ribulose than D-allulose; however, AgD-AE is stable and is a unique useful enzyme for the production of D-allulose from D-fructose.

-	~~Authors: Yoshida~~, H., Yoshihara, A., Gullapalli~~, P.K.~~, Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S.	+	X-ray structure of Arthrobacter globiformis M30 ketose 3-epimerase for the production of D-allulose from D-fructose.,Yoshida H, Yoshihara A, Gullapalli PK, Ohtani K, Akimitsu K, Izumori K, Kamitori S Acta Crystallogr F Struct Biol Commun. 2018 Oct 1;74(Pt 10):669-676. doi:, 10.1107/S2053230X18011706. Epub 2018 Sep 21. PMID:30279320<ref>PMID:30279320</ref>

-	~~Description: Crystal structure~~ of ~~Arthrobacter globiformis M30 sugar epimerase which can produce D~~-~~allulose from D~~-~~fructose~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Yoshihara, A]]~~	+	<div class="pdbe-citations 5zfs" style="background-color:#fffaf0;"></div>
-	~~[[Category: Ohtani, K]]~~	+	== References ==
-	~~[[Category: Yoshida, H]]~~	+	<references/>
-	~~[[Category: Gullapalli, P.K]]~~	+	__TOC__
		+	</StructureSection>
	[[Category: Akimitsu, K]]		[[Category: Akimitsu, K]]
-	[[Category: ~~Kamitori~~, S]]	+	[[Category: Gullapalli, P K]]
	[[Category: Izumori, K]]		[[Category: Izumori, K]]
		+	[[Category: Kamitori, S]]
		+	[[Category: Ohtani, K]]
		+	[[Category: Yoshida, H]]
		+	[[Category: Yoshihara, A]]
		+	[[Category: Epimerase]]
		+	[[Category: Isomerase]]

OCA at 05:08, 28 March 2018

OCA — Wed, 28 Mar 2018 05:08:04 GMT

←Older revision		Revision as of 05:08, 28 March 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zfs is ON HOLD	+	The entry 5zfs is ON HOLD until Paper Publication

	Authors: Yoshida, H., Yoshihara, A., Gullapalli, P.K., Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S.		Authors: Yoshida, H., Yoshihara, A., Gullapalli, P.K., Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S.

OCA: Protected "5zfs" [edit=sysop:move=sysop]

OCA — Wed, 14 Mar 2018 05:01:05 GMT

Protected "5zfs" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:01, 14 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zfs is ON HOLD Authors: Yoshida, H., Yoshihara, A., Gullapalli, P.K., Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S. Description: Crystal str...

OCA — Wed, 14 Mar 2018 05:01:03 GMT

New page: '''Unreleased structure''' The entry 5zfs is ON HOLD Authors: Yoshida, H., Yoshihara, A., Gullapalli, P.K., Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S. Description: Crystal str...

New page

'''Unreleased structure'''

The entry 5zfs is ON HOLD

Authors: Yoshida, H., Yoshihara, A., Gullapalli, P.K., Ohtani, K., Akimitsu, K., Izumori, K., Kamitori, S.

Description: Crystal structure of Arthrobacter globiformis M30 sugar epimerase which can produce D-allulose from D-fructose
[[Category: Unreleased Structures]]
[[Category: Yoshihara, A]]
[[Category: Ohtani, K]]
[[Category: Yoshida, H]]
[[Category: Gullapalli, P.K]]
[[Category: Akimitsu, K]]
[[Category: Kamitori, S]]
[[Category: Izumori, K]]