5zha - Revision history

OCA at 08:55, 22 November 2023

OCA — Wed, 22 Nov 2023 08:55:01 GMT

←Older revision		Revision as of 08:55, 22 November 2023
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	==Structure of Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus==		==Structure of Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus==
-	<StructureSection load='5zha' size='340' side='right' caption='[[5zha]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='5zha' size='340' side='right'caption='[[5zha]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zha]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Candidatus_liberibacter_asiaticus_psy62~~ Candidatus ~~liberibacter~~ asiaticus psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZHA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zha]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus_str._psy62 Candidatus Liberibacter asiaticus str. psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.84Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLIBASIA_02120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=537021 Candidatus Liberibacter asiaticus psy62])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zha OCA], [~~http~~://pdbe.org/5zha PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zha RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zha PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zha ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zha OCA], [https://pdbe.org/5zha PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zha RCSB], [https://www.ebi.ac.uk/pdbsum/5zha PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zha ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C6XF58_LIBAP C6XF58_LIBAP]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Candidatus ~~liberibacter~~ asiaticus psy62]]	+	[[Category: Candidatus Liberibacter asiaticus str. psy62]]
-	[[Category: Dalal, V]]	+	[[Category: Large Structures]]
-	[[Category: Kumar, P]]	+	[[Category: Dalal V]]
-	[[Category: Saini, G]]	+	[[Category: Kumar P]]
-	[[Category: Sharma~~, A K~~]]	+	[[Category: Saini G]]
-	[[Category: Sharma, N]]	+	[[Category: Sharma AK]]
-	~~[[Category: Abc transporter]]~~	+	[[Category: Sharma N]]
-	~~[[Category: Metal binding protein]]~~	+
-	~~[[Category: Periplasmic metal binding protein~~]]	+

OCA at 08:27, 10 October 2018

OCA — Wed, 10 Oct 2018 08:27:30 GMT

←Older revision		Revision as of 08:27, 10 October 2018
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	<StructureSection load='5zha' size='340' side='right' caption='[[5zha]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='5zha' size='340' side='right' caption='[[5zha]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zha]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zha]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candidatus_liberibacter_asiaticus_psy62 Candidatus liberibacter asiaticus psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CLIBASIA_02120 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=537021 Candidatus Liberibacter asiaticus psy62])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zha OCA], [http://pdbe.org/5zha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zha RCSB], [http://www.ebi.ac.uk/pdbsum/5zha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zha ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zha OCA], [http://pdbe.org/5zha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zha RCSB], [http://www.ebi.ac.uk/pdbsum/5zha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zha ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candidatus liberibacter asiaticus psy62]]
	[[Category: Dalal, V]]		[[Category: Dalal, V]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]

OCA at 07:38, 3 October 2018

OCA — Wed, 03 Oct 2018 07:38:47 GMT

←Older revision		Revision as of 07:38, 3 October 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 5zha is ~~ON HOLD until Paper~~ Publication	+	==Structure of Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus==
		+	<StructureSection load='5zha' size='340' side='right' caption='[[5zha]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zha]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZHA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zha FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zha OCA], [http://pdbe.org/5zha PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zha RCSB], [http://www.ebi.ac.uk/pdbsum/5zha PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zha ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The subtle internal communications through an intricate network of interactions play a key role in metal-binding and release in periplasmic metal uptake proteins of cluster A-I family, a component of ABC transport system. These proteins have evolved different mechanisms of metal-binding and release through sequence and thereby structure-function divergence. The CLas-ZnuA2 from Candidatus Liberibacter asiaticus (CLA), in previous studies, showed a lower metal-binding affinity. The subtle communications within and between domains from crystal structure analysis revealed that protein seems to prefer a metal-free state. The unique features of CLas-ZnuA2 included a highly restrained loop L3 and presence of a proline in linker helix. In present work, S38A and Y68F mutants were studied as they play an important role during metal-binding in CLas-ZnuA2. The mutations in linker helix could not be studied as the expressed protein was not soluble and in most cases degraded with time. The crystal structure analysis of (S38A and Y68F) mutants in metal-free and metal-bound forms showed variations in interactions, an increase in number of alternate conformations and distortions in secondary structure elements, despite a similar overall structure, suggesting alterations in internal communications. The results suggested that any change in critical residues could alter the subtle internal communications and result in disturbing the fine-tuned structure required for optimal functioning.

-	~~Authors: Saini~~, G., Sharma, N., Dalal, V., ~~Kumar~~, P., Sharma~~, A~~.K.	+	The analysis of subtle internal communications through mutation studies in periplasmic metal uptake protein CLas-ZnuA2.,Saini G, Sharma N, Dalal V, Warghane A, Ghosh DK, Kumar P, Sharma AK J Struct Biol. 2018 Nov;204(2):228-239. doi: 10.1016/j.jsb.2018.08.013. Epub 2018, Aug 17. PMID:30125692<ref>PMID:30125692</ref>

-	~~Description: Structure~~ of ~~Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures]]~~	+	</div>
-	~~[[Category~~: ~~Sharma, N]]~~	+	<div class="pdbe-citations 5zha" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Dalal, V]]		[[Category: Dalal, V]]
-	[[Category: Sharma, A.K]]
-	[[Category: Saini, G]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
		+	[[Category: Saini, G]]
		+	[[Category: Sharma, A K]]
		+	[[Category: Sharma, N]]
		+	[[Category: Abc transporter]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Periplasmic metal binding protein]]

OCA at 12:17, 11 April 2018

OCA — Wed, 11 Apr 2018 12:17:39 GMT

←Older revision		Revision as of 12:17, 11 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zha is ON HOLD	+	The entry 5zha is ON HOLD until Paper Publication

	Authors: Saini, G., Sharma, N., Dalal, V., Kumar, P., Sharma, A.K.		Authors: Saini, G., Sharma, N., Dalal, V., Kumar, P., Sharma, A.K.

OCA: Protected "5zha" [edit=sysop:move=sysop]

OCA — Wed, 21 Mar 2018 05:59:43 GMT

Protected "5zha" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:59, 21 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zha is ON HOLD Authors: Saini, G., Sharma, N., Dalal, V., Kumar, P., Sharma, A.K. Description: Structure of Y68F mutant Mn-Bound periplasmic metal...

OCA — Wed, 21 Mar 2018 05:59:42 GMT

New page: '''Unreleased structure''' The entry 5zha is ON HOLD Authors: Saini, G., Sharma, N., Dalal, V., Kumar, P., Sharma, A.K. Description: Structure of Y68F mutant Mn-Bound periplasmic metal...

New page

'''Unreleased structure'''

The entry 5zha is ON HOLD

Authors: Saini, G., Sharma, N., Dalal, V., Kumar, P., Sharma, A.K.

Description: Structure of Y68F mutant Mn-Bound periplasmic metal binding protein from candidatus liberibacter asiaticus
[[Category: Unreleased Structures]]
[[Category: Sharma, N]]
[[Category: Dalal, V]]
[[Category: Sharma, A.K]]
[[Category: Saini, G]]
[[Category: Kumar, P]]