OCA at 08:55, 22 November 2023

OCA — Wed, 22 Nov 2023 08:55:54 GMT

←Older revision		Revision as of 08:55, 22 November 2023
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	==Crystal structure of OsD14 in complex with covalently bound KK094==		==Crystal structure of OsD14 in complex with covalently bound KK094==
-	<StructureSection load='5zhr' size='340' side='right'caption='[[5zhr]]' scene=''>	+	<StructureSection load='5zhr' size='340' side='right'caption='[[5zhr]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZHR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zhr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryza_sativa_Japonica_Group Oryza sativa Japonica Group]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZHR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhr OCA], [~~http~~://pdbe.org/5zhr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zhr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zhr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KOK:(2,3-dihydro-1H-indol-1-yl)(1H-1,2,3-triazol-1-yl)methanone'>KOK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhr OCA], [https://pdbe.org/5zhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zhr RCSB], [https://www.ebi.ac.uk/pdbsum/5zhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D14_ORYSJ D14_ORYSJ] Involved in strigolactone signaling pathway. May function downstream of strigolactone synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of strigolactones to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds.<ref>PMID:19542179</ref> <ref>PMID:23301669</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Strigolactones, a class of plant hormones with multiple functions, mediate plant-plant and plant-microorganism communications in the rhizosphere. In this study, we developed potent strigolactone antagonists, which covalently bind to the strigolactone receptor D14, by preparing an array of triazole urea compounds. Using yeast two-hybrid and rice-tillering assays, we identified a triazole urea compound KK094 as a potent inhibitor of strigolactone receptors. Liquid chromatography-tandem mass spectrometry analysis and X-ray crystallography revealed that KK094 was hydrolyzed by D14, and that a reaction product of this degradation covalently binds to the Ser residue of the catalytic triad of D14. Furthermore, we identified two triazole urea compounds KK052 and KK073, whose effects on D14-D53/D14-SLR1 complex formation were opposite due to the absence (KK052) or presence (KK073) of a trifluoromethyl group on their phenyl ring. These results demonstrate that triazole urea compounds are potentially powerful tools for agricultural application and may be useful for the elucidation of the complicated mechanism underlying strigolactone perception.
		+
		+	Triazole Ureas Covalently Bind to Strigolactone Receptor and Antagonize Strigolactone Responses.,Nakamura H, Hirabayashi K, Miyakawa T, Kikuzato K, Hu W, Xu Y, Jiang K, Takahashi I, Niiyama R, Dohmae N, Tanokura M, Asami T Mol Plant. 2019 Jan 7;12(1):44-58. doi: 10.1016/j.molp.2018.10.006. Epub 2018 Nov, 2. PMID:30391752<ref>PMID:30391752</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5zhr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Oryza sativa Japonica Group]]
	[[Category: Hirabayashi K]]		[[Category: Hirabayashi K]]
	[[Category: Miyakawa T]]		[[Category: Miyakawa T]]
	[[Category: Tanokura M]]		[[Category: Tanokura M]]

OCA at 21:19, 28 October 2020

OCA — Wed, 28 Oct 2020 21:19:43 GMT

←Older revision		Revision as of 21:19, 28 October 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 5zhr is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of OsD14 in complex with covalently bound KK094==
-		+	<StructureSection load='5zhr' size='340' side='right'caption='[[5zhr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZHR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZHR FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zhr OCA], [http://pdbe.org/5zhr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zhr RCSB], [http://www.ebi.ac.uk/pdbsum/5zhr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zhr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hirabayashi K]]
		+	[[Category: Miyakawa T]]
		+	[[Category: Tanokura M]]

OCA at 06:31, 2 May 2018

OCA — Wed, 02 May 2018 06:31:46 GMT

←Older revision		Revision as of 06:31, 2 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zhr is ON HOLD	+	The entry 5zhr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5zhr" [edit=sysop:move=sysop]

OCA — Wed, 21 Mar 2018 06:01:21 GMT

Protected "5zhr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:01, 21 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zhr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Mar 2018 06:01:19 GMT

New page: '''Unreleased structure''' The entry 5zhr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zhr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

5zhr - Revision history

OCA at 08:55, 22 November 2023

OCA at 21:19, 28 October 2020

OCA at 06:31, 2 May 2018

OCA: Protected "5zhr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 5zhr is ON HOLD Authors: Description: Category: Unreleased Structures