OCA at 10:24, 5 September 2018

OCA — Wed, 05 Sep 2018 10:24:07 GMT

←Older revision		Revision as of 10:24, 5 September 2018
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	<StructureSection load='5zib' size='340' side='right' caption='[[5zib]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='5zib' size='340' side='right' caption='[[5zib]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zib]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZIB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zib]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZIB FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zic\|5zic]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zic\|5zic]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MGAT5, GGNT5 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-1,6-mannosyl-glycoprotein_6-beta-N-acetylglucosaminyltransferase Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.155 2.4.1.155] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-1,6-mannosyl-glycoprotein_6-beta-N-acetylglucosaminyltransferase Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.155 2.4.1.155] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zib OCA], [http://pdbe.org/5zib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zib RCSB], [http://www.ebi.ac.uk/pdbsum/5zib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zib ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zib OCA], [http://pdbe.org/5zib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zib RCSB], [http://www.ebi.ac.uk/pdbsum/5zib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zib ProSAT]</span></td></tr>
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/MGT5A_HUMAN MGT5A_HUMAN]] Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.		[[http://www.uniprot.org/uniprot/MGT5A_HUMAN MGT5A_HUMAN]] Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	N-acetylglucosaminyltransferase-V (GnT-V) alters the structure of specific N-glycans by modifying alpha1-6-linked mannose with a beta1-6-linked N-acetylglucosamine branch. beta1-6 branch formation on cell surface receptors accelerates cancer metastasis, making GnT-V a promising target for drug development. However, the molecular basis of GnT-V's catalytic mechanism and substrate specificity are not fully understood. Here, we report crystal structures of human GnT-V luminal domain with a substrate analog. GnT-V luminal domain is composed of a GT-B fold and two accessary domains. Interestingly, two aromatic rings sandwich the alpha1-6 branch of the acceptor N-glycan and restrain the global conformation, partly explaining the fine branch specificity of GnT-V. In addition, interaction of the substrate N-glycoprotein with GnT-V likely contributes to protein-selective and site-specific glycan modification. In summary, the acceptor-GnT-V complex structure suggests a catalytic mechanism, explains the previously observed inhibition of GnT-V by branching enzyme GnT-III, and provides a basis for the rational design of drugs targeting N-glycan branching.
		+
		+	Structure and mechanism of cancer-associated N-acetylglucosaminyltransferase-V.,Nagae M, Kizuka Y, Mihara E, Kitago Y, Hanashima S, Ito Y, Takagi J, Taniguchi N, Yamaguchi Y Nat Commun. 2018 Aug 23;9(1):3380. doi: 10.1038/s41467-018-05931-w. PMID:30140003<ref>PMID:30140003</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5zib" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase]]		[[Category: Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase]]
		+	[[Category: Human]]
	[[Category: Nagae, M]]		[[Category: Nagae, M]]
	[[Category: Yamaguchi, Y]]		[[Category: Yamaguchi, Y]]
	[[Category: Glycosyltransferase cancer metastasis luminal protein]]		[[Category: Glycosyltransferase cancer metastasis luminal protein]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 18:40, 1 August 2018

OCA — Wed, 01 Aug 2018 18:40:30 GMT

←Older revision		Revision as of 18:40, 1 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zib is ~~ON HOLD~~	+	==Crystal structure of human GnT-V luminal domain in apo form==
-		+	<StructureSection load='5zib' size='340' side='right' caption='[[5zib]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5zib]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZIB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZIB FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zic\|5zic]]</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-1,6-mannosyl-glycoprotein_6-beta-N-acetylglucosaminyltransferase Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.155 2.4.1.155] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zib FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zib OCA], [http://pdbe.org/5zib PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zib RCSB], [http://www.ebi.ac.uk/pdbsum/5zib PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zib ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MGT5A_HUMAN MGT5A_HUMAN]] Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase]]
		+	[[Category: Nagae, M]]
		+	[[Category: Yamaguchi, Y]]
		+	[[Category: Glycosyltransferase cancer metastasis luminal protein]]
		+	[[Category: Sugar binding protein]]

OCA: Protected "5zib" [edit=sysop:move=sysop]

OCA — Wed, 21 Mar 2018 06:02:54 GMT

Protected "5zib" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:02, 21 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zib is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Mar 2018 06:02:53 GMT

New page: '''Unreleased structure''' The entry 5zib is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zib is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

5zib - Revision history

OCA at 10:24, 5 September 2018

OCA at 18:40, 1 August 2018

OCA: Protected "5zib" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 5zib is ON HOLD Authors: Description: Category: Unreleased Structures