5zjg - Revision history

OCA at 08:57, 22 November 2023

OCA — Wed, 22 Nov 2023 08:57:43 GMT

←Older revision		Revision as of 08:57, 22 November 2023
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	<StructureSection load='5zjg' size='340' side='right'caption='[[5zjg]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='5zjg' size='340' side='right'caption='[[5zjg]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zjg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"pseudomonas_azelaica"_janota-bassalik_et_al._1971 "pseudomonas azelaica" janota-bassalik et al. 1971~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZJG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zjg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_nitroreducens Pseudomonas nitroreducens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZJG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZJG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GLY:GLYCINE'>GLY</scene>~~, ~~<scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.702Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SAMN05216209_3923 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=46680 "Pseudomonas azelaica" Janota-Bassalik et al. 1971])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zjg OCA], [~~http~~://pdbe.org/5zjg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zjg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zjg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zjg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zjg OCA], [https://pdbe.org/5zjg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zjg RCSB], [https://www.ebi.ac.uk/pdbsum/5zjg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zjg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Pseudomonas azelaica janota-bassalik et al. 1971]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hibi, T~~]]	+	[[Category: Pseudomonas nitroreducens]]
-	[[Category: ~~Imaoka, M]]~~	+	[[Category: Hibi T]]
-	~~[[Category: Itoh,~~ T]]	+	[[Category: Imaoka M]]
-	[[Category: ~~Wakayama,~~ M]]	+	[[Category: Itoh T]]
-	[[Category: ~~Enzyme~~]]	+	[[Category: Wakayama M]]
-	[[Category: ~~Protein engineering]]~~	+
-	~~[[Category: Reaction specificity]]~~	+
-	~~[[Category: Substrate specificity]]~~	+
-	~~[[Category: Theanine synthesis]]~~	+
-	~~[[Category: Transferase]]~~	+
-	~~[[Category: Transpeptidation~~]]	+

OCA at 21:20, 28 October 2020

OCA — Wed, 28 Oct 2020 21:20:44 GMT

←Older revision		Revision as of 21:20, 28 October 2020
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	==Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly==		==Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly==
-	<StructureSection load='5zjg' size='340' side='right' caption='[[5zjg]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='5zjg' size='340' side='right'caption='[[5zjg]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zjg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZJG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca.weizmann.ac~~.~~il/oca-docs~~/fgij/fg.htm?mol=5ZJG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zjg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"pseudomonas_azelaica"_janota-bassalik_et_al._1971 "pseudomonas azelaica" janota-bassalik et al. 1971]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZJG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZJG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zjg OCA], [http://pdbe.org/5zjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zjg RCSB], [http://www.ebi.ac.uk/pdbsum/5zjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zjg ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SAMN05216209_3923 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=46680 "Pseudomonas azelaica" Janota-Bassalik et al. 1971])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zjg OCA], [http://pdbe.org/5zjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zjg RCSB], [http://www.ebi.ac.uk/pdbsum/5zjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zjg ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Pseudomonas azelaica janota-bassalik et al. 1971]]
		+	[[Category: Large Structures]]
	[[Category: Hibi, T]]		[[Category: Hibi, T]]
	[[Category: Imaoka, M]]		[[Category: Imaoka, M]]

OCA at 11:58, 16 January 2019

OCA — Wed, 16 Jan 2019 11:58:57 GMT

←Older revision		Revision as of 11:58, 16 January 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zjg is ~~ON HOLD until Paper~~ Publication	+	==Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly==
		+	<StructureSection load='5zjg' size='340' side='right' caption='[[5zjg]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zjg]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZJG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZJG FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zjg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zjg OCA], [http://pdbe.org/5zjg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zjg RCSB], [http://www.ebi.ac.uk/pdbsum/5zjg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zjg ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Theanine (gamma-glutamylethylamide) is an amino acid analog that reduces blood pressure and improves immune responses. The Upsilon-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens IFO12694 (PnGGT) has a unique preference for primary amines as Upsilon-glutamyl acceptors over standard L-amino acids and peptides. This characteristic is useful for the synthesis of theanine. We used X-ray crystallographic analysis to understand the structural basis of PnGGT's hydrolysis and transpeptidation reactions and to characterize its previously unidentified acceptor site. Structural studies of PnGGT have shown that key interactions between three residues (Trp385, Phe417, and Trp525) distinguish PnGGT from other GGTs. We studied the roles of these residues in the distinct biochemical properties of PnGGT using site-directed mutagenesis. All mutants showed a significant decrease in hydrolysis activity and an increase in transpeptidase activity, suggesting that the aromatic side chains of Trp385, Phe417, and Trp525 were involved in the recognition of acceptor substrates. Abbreviations: Upsilon-glutamyl peptide, theanine, X-ray crystallography.

-	~~Authors: Hibi~~, T., Imaoka, M., ~~Itoh~~, T., Wakayama, M.	+	Crystal structure analysis and enzymatic characterization of gamma-glutamyltranspeptidase from Pseudomonas nitroreducens.,Hibi T, Imaoka M, Shimizu Y, Itoh T, Wakayama M Biosci Biotechnol Biochem. 2018 Dec 3:1-8. doi: 10.1080/09168451.2018.1547104. PMID:30507352<ref>PMID:30507352</ref>

-	~~Description: Gamma~~-~~glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly~~-~~Gly~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Itoh, T]]~~	+	<div class="pdbe-citations 5zjg" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
	[[Category: Hibi, T]]		[[Category: Hibi, T]]
	[[Category: Imaoka, M]]		[[Category: Imaoka, M]]
		+	[[Category: Itoh, T]]
	[[Category: Wakayama, M]]		[[Category: Wakayama, M]]
		+	[[Category: Enzyme]]
		+	[[Category: Protein engineering]]
		+	[[Category: Reaction specificity]]
		+	[[Category: Substrate specificity]]
		+	[[Category: Theanine synthesis]]
		+	[[Category: Transferase]]
		+	[[Category: Transpeptidation]]

OCA at 03:48, 25 April 2018

OCA — Wed, 25 Apr 2018 03:48:58 GMT

←Older revision		Revision as of 03:48, 25 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zjg is ON HOLD	+	The entry 5zjg is ON HOLD until Paper Publication

	Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M.		Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M.

OCA at 12:18, 11 April 2018

OCA — Wed, 11 Apr 2018 12:18:21 GMT

←Older revision		Revision as of 12:18, 11 April 2018
Line 5:		Line 5:
	Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M.		Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M.

-	Description: ~~gamma~~-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly	+	Description: Gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Itoh, T]]		[[Category: Itoh, T]]

OCA: Protected "5zjg" [edit=sysop:move=sysop]

OCA — Wed, 28 Mar 2018 05:11:37 GMT

Protected "5zjg" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:11, 28 March 2018

OCA: New page: '''Unreleased structure''' The entry 5zjg is ON HOLD Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M. Description: gamma-glutamyltranspeptidase from Pseudomonas nitroreducens comp...

OCA — Wed, 28 Mar 2018 05:11:36 GMT

New page: '''Unreleased structure''' The entry 5zjg is ON HOLD Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M. Description: gamma-glutamyltranspeptidase from Pseudomonas nitroreducens comp...

New page

'''Unreleased structure'''

The entry 5zjg is ON HOLD

Authors: Hibi, T., Imaoka, M., Itoh, T., Wakayama, M.

Description: gamma-glutamyltranspeptidase from Pseudomonas nitroreducens complexed with Gly-Gly
[[Category: Unreleased Structures]]
[[Category: Itoh, T]]
[[Category: Hibi, T]]
[[Category: Imaoka, M]]
[[Category: Wakayama, M]]