5zlh - Revision history

OCA at 10:28, 27 March 2024

OCA — Wed, 27 Mar 2024 10:28:17 GMT

←Older revision		Revision as of 10:28, 27 March 2024
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	<StructureSection load='5zlh' size='340' side='right'caption='[[5zlh]], [[Resolution\|resolution]] 3.40Å' scene=''>		<StructureSection load='5zlh' size='340' side='right'caption='[[5zlh]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zlh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZLH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zlh]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZLH FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MNH:MANGANESE+PROTOPORPHYRIN+IX'>MNH</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5zis\|5zis]]~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MNH:MANGANESE+PROTOPORPHYRIN+IX'>MNH</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlh OCA], [~~http~~://pdbe.org/5zlh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zlh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zlh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlh OCA], [https://pdbe.org/5zlh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zlh RCSB], [https://www.ebi.ac.uk/pdbsum/5zlh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlh ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/CPXB_PRIM2 CPXB_PRIM2] Functions as a fatty acid monooxygenase (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Catalyzes hydroxylation of fatty acids at omega-1, omega-2 and omega-3 positions (PubMed:1727637, PubMed:21875028). Shows activity toward medium and long-chain fatty acids, with optimum chain lengths of 12, 14 and 16 carbons (lauric, myristic, and palmitic acids). Able to metabolize some of these primary metabolites to secondary and tertiary products (PubMed:1727637). Marginal activity towards short chain lengths of 8-10 carbons (PubMed:1727637, PubMed:18619466). Hydroxylates highly branched fatty acids, which play an essential role in membrane fluidity regulation (PubMed:16566047). Also displays a NADPH-dependent reductase activity in the C-terminal domain, which allows electron transfer from NADPH to the heme iron of the cytochrome P450 N-terminal domain (PubMed:3106359, PubMed:1727637, PubMed:16566047, PubMed:7578081, PubMed:11695892, PubMed:14653735, PubMed:16403573, PubMed:18004886, PubMed:17077084, PubMed:17868686, PubMed:18298086, PubMed:18619466, PubMed:18721129, PubMed:19492389, PubMed:20180779, PubMed:21110374, PubMed:21875028). Involved in inactivation of quorum sensing signals of other competing bacteria by oxidazing efficiently acyl homoserine lactones (AHLs), molecules involved in quorum sensing signaling pathways, and their lactonolysis products acyl homoserines (AHs) (PubMed:18020460).<ref>PMID:11695892</ref> <ref>PMID:14653735</ref> <ref>PMID:16403573</ref> <ref>PMID:16566047</ref> <ref>PMID:17077084</ref> <ref>PMID:1727637</ref> <ref>PMID:17868686</ref> <ref>PMID:18004886</ref> <ref>PMID:18020460</ref> <ref>PMID:18298086</ref> <ref>PMID:18619466</ref> <ref>PMID:18721129</ref> <ref>PMID:19492389</ref> <ref>PMID:20180779</ref> <ref>PMID:21110374</ref> <ref>PMID:21875028</ref> <ref>PMID:3106359</ref> <ref>PMID:7578081</ref>
-	~~Haem substitution is an effective approach to tweak the function of haemoproteins~~. ~~Herein, we report~~ a ~~facile haem substitution method for self-sufficient cytochrome P450BM3~~ (~~CYP102A1~~) ~~from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus~~. ~~We successfully constructed Mn~~-~~substituted BM3~~ and ~~investigated its catalytic~~ activity.	+
-		+
-	~~Reconstitution~~ of ~~full~~-~~length P450BM3 with~~ an ~~artificial metal complex by utilising the transpeptidase Sortase A~~.,~~Omura K~~, ~~Aiba Y~~, ~~Onoda H~~, ~~Stanfield JK~~, ~~Ariyasu S~~, ~~Sugimoto H~~, ~~Shiro Y~~, ~~Shoji O~~, ~~Watanabe Y Chem Commun (Camb~~). ~~2018 Jul 12;54~~(57):~~7892-7895~~. ~~doi~~: ~~10.1039~~/~~c8cc02760a.~~ PMID:~~29845154~~<ref>PMID:~~29845154~~</ref>	+
-		+
-	~~From MEDLINE®~~/~~PubMed®, a database of the U.S. National Library of Medicine.~~<br>	+
-	</~~div~~>	+
-	<~~div class="pdbe-citations 5zlh" style="background-color~~:~~#fffaf0;"~~></~~div~~>	+

	==See Also==		==See Also==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aiba, Y]]	+	[[Category: Priestia megaterium]]
-	[[Category: Omura, K]]	+	[[Category: Aiba Y]]
-	[[Category: Onoda, H]]	+	[[Category: Omura K]]
-	[[Category: Shoji, O]]	+	[[Category: Onoda H]]
-	[[Category: Sugimoto, H]]	+	[[Category: Shoji O]]
-	[[Category: Watanabe, Y]]	+	[[Category: Sugimoto H]]
-	~~[[Category: Cytochrome p450]]~~	+	[[Category: Watanabe Y]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 21:21, 28 October 2020

OCA — Wed, 28 Oct 2020 21:21:29 GMT

←Older revision		Revision as of 21:21, 28 October 2020
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	==Crystal structure of Mn-ProtoporphyrinIX-reconstituted P450BM3==		==Crystal structure of Mn-ProtoporphyrinIX-reconstituted P450BM3==
-	<StructureSection load='5zlh' size='340' side='right' caption='[[5zlh]], [[Resolution\|resolution]] 3.40Å' scene=''>	+	<StructureSection load='5zlh' size='340' side='right'caption='[[5zlh]], [[Resolution\|resolution]] 3.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zlh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZLH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zlh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZLH FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MNH:MANGANESE+PROTOPORPHYRIN+IX'>MNH</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MNH:MANGANESE+PROTOPORPHYRIN+IX'>MNH</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zis\|5zis]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zis\|5zis]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlh OCA], [http://pdbe.org/5zlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlh RCSB], [http://www.ebi.ac.uk/pdbsum/5zlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlh OCA], [http://pdbe.org/5zlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlh RCSB], [http://www.ebi.ac.uk/pdbsum/5zlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</div>		</div>
	<div class="pdbe-citations 5zlh" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5zlh" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Cytochrome P450 3D structures\|Cytochrome P450 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Aiba, Y]]		[[Category: Aiba, Y]]
	[[Category: Omura, K]]		[[Category: Omura, K]]

OCA at 16:09, 15 August 2018

OCA — Wed, 15 Aug 2018 16:09:28 GMT

←Older revision		Revision as of 16:09, 15 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zlh is ~~ON HOLD~~	+	==Crystal structure of Mn-ProtoporphyrinIX-reconstituted P450BM3==
		+	<StructureSection load='5zlh' size='340' side='right' caption='[[5zlh]], [[Resolution\|resolution]] 3.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zlh]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZLH FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MNH:MANGANESE+PROTOPORPHYRIN+IX'>MNH</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zis\|5zis]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zlh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlh OCA], [http://pdbe.org/5zlh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlh RCSB], [http://www.ebi.ac.uk/pdbsum/5zlh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlh ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

-	~~Authors: Omura~~, K., Aiba, Y., Onoda, H., ~~Sugimoto~~, H., ~~Shoji~~, O., Watanabe, Y.	+	Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A.,Omura K, Aiba Y, Onoda H, Stanfield JK, Ariyasu S, Sugimoto H, Shiro Y, Shoji O, Watanabe Y Chem Commun (Camb). 2018 Jul 12;54(57):7892-7895. doi: 10.1039/c8cc02760a. PMID:29845154<ref>PMID:29845154</ref>

-	~~Description: Crystal structure~~ of Mn-~~ProtoporphyrinIX~~-~~reconstituted P450BM3~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5zlh" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Aiba, Y]]
		+	[[Category: Omura, K]]
	[[Category: Onoda, H]]		[[Category: Onoda, H]]
	[[Category: Shoji, O]]		[[Category: Shoji, O]]
	[[Category: Sugimoto, H]]		[[Category: Sugimoto, H]]
-	[[Category: Aiba, Y]]
-	[[Category: Omura, K]]
	[[Category: Watanabe, Y]]		[[Category: Watanabe, Y]]
		+	[[Category: Cytochrome p450]]
		+	[[Category: Oxidoreductase]]

OCA at 19:39, 9 August 2018

OCA — Thu, 09 Aug 2018 19:39:27 GMT

←Older revision		Revision as of 19:39, 9 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zlh is ON HOLD ~~until Paper Publication~~	+	The entry 5zlh is ON HOLD

	Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y.		Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y.

OCA at 04:43, 9 May 2018

OCA — Wed, 09 May 2018 04:43:08 GMT

←Older revision		Revision as of 04:43, 9 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zlh is ON HOLD	+	The entry 5zlh is ON HOLD until Paper Publication

	Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y.		Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Onoda, H]]		[[Category: Onoda, H]]
-	[[Category: Sugimoto, H]]
-	[[Category: Omura, K]]
	[[Category: Shoji, O]]		[[Category: Shoji, O]]
		+	[[Category: Sugimoto, H]]
	[[Category: Aiba, Y]]		[[Category: Aiba, Y]]
		+	[[Category: Omura, K]]
	[[Category: Watanabe, Y]]		[[Category: Watanabe, Y]]

OCA: Protected "5zlh" [edit=sysop:move=sysop]

OCA — Wed, 04 Apr 2018 04:34:29 GMT

Protected "5zlh" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 4 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zlh is ON HOLD Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y. Description: Crystal structure of Mn-ProtoporphyrinI...

OCA — Wed, 04 Apr 2018 04:34:28 GMT

New page: '''Unreleased structure''' The entry 5zlh is ON HOLD Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y. Description: Crystal structure of Mn-ProtoporphyrinI...

New page

'''Unreleased structure'''

The entry 5zlh is ON HOLD

Authors: Omura, K., Aiba, Y., Onoda, H., Sugimoto, H., Shoji, O., Watanabe, Y.

Description: Crystal structure of Mn-ProtoporphyrinIX-reconstituted P450BM3
[[Category: Unreleased Structures]]
[[Category: Onoda, H]]
[[Category: Sugimoto, H]]
[[Category: Omura, K]]
[[Category: Shoji, O]]
[[Category: Aiba, Y]]
[[Category: Watanabe, Y]]