5zli - Revision history

OCA at 10:28, 27 March 2024

OCA — Wed, 27 Mar 2024 10:28:20 GMT

←Older revision		Revision as of 10:28, 27 March 2024
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	<StructureSection load='5zli' size='340' side='right'caption='[[5zli]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='5zli' size='340' side='right'caption='[[5zli]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zli]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=5ZLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zli]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_26695 Helicobacter pylori 26695]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZLI FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>~~[http://en.wikipedia.org/wiki/Glutamate~~-~~-ammonia_ligase Glutamate--ammonia ligase]~~, ~~with EC number~~ [~~http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.~~2 6.~~3.1.2] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=5zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zli OCA], [~~http~~://pdbe.org/5zli PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zli RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zli PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zli ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zli OCA], [https://pdbe.org/5zli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zli RCSB], [https://www.ebi.ac.uk/pdbsum/5zli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zli ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GLN1B_HELPY GLN1B_HELPY]] Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.[UniProtKB:P0A1P6]	+	[https://www.uniprot.org/uniprot/GLN1B_HELPY GLN1B_HELPY] Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.[UniProtKB:P0A1P6]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Glutamine synthetase (GS) is an enzyme that regulates nitrogen metabolism and synthesizes glutamine via glutamate, ATP, and ammonia. GS is a homo-oligomeric protein of eight, ten, or twelve subunits, and each subunit-subunit interface has its own active site. GS can be divided into GS I, GS II, and GS III. GS I and GS III form dodecamer in bacteria and archaea, whereas GS II form decamer in eukaryotes. GS I can be further subdivided into GS I-alpha and GS I-beta according to its sequence and regulatory mechanism. GS is an essential protein for the survival of Helicobacter pylori which its infection could promote gastroduodenal diseases. Here, we determined the crystal structures of the GS from H. pylori (Hpy GS) in its apo- and substrate-bound forms at 2.8 A and 2.9 A resolution, respectively. Hpy GS formed a dodecamer composed of two hexameric rings stacked face-to-face. Hpy GS, which belongs to GS I, cannot be clearly classified as either GS I-alpha or GS I-beta based on its sequence and regulatory mechanism. In this study, we propose that Hpy GS could be classified as a new GS-I subfamily and provide structural information on the apo- and substrate-bound forms of the protein.	+
-		+
-	Structural Analysis of Glutamine Synthetase from Helicobacter pylori.,Joo HK, Park YW, Jang YY, Lee JY Sci Rep. 2018 Aug 3;8(1):11657. doi: 10.1038/s41598-018-30191-5. PMID:30076387<ref>PMID:30076387</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5zli" style="background-color:#fffaf0;"></div>~~	+

	==See Also==		==See Also==
	*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]		*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Glutamate--ammonia ligase~~]]	+	[[Category: Helicobacter pylori 26695]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Joo~~, H K~~]]	+	[[Category: Joo HK]]
-	[[Category: Lee~~, J Y]]~~	+	[[Category: Lee JY]]
-	~~[[Category: Enzyme]]~~	+
-	~~[[Category: Glutamine synthetase]]~~	+
-	~~[[Category: Helicobacter pylori]]~~	+
-	~~[[Category: Ligase]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 21:21, 28 October 2020

OCA — Wed, 28 Oct 2020 21:21:30 GMT

←Older revision		Revision as of 21:21, 28 October 2020
Line 1:		Line 1:

	==Crystal structure of glutamine synthetase from helicobacter pylori==		==Crystal structure of glutamine synthetase from helicobacter pylori==
-	<StructureSection load='5zli' size='340' side='right' caption='[[5zli]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='5zli' size='340' side='right'caption='[[5zli]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zli]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLI OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZLI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zli]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5ZLI FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zli OCA], [http://pdbe.org/5zli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zli RCSB], [http://www.ebi.ac.uk/pdbsum/5zli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zli ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zli OCA], [http://pdbe.org/5zli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zli RCSB], [http://www.ebi.ac.uk/pdbsum/5zli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zli ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 5zli" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5zli" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glutamine synthetase 3D structures\|Glutamine synthetase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 23:		Line 26:
	</StructureSection>		</StructureSection>
	[[Category: Glutamate--ammonia ligase]]		[[Category: Glutamate--ammonia ligase]]
		+	[[Category: Large Structures]]
	[[Category: Joo, H K]]		[[Category: Joo, H K]]
	[[Category: Lee, J Y]]		[[Category: Lee, J Y]]

OCA at 07:21, 29 August 2018

OCA — Wed, 29 Aug 2018 07:21:46 GMT

←Older revision		Revision as of 07:21, 29 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zli is ~~ON HOLD~~	+	==Crystal structure of glutamine synthetase from helicobacter pylori==
		+	<StructureSection load='5zli' size='340' side='right' caption='[[5zli]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zli]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZLI FirstGlance]. <br>
		+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate--ammonia_ligase Glutamate--ammonia ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.1.2 6.3.1.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zli OCA], [http://pdbe.org/5zli PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zli RCSB], [http://www.ebi.ac.uk/pdbsum/5zli PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zli ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLN1B_HELPY GLN1B_HELPY]] Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.[UniProtKB:P0A1P6]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Glutamine synthetase (GS) is an enzyme that regulates nitrogen metabolism and synthesizes glutamine via glutamate, ATP, and ammonia. GS is a homo-oligomeric protein of eight, ten, or twelve subunits, and each subunit-subunit interface has its own active site. GS can be divided into GS I, GS II, and GS III. GS I and GS III form dodecamer in bacteria and archaea, whereas GS II form decamer in eukaryotes. GS I can be further subdivided into GS I-alpha and GS I-beta according to its sequence and regulatory mechanism. GS is an essential protein for the survival of Helicobacter pylori which its infection could promote gastroduodenal diseases. Here, we determined the crystal structures of the GS from H. pylori (Hpy GS) in its apo- and substrate-bound forms at 2.8 A and 2.9 A resolution, respectively. Hpy GS formed a dodecamer composed of two hexameric rings stacked face-to-face. Hpy GS, which belongs to GS I, cannot be clearly classified as either GS I-alpha or GS I-beta based on its sequence and regulatory mechanism. In this study, we propose that Hpy GS could be classified as a new GS-I subfamily and provide structural information on the apo- and substrate-bound forms of the protein.

-	~~Authors:~~ Joo, ~~H.K.~~, ~~Lee~~, J.Y.	+	Structural Analysis of Glutamine Synthetase from Helicobacter pylori.,Joo HK, Park YW, Jang YY, Lee JY Sci Rep. 2018 Aug 3;8(1):11657. doi: 10.1038/s41598-018-30191-5. PMID:30076387<ref>PMID:30076387</ref>

-	~~Description: Crystal structure~~ of ~~glutamine synthetase from helicobacter pylori~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: Lee, J.Y]]	+	<div class="pdbe-citations 5zli" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Joo, H.K~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Glutamate--ammonia ligase]]
		+	[[Category: Joo, H K]]
		+	[[Category: Lee, J Y]]
		+	[[Category: Enzyme]]
		+	[[Category: Glutamine synthetase]]
		+	[[Category: Helicobacter pylori]]
		+	[[Category: Ligase]]
		+	[[Category: Structural protein]]

OCA at 12:19, 11 April 2018

OCA — Wed, 11 Apr 2018 12:19:17 GMT

←Older revision		Revision as of 12:19, 11 April 2018
Line 5:		Line 5:
	Authors: Joo, H.K., Lee, J.Y.		Authors: Joo, H.K., Lee, J.Y.

-	Description: ~~crystal~~ structure of glutamine synthetase from helicobacter pylori	+	Description: Crystal structure of glutamine synthetase from helicobacter pylori
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Lee, J.Y]]		[[Category: Lee, J.Y]]
	[[Category: Joo, H.K]]		[[Category: Joo, H.K]]

OCA: Protected "5zli" [edit=sysop:move=sysop]

OCA — Wed, 04 Apr 2018 04:34:32 GMT

Protected "5zli" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 4 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zli is ON HOLD Authors: Joo, H.K., Lee, J.Y. Description: crystal structure of glutamine synthetase from helicobacter pylori [[Category: Unrelease...

OCA — Wed, 04 Apr 2018 04:34:31 GMT

New page: '''Unreleased structure''' The entry 5zli is ON HOLD Authors: Joo, H.K., Lee, J.Y. Description: crystal structure of glutamine synthetase from helicobacter pylori [[Category: Unrelease...

New page

'''Unreleased structure'''

The entry 5zli is ON HOLD

Authors: Joo, H.K., Lee, J.Y.

Description: crystal structure of glutamine synthetase from helicobacter pylori
[[Category: Unreleased Structures]]
[[Category: Lee, J.Y]]
[[Category: Joo, H.K]]