5zlk - Revision history

OCA at 08:59, 22 November 2023

OCA — Wed, 22 Nov 2023 08:59:52 GMT

←Older revision		Revision as of 08:59, 22 November 2023
Line 3:		Line 3:
	<StructureSection load='5zlk' size='340' side='right'caption='[[5zlk]], [[Resolution\|resolution]] 2.60Å' scene=''>		<StructureSection load='5zlk' size='340' side='right'caption='[[5zlk]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zlk]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bacsu Bacsu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZLK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zlk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZLK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CU:COPPER+(II)+ION'>CU</scene~~>, ~~<scene name='pdbligand=EDO:1,~~2~~-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[5zlj\|5zlj]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<~~tr id~~='~~gene~~'><~~td class="sblockLbl"~~><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">cotA, pig, BSU06300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlk OCA], [https://pdbe.org/5zlk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zlk RCSB], [https://www.ebi.ac.uk/pdbsum/5zlk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlk ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlk OCA], [~~http~~://pdbe.org/5zlk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zlk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zlk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlk ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.	+	[https://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU] Involved in brown pigmentation during sporogenesis.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 5zlk" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 5zlk" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Laccase 3D structures\|Laccase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacsu~~]]	+	[[Category: Bacillus subtilis subsp. subtilis str. 168]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Liu~~, Z C~~]]	+	[[Category: Liu ZC]]
-	[[Category: Wang~~, G G~~]]	+	[[Category: Wang GG]]
-	[[Category: Xie, T]]	+	[[Category: Xie T]]
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 16:04, 29 January 2020

OCA — Wed, 29 Jan 2020 16:04:09 GMT

←Older revision		Revision as of 16:04, 29 January 2020
Line 1:		Line 1:

	==Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0==		==Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0==
-	<StructureSection load='5zlk' size='340' side='right' caption='[[5zlk]], [[Resolution\|resolution]] 2.60Å' scene=''>	+	<StructureSection load='5zlk' size='340' side='right'caption='[[5zlk]], [[Resolution\|resolution]] 2.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zlk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZLK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zlk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZLK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zlj\|5zlj]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zlj\|5zlj]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cotA, pig, BSU06300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlk OCA], [http://pdbe.org/5zlk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlk RCSB], [http://www.ebi.ac.uk/pdbsum/5zlk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlk OCA], [http://pdbe.org/5zlk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlk RCSB], [http://www.ebi.ac.uk/pdbsum/5zlk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlk ProSAT]</span></td></tr>
	</table>		</table>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacsu]]
		+	[[Category: Large Structures]]
	[[Category: Liu, Z C]]		[[Category: Liu, Z C]]
	[[Category: Wang, G G]]		[[Category: Wang, G G]]
	[[Category: Xie, T]]		[[Category: Xie, T]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 05:27, 16 May 2018

OCA — Wed, 16 May 2018 05:27:41 GMT

←Older revision		Revision as of 05:27, 16 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zlk is ~~ON HOLD until Mar 28 2020~~	+	==Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0==
		+	<StructureSection load='5zlk' size='340' side='right' caption='[[5zlk]], [[Resolution\|resolution]] 2.60Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zlk]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZLK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZLK FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5zlj\|5zlj]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zlk OCA], [http://pdbe.org/5zlk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zlk RCSB], [http://www.ebi.ac.uk/pdbsum/5zlk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zlk ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/COTA_BACSU COTA_BACSU]] Involved in brown pigmentation during sporogenesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In laccase, type-1 copper (Cu1) was connected to the trinuclear copper center (TNC) by the conserved Cys-His bridge. An allosteric coupling between the two redox sites has been reported; however, the molecular mechanism underlining the allosteric coupling is unknown. In this study, ligands of the two type 3 copper sites, including His491 and His493, in CotA were mutated to Cys or Ala. The crystal structures revealed that mutation at His491 and His493 caused rearrangement of the hydrogen bond network and geometric distortion of the TNC, which severely impaired the activities of mutants H493A, H493C and H491C. In addition, the change in TNC affected the hydrogen bonds around Cys492 in the mutants and led to the Cu1 was partially reduced. These results not only decipher the mechanism of allosteric coupling between Cu1 and TNC in laccase but also pave the curb for laccase protein engineering.

-	~~Authors: Xie~~, T., Liu, Z~~.C.~~, Wang, G.G.	+	Structural Insight into the Allosteric Coupling of Cu1 Site and Trinuclear Cu Cluster in CotA Laccase.,Xie T, Liu Z, Wang G Chembiochem. 2018 May 3. doi: 10.1002/cbic.201800236. PMID:29722464<ref>PMID:29722464</ref>

-	~~Description: Mutation in~~ the ~~trinuclear site~~ of ~~CotA~~-~~laccase~~: ~~H493A mutant~~, ~~PH 8.0~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5zlk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Liu, Z C]]
		+	[[Category: Wang, G G]]
	[[Category: Xie, T]]		[[Category: Xie, T]]
-	[[Category: ~~Wang, G.G]]~~	+	[[Category: Oxidoreductase]]
-	~~[[Category: Liu, Z.C~~]]	+

OCA at 03:49, 25 April 2018

OCA — Wed, 25 Apr 2018 03:49:22 GMT

←Older revision		Revision as of 03:49, 25 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zlk is ON HOLD	+	The entry 5zlk is ON HOLD until Mar 28 2020

	Authors: Xie, T., Liu, Z.C., Wang, G.G.		Authors: Xie, T., Liu, Z.C., Wang, G.G.

OCA: Protected "5zlk" [edit=sysop:move=sysop]

OCA — Wed, 04 Apr 2018 04:34:51 GMT

Protected "5zlk" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:34, 4 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zlk is ON HOLD Authors: Xie, T., Liu, Z.C., Wang, G.G. Description: Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0 [[Catego...

OCA — Wed, 04 Apr 2018 04:34:49 GMT

New page: '''Unreleased structure''' The entry 5zlk is ON HOLD Authors: Xie, T., Liu, Z.C., Wang, G.G. Description: Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0 [[Catego...

New page

'''Unreleased structure'''

The entry 5zlk is ON HOLD

Authors: Xie, T., Liu, Z.C., Wang, G.G.

Description: Mutation in the trinuclear site of CotA-laccase: H493A mutant, PH 8.0
[[Category: Unreleased Structures]]
[[Category: Xie, T]]
[[Category: Wang, G.G]]
[[Category: Liu, Z.C]]