5zme - Revision history

OCA at 08:07, 3 April 2019

OCA — Wed, 03 Apr 2019 08:07:00 GMT

←Older revision		Revision as of 08:07, 3 April 2019
Line 3:		Line 3:
	<StructureSection load='5zme' size='340' side='right'caption='[[5zme]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='5zme' size='340' side='right'caption='[[5zme]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zme]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZME FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zme]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlre Chlre]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZME FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ARSA1, AS1, CHLREDRAFT_132949 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3055 CHLRE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zme OCA], [http://pdbe.org/5zme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zme RCSB], [http://www.ebi.ac.uk/pdbsum/5zme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zme ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zme OCA], [http://pdbe.org/5zme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zme RCSB], [http://www.ebi.ac.uk/pdbsum/5zme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zme ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/ASNA1_CHLRE ASNA1_CHLRE]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the chloroplast. Required for the accumulation of TOC34, an essential component of the outer chloroplast membrane translocon (TOC) complex (PubMed:23167510, PubMed:28382961). Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to chloroplast, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis (PubMed:28382961).<ref>PMID:23167510</ref> <ref>PMID:28382961</ref>		[[http://www.uniprot.org/uniprot/ASNA1_CHLRE ASNA1_CHLRE]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the chloroplast. Required for the accumulation of TOC34, an essential component of the outer chloroplast membrane translocon (TOC) complex (PubMed:23167510, PubMed:28382961). Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to chloroplast, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis (PubMed:28382961).<ref>PMID:23167510</ref> <ref>PMID:28382961</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Tail-anchored (TA) membrane proteins destined for the posttranslational pathway are safely delivered to the endoplasmic reticulum (ER) membrane by a well-known targeting factor, TRC40/Get3, in mammals and yeast. In contrast, the underlying mechanism for translocation of TA proteins in plants remains obscure. How this unique eukaryotic membrane-trafficking system correctly distinguishes different subsets of TA proteins destined for various organelles, including mitochondria, chloroplasts and the ER, is a key long-standing question. Here, we present crystal structures of algae ArsA1 (the Get3 homolog) in a distinct open state of nucleotide-free and AMPPNP-bound. This ~80-kDa protein possesses a monomeric architecture, with two ATPase domains in a single polypeptide chain. It is capable of binding chloroplast (TOC34 and TOC159) and mitochondrial (TOM7) TA proteins based on features of its transmembrane domain as well as the regions right before and after the transmembrane domain. Several helices located above the TA-binding groove comprise the interlocking hook-like motif implicated in TA substrate recognition by mutational analyses. Our data provide insights into the molecular basis of the highly specific selectivity of algae ArsA1 interactions with the correct sets of TA substrates before membrane targeting in plant cells. This article is protected by copyright. All rights reserved.
		+
		+	Structural analysis of chloroplast tail-anchored membrane protein recognition by ArsA1.,Lin TW, Chen CC, Wu SM, Chang YC, Li YC, Su YW, Hsiao CD, Chang HY Plant J. 2019 Mar 20. doi: 10.1111/tpj.14316. PMID:30891827<ref>PMID:30891827</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5zme" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chlre]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chang, H Y]]		[[Category: Chang, H Y]]

OCA at 07:42, 20 March 2019

OCA — Wed, 20 Mar 2019 07:42:03 GMT

←Older revision		Revision as of 07:42, 20 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zme is ~~ON HOLD until Paper Publication~~	+	==Nucleotide-free form of C. reinhardtii ArsA1==
-		+	<StructureSection load='5zme' size='340' side='right'caption='[[5zme]], [[Resolution\|resolution]] 3.60Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5zme]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZME OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZME FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zme FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zme OCA], [http://pdbe.org/5zme PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zme RCSB], [http://www.ebi.ac.uk/pdbsum/5zme PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zme ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ASNA1_CHLRE ASNA1_CHLRE]] ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the chloroplast. Required for the accumulation of TOC34, an essential component of the outer chloroplast membrane translocon (TOC) complex (PubMed:23167510, PubMed:28382961). Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to chloroplast, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis (PubMed:28382961).<ref>PMID:23167510</ref> <ref>PMID:28382961</ref>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang, H Y]]
		+	[[Category: Hsiao, C D]]
		+	[[Category: Lin, T W]]
		+	[[Category: Atpase]]
		+	[[Category: Chloroplast]]
		+	[[Category: Hydrolase-transport protein complex]]
		+	[[Category: Transport]]

OCA at 03:49, 25 April 2018

OCA — Wed, 25 Apr 2018 03:49:44 GMT

←Older revision		Revision as of 03:49, 25 April 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zme is ON HOLD	+	The entry 5zme is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5zme" [edit=sysop:move=sysop]

OCA — Wed, 11 Apr 2018 12:21:08 GMT

Protected "5zme" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:21, 11 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zme is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 11 Apr 2018 12:21:07 GMT

New page: '''Unreleased structure''' The entry 5zme is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zme is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]