5zon - Revision history

OCA at 09:02, 22 November 2023

OCA — Wed, 22 Nov 2023 09:02:09 GMT

←Older revision		Revision as of 09:02, 22 November 2023
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	==Histidinol phosphate phosphatase from Mycobacterium tuberculosis==		==Histidinol phosphate phosphatase from Mycobacterium tuberculosis==
-	<StructureSection load='5zon' size='340' side='right' caption='[[5zon]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='5zon' size='340' side='right'caption='[[5zon]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zon]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Myctu Myctu~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZON OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZON FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zon]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZON OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZON FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=GOL:GLYCEROL'>GOL</scene~~>, ~~<scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~hisN, impC, Rv3137 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=83332 MYCTU])</td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zon OCA], [https://pdbe.org/5zon PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zon RCSB], [https://www.ebi.ac.uk/pdbsum/5zon PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zon ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zon OCA], [~~http~~://pdbe.org/5zon PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zon RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zon PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zon ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HISN_MYCTU HISN_MYCTU]] Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine.	+	[https://www.uniprot.org/uniprot/HISN_MYCTU HISN_MYCTU] Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 24:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Histidinol-phosphatase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Myctu~~]]	+	[[Category: Mycobacterium tuberculosis H37Rv]]
-	[[Category: Biswal~~, B K~~]]	+	[[Category: Biswal BK]]
-	[[Category: Jha, B]]	+	[[Category: Jha B]]
-	[[Category: Kumar, D]]	+	[[Category: Kumar D]]
-	~~[[Category: Histidine biosynthetic pathway]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Phosphatase~~]]	+

OCA at 20:13, 19 September 2018

OCA — Wed, 19 Sep 2018 20:13:46 GMT

←Older revision		Revision as of 20:13, 19 September 2018
Line 3:		Line 3:
	<StructureSection load='5zon' size='340' side='right' caption='[[5zon]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='5zon' size='340' side='right' caption='[[5zon]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zon]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZON FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zon]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZON FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">hisN, impC, Rv3137 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zon OCA], [http://pdbe.org/5zon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zon RCSB], [http://www.ebi.ac.uk/pdbsum/5zon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zon ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zon OCA], [http://pdbe.org/5zon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zon RCSB], [http://www.ebi.ac.uk/pdbsum/5zon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zon ProSAT]</span></td></tr>
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	</StructureSection>		</StructureSection>
	[[Category: Histidinol-phosphatase]]		[[Category: Histidinol-phosphatase]]
		+	[[Category: Myctu]]
	[[Category: Biswal, B K]]		[[Category: Biswal, B K]]
	[[Category: Jha, B]]		[[Category: Jha, B]]

OCA at 07:19, 23 May 2018

OCA — Wed, 23 May 2018 07:19:07 GMT

←Older revision		Revision as of 07:19, 23 May 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zon is ~~ON HOLD until Paper~~ Publication	+	==Histidinol phosphate phosphatase from Mycobacterium tuberculosis==
		+	<StructureSection load='5zon' size='340' side='right' caption='[[5zon]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zon]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZON OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZON FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidinol-phosphatase Histidinol-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.15 3.1.3.15] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zon FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zon OCA], [http://pdbe.org/5zon PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zon RCSB], [http://www.ebi.ac.uk/pdbsum/5zon PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zon ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HISN_MYCTU HISN_MYCTU]] Catalyzes the dephosphorylation of histidinol-phosphate to histidinol, the direct precursor of histidine.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The absence of a histidine biosynthesis pathway in humans, coupled with histidine essentiality for survival of the important human pathogen Mycobacterium tuberculosis (Mtb), underscores the importance of the bacterial enzymes of this pathway as major anti-tuberculosis drug targets. However, the identity of the mycobacterial enzyme that functions as the histidinol phosphate phosphatase (HolPase) of this pathway remains to be established. Here, we demonstrate that the enzyme encoded by the Rv3137 gene, belonging to the inositol monophosphatase (IMPase) family, functions as the Mtb HolPase and specifically dephosphorylates histidinol phosphate. The crystal structure of Rv3137 in apo form enabled us to dissect its distinct structural features. Furthermore, the holo-complex structure revealed that a unique cocatalytic multi-zinc-assisted mode of substrate binding and catalysis is the hallmark of Mtb HolPase. Interestingly, the enzyme-substrate complex structure unveiled that although monomers possess individual catalytic sites, they share a common product-exit channel at the dimer interface. Furthermore, target-based screening against HolPase identified several small-molecule inhibitors of this enzyme. Taken together, our study unravels the missing enzyme link in the Mtb histidine biosynthesis pathway, augments our current mechanistic understanding of histidine production in Mtb, and has helped identify potential inhibitors of this bacterial pathway.

-	~~Authors~~:	+	Identification and structural characterization of a histidinol phosphate phosphatase from Mycobacterium tuberculosis.,Jha B, Kumar D, Sharma A, Dwivedy A, Singh R, Biswal BK J Biol Chem. 2018 May 11. pii: RA118.002299. doi: 10.1074/jbc.RA118.002299. PMID:29752410<ref>PMID:29752410</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5zon" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Histidinol-phosphatase]]
		+	[[Category: Biswal, B K]]
		+	[[Category: Jha, B]]
		+	[[Category: Kumar, D]]
		+	[[Category: Histidine biosynthetic pathway]]
		+	[[Category: Hydrolase]]
		+	[[Category: Phosphatase]]

OCA at 06:32, 2 May 2018

OCA — Wed, 02 May 2018 06:32:47 GMT

←Older revision		Revision as of 06:32, 2 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zon is ON HOLD	+	The entry 5zon is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5zon" [edit=sysop:move=sysop]

OCA — Wed, 18 Apr 2018 04:08:37 GMT

Protected "5zon" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:08, 18 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zon is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 18 Apr 2018 04:08:36 GMT

New page: '''Unreleased structure''' The entry 5zon is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zon is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]