5zov - Revision history

OCA at 09:02, 22 November 2023

OCA — Wed, 22 Nov 2023 09:02:17 GMT

←Older revision		Revision as of 09:02, 22 November 2023
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	==Inward-facing conformation of L-ascorbate transporter UlaA==		==Inward-facing conformation of L-ascorbate transporter UlaA==
-	<StructureSection load='5zov' size='340' side='right' caption='[[5zov]], [[Resolution\|resolution]] 3.33Å' scene=''>	+	<StructureSection load='5zov' size='340' side='right'caption='[[5zov]], [[Resolution\|resolution]] 3.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zov]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZOV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zov]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pasteurella_multocida Pasteurella multocida]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZOV FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ASC:ASCORBIC+ACID'>ASC</scene~~>, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.333Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~AZI96_02610 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zov OCA], [~~http~~://pdbe.org/5zov PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zov RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zov PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zov ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zov OCA], [https://pdbe.org/5zov PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zov RCSB], [https://www.ebi.ac.uk/pdbsum/5zov PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zov ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9CMQ1_PASMU Q9CMQ1_PASMU]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium multocidum lehmann and neumann 1899~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wang, J W~~]]	+	[[Category: Pasteurella multocida]]
-	[[Category: ~~L-ascorbate pt]]~~	+	[[Category: Wang JW]]
-	~~[[Category: Transport protein~~]]	+

OCA at 20:14, 19 September 2018

OCA — Wed, 19 Sep 2018 20:14:12 GMT

←Older revision		Revision as of 20:14, 19 September 2018
Line 3:		Line 3:
	<StructureSection load='5zov' size='340' side='right' caption='[[5zov]], [[Resolution\|resolution]] 3.33Å' scene=''>		<StructureSection load='5zov' size='340' side='right' caption='[[5zov]], [[Resolution\|resolution]] 3.33Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zov]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZOV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zov]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_multocidum"_lehmann_and_neumann_1899 "bacterium multocidum" lehmann and neumann 1899]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZOV FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">AZI96_02610 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=747 "Bacterium multocidum" Lehmann and Neumann 1899])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zov OCA], [http://pdbe.org/5zov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zov RCSB], [http://www.ebi.ac.uk/pdbsum/5zov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zov ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zov OCA], [http://pdbe.org/5zov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zov RCSB], [http://www.ebi.ac.uk/pdbsum/5zov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zov ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Various bacteria can ferment vitamin C (l-ascorbate) under anaerobic conditions via the phosphoenolpyruvate-dependent phosphotransferase system (PTS). The PTS(asc) system is composed of two soluble energy-coupling proteins (EI and HPr) and an enzyme II complex (EIIA, EIIB, and EIIC) for the anaerobic uptake of ascorbate and its phosphorylation to l-ascorbate 6-phosphate in vivo. Crystal structures of the ascorbate-bound EIIC component from Escherichia coli are available in outward-open and occluded conformations, suggesting a possible elevator mechanism of membrane transport. Despite these advances, it remains unclear how EIIC actually transports the substrate across the membrane and interacts with EIIB, which transfers its phosphate group to the EIIC-embedding ascorbate. Here, we present the crystal structure of the EIIC(asc) component from Pasteurella multocida in the inward-facing conformation. By comparing three conformational states, we confirmed the original proposed model: the ascorbate translocation can be achieved by a rigid-body movement of the substrate-binding core domain relative to the V motif domain, which brings along the transmembrane helices TM2 and TM7 of the V motif domain to undergo a winding at the pivotal positions. Together with an in vivo transport assay, we completed the picture of the transport cycle of the ascorbate superfamily of membrane-spanning EIIC components of the PTS system.
		+
		+	Inward-facing conformation of l-ascorbate transporter suggests an elevator mechanism.,Luo P, Dai S, Zeng J, Duan J, Shi H, Wang J Cell Discov. 2018 Jul 17;4:35. doi: 10.1038/s41421-018-0037-y. eCollection 2018. PMID:30038796<ref>PMID:30038796</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 5zov" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacterium multocidum lehmann and neumann 1899]]
	[[Category: Wang, J W]]		[[Category: Wang, J W]]
	[[Category: L-ascorbate pt]]		[[Category: L-ascorbate pt]]
	[[Category: Transport protein]]		[[Category: Transport protein]]

OCA at 18:41, 1 August 2018

OCA — Wed, 01 Aug 2018 18:41:19 GMT

←Older revision		Revision as of 18:41, 1 August 2018
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-	'''Unreleased structure'''

-	~~The entry 5zov is ON HOLD until Paper Publication~~	+	==Inward-facing conformation of L-ascorbate transporter UlaA==
-		+	<StructureSection load='5zov' size='340' side='right' caption='[[5zov]], [[Resolution\|resolution]] 3.33Å' scene=''>
-	~~Authors: Wang, J.W.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[5zov]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZOV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZOV FirstGlance]. <br>
-	~~Description:~~ Inward-facing conformation of L-ascorbate transporter UlaA	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ASC:ASCORBIC+ACID'>ASC</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zov FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zov OCA], [http://pdbe.org/5zov PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zov RCSB], [http://www.ebi.ac.uk/pdbsum/5zov PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zov ProSAT]</span></td></tr>
-	[[Category: Wang, J.W]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Wang, J W]]
		+	[[Category: L-ascorbate pt]]
		+	[[Category: Transport protein]]

OCA at 06:33, 2 May 2018

OCA — Wed, 02 May 2018 06:33:03 GMT

←Older revision		Revision as of 06:33, 2 May 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zov is ON HOLD	+	The entry 5zov is ON HOLD until Paper Publication

	Authors: Wang, J.W.		Authors: Wang, J.W.

OCA: Protected "5zov" [edit=sysop:move=sysop]

OCA — Wed, 25 Apr 2018 03:52:08 GMT

Protected "5zov" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:52, 25 April 2018

OCA: New page: '''Unreleased structure''' The entry 5zov is ON HOLD Authors: Wang, J.W. Description: Inward-facing conformation of L-ascorbate transporter UlaA Category: Unreleased Structures [[C...

OCA — Wed, 25 Apr 2018 03:52:07 GMT

New page: '''Unreleased structure''' The entry 5zov is ON HOLD Authors: Wang, J.W. Description: Inward-facing conformation of L-ascorbate transporter UlaA Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 5zov is ON HOLD

Authors: Wang, J.W.

Description: Inward-facing conformation of L-ascorbate transporter UlaA
[[Category: Unreleased Structures]]
[[Category: Wang, J.W]]