5ztm - Revision history

OCA at 09:06, 22 November 2023

OCA — Wed, 22 Nov 2023 09:06:52 GMT

←Older revision		Revision as of 09:06, 22 November 2023
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	<StructureSection load='5ztm' size='340' side='right'caption='[[5ztm]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='5ztm' size='340' side='right'caption='[[5ztm]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ztm]] is a 3 chain structure with sequence from [~~http://en.wikipedia.org/wiki/Drome Drome] and [http~~://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZTM OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZTM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ztm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZTM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZTM FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.899Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">mle, nap, CG11680 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=7227 DROME])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ztm OCA], [https://pdbe.org/5ztm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ztm RCSB], [https://www.ebi.ac.uk/pdbsum/5ztm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ztm ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ztm OCA], [~~http~~://pdbe.org/5ztm PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5ztm RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5ztm PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5ztm ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MLE_DROME MLE_DROME]] Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.<ref>PMID:1653648</ref>	+	[https://www.uniprot.org/uniprot/MLE_DROME MLE_DROME] Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.<ref>PMID:1653648</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Drome]]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: RNA helicase]]~~	+	[[Category: Lv MQ]]
-	[[Category: Lv~~, M Q~~]]	+	[[Category: Tang YJ]]
-	[[Category: Tang~~, Y J]]~~	+
-	~~[[Category: Helicase]]~~	+
-	~~[[Category: Hydrolase-rna complex]]~~	+
-	~~[[Category: Lncrna~~]]	+

OCA at 07:44, 24 April 2019

OCA — Wed, 24 Apr 2019 07:44:43 GMT

←Older revision		Revision as of 07:44, 24 April 2019
Line 1:		Line 1:

	==Crystal structure of MLE dsRBDs in complex with roX2 (R2H1)==		==Crystal structure of MLE dsRBDs in complex with roX2 (R2H1)==
-	<StructureSection load='5ztm' size='340' side='right' caption='[[5ztm]], [[Resolution\|resolution]] 2.90Å' scene=''>	+	<StructureSection load='5ztm' size='340' side='right'caption='[[5ztm]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5ztm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZTM FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5ztm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZTM FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mle, nap, CG11680 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ztm OCA], [http://pdbe.org/5ztm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ztm RCSB], [http://www.ebi.ac.uk/pdbsum/5ztm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ztm ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ztm OCA], [http://pdbe.org/5ztm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ztm RCSB], [http://www.ebi.ac.uk/pdbsum/5ztm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ztm ProSAT]</span></td></tr>
Line 23:		Line 24:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Drome]]
	[[Category: Drosophila melanogaster]]		[[Category: Drosophila melanogaster]]
		+	[[Category: Large Structures]]
	[[Category: RNA helicase]]		[[Category: RNA helicase]]
	[[Category: Lv, M Q]]		[[Category: Lv, M Q]]

OCA at 07:23, 6 March 2019

OCA — Wed, 06 Mar 2019 07:23:46 GMT

←Older revision		Revision as of 07:23, 6 March 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5ztm is ~~ON HOLD~~ ~~until May 04 2020~~	+	==Crystal structure of MLE dsRBDs in complex with roX2 (R2H1)==
		+	<StructureSection load='5ztm' size='340' side='right' caption='[[5ztm]], [[Resolution\|resolution]] 2.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5ztm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZTM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZTM FirstGlance]. <br>
		+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA_helicase RNA helicase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.4.13 3.6.4.13] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ztm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ztm OCA], [http://pdbe.org/5ztm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ztm RCSB], [http://www.ebi.ac.uk/pdbsum/5ztm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ztm ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MLE_DROME MLE_DROME]] Required in males for dosage compensation of X chromosome linked genes. Mle, msl-1 and msl-3 are colocalized on X chromosome. Each of the msl proteins requires all the other msls for wild-type X-chromosome binding. Probably unwinds double-stranded DNA and RNA in a 3' to 5' direction.<ref>PMID:1653648</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In Drosophila, dosage compensation globally upregulates the expression of genes located on male single X-chromosome. Maleless (MLE) helicase plays an essential role to incorporate the roX lncRNA into the dosage compensation complex (MSL-DCC), and such function is essentially dependent on its dsRNA-binding domains (dsRBDs). Here, we report a 2.90A crystal structure of tandem dsRBDs of MLE in complex with a 55mer stem-loop of roX2 (R2H1). MLE dsRBDs bind to R2H1 cooperatively and interact with two successive minor grooves and a major groove of R2H1, respectively. The recognition of R2H1 by MLE dsRBDs involves both shape- and sequence-specificity. Moreover, dsRBD2 displays a stronger RNA affinity than dsRBD1, and mutations of key residues in either MLE dsRBD remarkably reduce their affinities for roX2 both in vitro and in vivo. In Drosophila, the structure-based mle mutations generated using the CRISPR/Cas9 system, are partially male-lethal and indicate the inter-regulation among the components of the MSL-DCC at multiple levels. Hence, our research provides structural insights into the interactions between MLE dsRBDs and R2H1 and facilitates a deeper understanding of the mechanism by which MLE tandem dsRBDs play an indispensable role in specific recognition of roX and the assembly of the MSL-DCC in Drosophila dosage compensation.

-	~~Authors~~:	+	Structural insights reveal the specific recognition of roX RNA by the dsRNA-binding domains of the RNA helicase MLE and its indispensable role in dosage compensation in Drosophila.,Lv M, Yao Y, Li F, Xu L, Yang L, Gong Q, Xu YZ, Shi Y, Fan YJ, Tang Y Nucleic Acids Res. 2019 Jan 15. pii: 5289492. doi: 10.1093/nar/gky1308. PMID:30649456<ref>PMID:30649456</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 5ztm" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Drosophila melanogaster]]
		+	[[Category: RNA helicase]]
		+	[[Category: Lv, M Q]]
		+	[[Category: Tang, Y J]]
		+	[[Category: Helicase]]
		+	[[Category: Hydrolase-rna complex]]
		+	[[Category: Lncrna]]

OCA at 05:49, 23 May 2018

OCA — Wed, 23 May 2018 05:49:53 GMT

←Older revision		Revision as of 05:49, 23 May 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5ztm is ON HOLD	+	The entry 5ztm is ON HOLD until May 04 2020

	Authors:		Authors:

OCA: Protected "5ztm" [edit=sysop:move=sysop]

OCA — Wed, 09 May 2018 04:47:11 GMT

Protected "5ztm" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 9 May 2018

OCA: New page: '''Unreleased structure''' The entry 5ztm is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 09 May 2018 04:47:10 GMT

New page: '''Unreleased structure''' The entry 5ztm is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5ztm is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]