5zyr - Revision history

OCA at 10:29, 27 March 2024

OCA — Wed, 27 Mar 2024 10:29:56 GMT

←Older revision		Revision as of 10:29, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='5zyr' size='340' side='right'caption='[[5zyr]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='5zyr' size='340' side='right'caption='[[5zyr]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zyr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZYR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zyr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZYR FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=ACT:ACETATE+ION'>ACT</scene~~>, ~~<scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2000132Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[http:~~/~~/en.wikipedia.org/wiki/Flavin_reductase_(NADH) Flavin reductase (NADH)]~~, ~~with EC number [http~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.36 1.5.1.36]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyr OCA], [~~http~~://pdbe.org/5zyr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zyr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zyr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyr ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyr OCA], [https://pdbe.org/5zyr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zyr RCSB], [https://www.ebi.ac.uk/pdbsum/5zyr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HPAHR_ACIBA HPAHR_ACIBA]] Reductase component of a two-component system that supplies reduced FMN (FMNH2) to the oxygenase component to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetate (3,4-DHPA). Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the oxygenase component C2.<ref>PMID:11683878</ref> <ref>PMID:16042421</ref> <ref>PMID:17595116</ref>	+	[https://www.uniprot.org/uniprot/HPAHR_ACIBA HPAHR_ACIBA] Reductase component of a two-component system that supplies reduced FMN (FMNH2) to the oxygenase component to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetate (3,4-DHPA). Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the oxygenase component C2.<ref>PMID:11683878</ref> <ref>PMID:16042421</ref> <ref>PMID:17595116</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	p-Hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii catalyzes the hydroxylation of p-hydroxyphenylacetate (HPA) at the ortho position to yield 3,4-dihydroxyphenylacetate (DHPA). HPAH from A. baumannii is a two-component flavoprotein consisting of a smaller reductase (C(1)) component and a larger oxygenase (C(2)) component. The C(1) component supplies a reduced flavin in its free form to the C(2) counterpart for hydroxylation. In addition, HPA can bind to C(1) and enhance the flavin-reduction rate without becoming hydroxylated. The recombinant C(1) component was purified and crystallized using the microbatch method at 295 K. X-ray diffraction data were collected to 2.3 A resolution using synchrotron radiation on the BL13B1 beamline at NSRRC, Taiwan. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 47.78, b = 59.92, c = 211.85 A, and contained two molecules of C(1) per asymmetric unit.	+
-		+
-	Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii.,Oonanant W, Sucharitakul J, Chaiyen P, Yuvaniyama J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):720-3. doi:, 10.1107/S1744309112016909. Epub 2012 May 24. PMID:22684080<ref>PMID:22684080</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 5zyr" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Large Structures]]
-	[[Category: Chaiyen, P]]
-	[[Category: Oonanant, W]]
-	[[Category: Phongsak, T]]
-	[[Category: Sucharitakul, J]]
-	[[Category: Yuvaniyama, J]]
	[[Category: Acinetobacter baumannii]]		[[Category: Acinetobacter baumannii]]
-	[[Category: ~~Flavoprotein~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Monooxygenase~~]]	+	[[Category: Chaiyen P]]
-	[[Category: ~~Reductase~~]]	+	[[Category: Oonanant W]]
		+	[[Category: Phongsak T]]
		+	[[Category: Sucharitakul J]]
		+	[[Category: Yuvaniyama J]]

OCA at 22:40, 5 June 2019

OCA — Wed, 05 Jun 2019 22:40:30 GMT

←Older revision		Revision as of 22:40, 5 June 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 5zyr is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Crystal structure of the reductase (C1) component of p-hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii==
		+	<StructureSection load='5zyr' size='340' side='right'caption='[[5zyr]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zyr]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZYR FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Flavin_reductase_(NADH) Flavin reductase (NADH)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.36 1.5.1.36] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zyr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyr OCA], [http://pdbe.org/5zyr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zyr RCSB], [http://www.ebi.ac.uk/pdbsum/5zyr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyr ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HPAHR_ACIBA HPAHR_ACIBA]] Reductase component of a two-component system that supplies reduced FMN (FMNH2) to the oxygenase component to catalyze the hydroxylation of 4-hydroxyphenylacetic acid, leading to the production of 3,4-dihydroxyphenylacetate (3,4-DHPA). Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the oxygenase component C2.<ref>PMID:11683878</ref> <ref>PMID:16042421</ref> <ref>PMID:17595116</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	p-Hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii catalyzes the hydroxylation of p-hydroxyphenylacetate (HPA) at the ortho position to yield 3,4-dihydroxyphenylacetate (DHPA). HPAH from A. baumannii is a two-component flavoprotein consisting of a smaller reductase (C(1)) component and a larger oxygenase (C(2)) component. The C(1) component supplies a reduced flavin in its free form to the C(2) counterpart for hydroxylation. In addition, HPA can bind to C(1) and enhance the flavin-reduction rate without becoming hydroxylated. The recombinant C(1) component was purified and crystallized using the microbatch method at 295 K. X-ray diffraction data were collected to 2.3 A resolution using synchrotron radiation on the BL13B1 beamline at NSRRC, Taiwan. The crystal belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 47.78, b = 59.92, c = 211.85 A, and contained two molecules of C(1) per asymmetric unit.

-	~~Authors: Oonanant~~, W~~., Phongsak, T.~~, Sucharitakul, J., Chaiyen, P., Yuvaniyama, J.	+	Crystallization and preliminary X-ray analysis of the reductase component of p-hydroxyphenylacetate 3-hydroxylase from Acinetobacter baumannii.,Oonanant W, Sucharitakul J, Chaiyen P, Yuvaniyama J Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jun 1;68(Pt 6):720-3. doi:, 10.1107/S1744309112016909. Epub 2012 May 24. PMID:22684080<ref>PMID:22684080</ref>

-	~~Description: Crystal structure~~ of the ~~reductase (C1) component~~ of p-~~hydroxyphenylacetate 3~~-~~hydroxylase (HPAH) from Acinetobacter baumannii~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category~~: ~~Unreleased Structures]]~~	+	</div>
-	~~[[Category: Phongsak, T]]~~	+	<div class="pdbe-citations 5zyr" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Yuvaniyama, J~~]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Chaiyen, P]]		[[Category: Chaiyen, P]]
-	[[Category: Sucharitakul, J]]
	[[Category: Oonanant, W]]		[[Category: Oonanant, W]]
		+	[[Category: Phongsak, T]]
		+	[[Category: Sucharitakul, J]]
		+	[[Category: Yuvaniyama, J]]
		+	[[Category: Acinetobacter baumannii]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Monooxygenase]]
		+	[[Category: Reductase]]

OCA at 03:52, 20 June 2018

OCA — Wed, 20 Jun 2018 03:52:03 GMT

←Older revision		Revision as of 03:52, 20 June 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zyr is ON HOLD	+	The entry 5zyr is ON HOLD until Paper Publication

	Authors: Oonanant, W., Phongsak, T., Sucharitakul, J., Chaiyen, P., Yuvaniyama, J.		Authors: Oonanant, W., Phongsak, T., Sucharitakul, J., Chaiyen, P., Yuvaniyama, J.

OCA: Protected "5zyr" [edit=sysop:move=sysop]

OCA — Wed, 06 Jun 2018 04:11:23 GMT

Protected "5zyr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 6 June 2018

OCA: New page: '''Unreleased structure''' The entry 5zyr is ON HOLD Authors: Oonanant, W., Phongsak, T., Sucharitakul, J., Chaiyen, P., Yuvaniyama, J. Description: Crystal structure of the reductase ...

OCA — Wed, 06 Jun 2018 04:11:22 GMT

New page: '''Unreleased structure''' The entry 5zyr is ON HOLD Authors: Oonanant, W., Phongsak, T., Sucharitakul, J., Chaiyen, P., Yuvaniyama, J. Description: Crystal structure of the reductase ...

New page

'''Unreleased structure'''

The entry 5zyr is ON HOLD

Authors: Oonanant, W., Phongsak, T., Sucharitakul, J., Chaiyen, P., Yuvaniyama, J.

Description: Crystal structure of the reductase (C1) component of p-hydroxyphenylacetate 3-hydroxylase (HPAH) from Acinetobacter baumannii
[[Category: Unreleased Structures]]
[[Category: Phongsak, T]]
[[Category: Yuvaniyama, J]]
[[Category: Chaiyen, P]]
[[Category: Sucharitakul, J]]
[[Category: Oonanant, W]]