5zyx - Revision history

OCA at 10:35, 14 June 2023

OCA — Wed, 14 Jun 2023 10:35:51 GMT

←Older revision		Revision as of 10:35, 14 June 2023
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	==Solution NMR structure of K30 peptide in 10 mM dioctanoyl phosphatidylglycerol (D8PG)==		==Solution NMR structure of K30 peptide in 10 mM dioctanoyl phosphatidylglycerol (D8PG)==
-	<StructureSection load='5zyx' size='340' side='right'caption='[[5zyx~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='5zyx' size='340' side='right'caption='[[5zyx]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[5zyx]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5ZYX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[5zyx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZYX FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=5zyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyx OCA], [~~http~~://pdbe.org/5zyx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=5zyx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/5zyx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyx ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5zyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyx OCA], [https://pdbe.org/5zyx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5zyx RCSB], [https://www.ebi.ac.uk/pdbsum/5zyx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyx ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/A16L1_HUMAN A16L1_HUMAN] Crohn disease. Disease susceptibility is associated with variations affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A16L1_HUMAN A16L1_HUMAN] Plays an essential role in autophagy: interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to LC3 (MAP1LC3A, MAP1LC3B or MAP1LC3C), to produce a membrane-bound activated form of LC3 named LC3-II.<ref>PMID:23376921</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhunia, A]]	+	[[Category: Bhunia A]]
-	[[Category: Calligari, P]]	+	[[Category: Calligari P]]
-	[[Category: Mohid, A]]	+	[[Category: Mohid A]]
-	[[Category: Stella, L]]	+	[[Category: Stella L]]
-	~~[[Category: Antimicrobial protein]]~~	+
-	~~[[Category: D8pg]]~~	+
-	~~[[Category: K30]]~~	+
-	~~[[Category: Structure from cyana 2 1~~]]	+

OCA at 06:23, 19 June 2019

OCA — Wed, 19 Jun 2019 06:23:44 GMT

←Older revision		Revision as of 06:23, 19 June 2019
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Bhunia, A]]		[[Category: Bhunia, A]]
		+	[[Category: Calligari, P]]
	[[Category: Mohid, A]]		[[Category: Mohid, A]]
	[[Category: Stella, L]]		[[Category: Stella, L]]

OCA at 05:24, 12 June 2019

OCA — Wed, 12 Jun 2019 05:24:42 GMT

←Older revision		Revision as of 05:24, 12 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 5zyx is ~~ON HOLD until Paper~~ Publication	+	==Solution NMR structure of K30 peptide in 10 mM dioctanoyl phosphatidylglycerol (D8PG)==
		+	<StructureSection load='5zyx' size='340' side='right'caption='[[5zyx]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[5zyx]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZYX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ZYX FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5zyx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5zyx OCA], [http://pdbe.org/5zyx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5zyx RCSB], [http://www.ebi.ac.uk/pdbsum/5zyx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5zyx ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A synthetic antimicrobial peptide library based on the human autophagy 16 polypeptide has been developed. Designed acetylated peptides bearing lipids of different chain lengths, resulted in peptides with enhanced potency compared to the parent Atg16. A 21-residue fragment of Atg16 conjugated to 4-methylhexanoic acid (K30) emerged as the most potent antibacterial, with negligible hemolysis. Several studies, including microscopy, dye leakage and ITC were conducted to gain insight into the antibacterial mechanism of action of the peptide. Visual inspection using both SEM and TEM revealed the membranolytic effect of the peptide on bacterial cells. The selectivity of the peptide against bacterial cell membranes was also proven using dye leakage assays. ITC analysis revealed the exothermic nature of the binding interaction of the peptide to D8PG micelles. Three dimensional solution NMR structure of K30 in complex with dioctanoyl phosphatidylglycerol (D8PG) micelles revealed that the peptide adopts a helix-loop-helix structure in the presence of anionic membrane lipids mimicking bacterial membranes. Intermolecular NOEs between the peptide and lipid deciphered the location of the peptide in the bound state, which was subsequently supported by the paramagnetic relaxation enhancement (PRE) NMR experiment. Collectively, these results describe the structure function relationship of the peptide in the bacterial membrane.

-	~~Authors~~:	+	Design, Synthesis, Antibacterial Potential and Structural Characterization of N-acylated Derivatives of Human Autophagy 16 Polypeptide.,Varnava KG, Mohid AS, Calligari P, Stella L, Reynisson J, Bhunia A, Sarojini V Bioconjug Chem. 2019 May 30. doi: 10.1021/acs.bioconjchem.9b00290. PMID:31145591<ref>PMID:31145591</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 5zyx" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bhunia, A]]
		+	[[Category: Mohid, A]]
		+	[[Category: Stella, L]]
		+	[[Category: Antimicrobial protein]]
		+	[[Category: D8pg]]
		+	[[Category: K30]]
		+	[[Category: Structure from cyana 2 1]]

OCA at 06:06, 10 October 2018

OCA — Wed, 10 Oct 2018 06:06:12 GMT

←Older revision		Revision as of 06:06, 10 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 5zyx is ON HOLD	+	The entry 5zyx is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "5zyx" [edit=sysop:move=sysop]

OCA — Wed, 06 Jun 2018 04:11:50 GMT

Protected "5zyx" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 6 June 2018

OCA: New page: '''Unreleased structure''' The entry 5zyx is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 Jun 2018 04:11:48 GMT

New page: '''Unreleased structure''' The entry 5zyx is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 5zyx is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]