6a1s - Revision history

OCA at 10:30, 27 March 2024

OCA — Wed, 27 Mar 2024 10:30:18 GMT

←Older revision		Revision as of 10:30, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6a1s' size='340' side='right'caption='[[6a1s]], [[Resolution\|resolution]] 1.63Å' scene=''>		<StructureSection load='6a1s' size='340' side='right'caption='[[6a1s]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a1s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A1S OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6A1S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a1s]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A1S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A1S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a1s OCA], [~~http~~://pdbe.org/6a1s PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a1s RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a1s PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a1s ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.63Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a1s OCA], [https://pdbe.org/6a1s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a1s RCSB], [https://www.ebi.ac.uk/pdbsum/6a1s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a1s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN]] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>	+	[https://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Charcot-Leyden crystal protein/Gal-10, abundantly expressed in eosinophils and basophils, is related to several immune diseases. Recently, crystallographic and biochemical studies showed that Gal-10 cannot bind lactose, because a glutamate residue (Glu33) from another monomer blocks the binding site. Moreover, Gal-10 actually forms a novel dimeric structure compared to other galectins. To investigate the role that Glu33 plays in inhibiting lactose binding, we mutated this residue to glutamine, aspartate, and alanine. The structure of E33A shows that Gal-10 can now bind lactose. In the hemagglutination assay, lactose could inhibit E33A from inducing chicken erythrocyte agglutination. Furthermore, we identified a tryptophan residue (Trp127) at the interface of homodimer that is crucial for Gal-10 dimerization. The variant W127A, which exists as a monomer, exhibited higher hemagglutination activity than wild type Gal-10. The solid phase assay also showed that W127A could bind to lactose-modified sepharose-6B, whereas wild type Gal-10 could not. This indicates that the open carbohydrate-binding site of the W127A monomer can bind to lactose. In addition, the distribution of EGFP-tagged Gal-10 and its variants in HeLa cells was investigated. Because Trp72 is the highly conserved in the ligand binding sites of galectins, we used EGFP-tagged W72A to show that Gal-10 could not be transported into the nucleus, indicating that Trp72 is crucial for Gal-10 transport into that organelle.	+
-		+
-	Identification of key amino acid residues determining ligand binding specificity, homodimerization and cellular distribution of human galectin-10.,Su J, Song C, Si Y, Cui L, Yang T, Li Y, Wang H, Tai G, Zhou Y Glycobiology. 2019 Jan 1;29(1):85-93. doi: 10.1093/glycob/cwy087. PMID:30239701<ref>PMID:30239701</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6a1s" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
Line 24:		Line 16:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Su, J]]	+	[[Category: Su J]]
-	~~[[Category: Charcot-leyden crystal protein/galectin-10]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 21:25, 28 October 2020

OCA — Wed, 28 Oct 2020 21:25:27 GMT

←Older revision		Revision as of 21:25, 28 October 2020
Line 1:		Line 1:

	==Charcot-Leyden crystal protein/Galectin-10 variant E33A==		==Charcot-Leyden crystal protein/Galectin-10 variant E33A==
-	<StructureSection load='6a1s' size='340' side='right' caption='[[6a1s]], [[Resolution\|resolution]] 1.63Å' scene=''>	+	<StructureSection load='6a1s' size='340' side='right'caption='[[6a1s]], [[Resolution\|resolution]] 1.63Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a1s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A1S OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A1S FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a1s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A1S OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6A1S FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a1s OCA], [http://pdbe.org/6a1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a1s RCSB], [http://www.ebi.ac.uk/pdbsum/6a1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a1s ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a1s OCA], [http://pdbe.org/6a1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a1s RCSB], [http://www.ebi.ac.uk/pdbsum/6a1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a1s ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 17:		Line 17:
	</div>		</div>
	<div class="pdbe-citations 6a1s" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6a1s" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Galectin 3D structures\|Galectin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Su, J]]		[[Category: Su, J]]
	[[Category: Charcot-leyden crystal protein/galectin-10]]		[[Category: Charcot-leyden crystal protein/galectin-10]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 08:12, 26 December 2018

OCA — Wed, 26 Dec 2018 08:12:52 GMT

←Older revision		Revision as of 08:12, 26 December 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6a1s is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Charcot-Leyden crystal protein/Galectin-10 variant E33A==
		+	<StructureSection load='6a1s' size='340' side='right' caption='[[6a1s]], [[Resolution\|resolution]] 1.63Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a1s]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A1S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A1S FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a1s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a1s OCA], [http://pdbe.org/6a1s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a1s RCSB], [http://www.ebi.ac.uk/pdbsum/6a1s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a1s ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LEG10_HUMAN LEG10_HUMAN]] Regulates immune responses through the recognition of cell-surface glycans. Essential for the anergy and suppressive function of CD25-positive regulatory T-cells (Treg).<ref>PMID:17502455</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Charcot-Leyden crystal protein/Gal-10, abundantly expressed in eosinophils and basophils, is related to several immune diseases. Recently, crystallographic and biochemical studies showed that Gal-10 cannot bind lactose, because a glutamate residue (Glu33) from another monomer blocks the binding site. Moreover, Gal-10 actually forms a novel dimeric structure compared to other galectins. To investigate the role that Glu33 plays in inhibiting lactose binding, we mutated this residue to glutamine, aspartate, and alanine. The structure of E33A shows that Gal-10 can now bind lactose. In the hemagglutination assay, lactose could inhibit E33A from inducing chicken erythrocyte agglutination. Furthermore, we identified a tryptophan residue (Trp127) at the interface of homodimer that is crucial for Gal-10 dimerization. The variant W127A, which exists as a monomer, exhibited higher hemagglutination activity than wild type Gal-10. The solid phase assay also showed that W127A could bind to lactose-modified sepharose-6B, whereas wild type Gal-10 could not. This indicates that the open carbohydrate-binding site of the W127A monomer can bind to lactose. In addition, the distribution of EGFP-tagged Gal-10 and its variants in HeLa cells was investigated. Because Trp72 is the highly conserved in the ligand binding sites of galectins, we used EGFP-tagged W72A to show that Gal-10 could not be transported into the nucleus, indicating that Trp72 is crucial for Gal-10 transport into that organelle.

-	~~Authors: Su~~, J.Y.	+	Identification of key amino acid residues determining ligand binding specificity, homodimerization and cellular distribution of human galectin-10.,Su J, Song C, Si Y, Cui L, Yang T, Li Y, Wang H, Tai G, Zhou Y Glycobiology. 2019 Jan 1;29(1):85-93. doi: 10.1093/glycob/cwy087. PMID:30239701<ref>PMID:30239701</ref>

-	~~Description~~: Charcot-~~Leyden~~ crystal protein/~~Galectin~~-10 ~~variant E33A~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[Category: Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Su, J.Y~~]]	+	<div class="pdbe-citations 6a1s" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Su, J]]
		+	[[Category: Charcot-leyden crystal protein/galectin-10]]
		+	[[Category: Protein binding]]

OCA at 16:50, 1 August 2018

OCA — Wed, 01 Aug 2018 16:50:29 GMT

←Older revision		Revision as of 16:50, 1 August 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6a1s is ON HOLD	+	The entry 6a1s is ON HOLD until Paper Publication

	Authors: Su, J.Y.		Authors: Su, J.Y.

OCA: Protected "6a1s" [edit=sysop:move=sysop]

OCA — Thu, 14 Jun 2018 05:32:26 GMT

Protected "6a1s" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 14 June 2018

OCA: New page: '''Unreleased structure''' The entry 6a1s is ON HOLD Authors: Su, J.Y. Description: Charcot-Leyden crystal protein/Galectin-10 variant E33A Category: Unreleased Structures [[Catego...

OCA — Thu, 14 Jun 2018 05:32:25 GMT

New page: '''Unreleased structure''' The entry 6a1s is ON HOLD Authors: Su, J.Y. Description: Charcot-Leyden crystal protein/Galectin-10 variant E33A Category: Unreleased Structures [[Catego...

New page

'''Unreleased structure'''

The entry 6a1s is ON HOLD

Authors: Su, J.Y.

Description: Charcot-Leyden crystal protein/Galectin-10 variant E33A
[[Category: Unreleased Structures]]
[[Category: Su, J.Y]]