6a2i - Revision history

OCA at 09:15, 22 November 2023

OCA — Wed, 22 Nov 2023 09:15:13 GMT

←Older revision		Revision as of 09:15, 22 November 2023
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	<StructureSection load='6a2i' size='340' side='right'caption='[[6a2i]], [[Resolution\|resolution]] 2.40Å' scene=''>		<StructureSection load='6a2i' size='340' side='right'caption='[[6a2i]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a2i]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Sacs2 Sacs2~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a2i]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus_P2 Saccharolobus solfataricus P2] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A2I FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6a2h\|6a2h]]</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">creN7, SSO6901 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=273057 SACS2])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2i OCA], [https://pdbe.org/6a2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a2i RCSB], [https://www.ebi.ac.uk/pdbsum/6a2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2i ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2i OCA], [~~http~~://pdbe.org/6a2i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a2i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a2i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2i ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~CREN7_SULSO CREN7_SULSO]~~] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref>	+	[https://www.uniprot.org/uniprot/CREN7_SACS2 CREN7_SACS2] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Sacs2~~]]	+	[[Category: Saccharolobus solfataricus P2]]
-	[[Category: Chen~~, Y Y~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Dong~~, Y H~~]]	+	[[Category: Chen YY]]
-	[[Category: Gong, Y]]	+	[[Category: Dong YH]]
-	[[Category: Huang, L]]	+	[[Category: Gong Y]]
-	[[Category: Wang, L]]	+	[[Category: Huang L]]
-	[[Category: Zhang~~, Z F~~]]	+	[[Category: Wang L]]
-	[[Category: Zhao~~, M H]]~~	+	[[Category: Zhang ZF]]
-	~~[[Category: Beta-sheet]]~~	+	[[Category: Zhao MH]]
-	~~[[Category: Crenarchaeal chromatin protein]]~~	+
-	~~[[Category: Dna binding protein-dna complex~~]]	+

OCA at 06:56, 27 March 2019

OCA — Wed, 27 Mar 2019 06:56:42 GMT

←Older revision		Revision as of 06:56, 27 March 2019
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	==Architectural roles of Cren7 in folding crenarchaeal chromatin filament==		==Architectural roles of Cren7 in folding crenarchaeal chromatin filament==
-	<StructureSection load='6a2i' size='340' side='right' caption='[[6a2i]], [[Resolution\|resolution]] 2.40Å' scene=''>	+	<StructureSection load='6a2i' size='340' side='right'caption='[[6a2i]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a2i]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a2i]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sacs2 Sacs2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6a2h\|6a2h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6a2h\|6a2h]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">creN7, SSO6901 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=273057 SACS2])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2i OCA], [http://pdbe.org/6a2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2i RCSB], [http://www.ebi.ac.uk/pdbsum/6a2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2i OCA], [http://pdbe.org/6a2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2i RCSB], [http://www.ebi.ac.uk/pdbsum/6a2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2i ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sacs2]]
	[[Category: Chen, Y Y]]		[[Category: Chen, Y Y]]
	[[Category: Dong, Y H]]		[[Category: Dong, Y H]]

OCA at 11:59, 16 January 2019

OCA — Wed, 16 Jan 2019 11:59:21 GMT

←Older revision		Revision as of 11:59, 16 January 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6a2i is ~~ON HOLD~~ ~~until Paper~~ Publication	+	==Architectural roles of Cren7 in folding crenarchaeal chromatin filament==
		+	<StructureSection load='6a2i' size='340' side='right' caption='[[6a2i]], [[Resolution\|resolution]] 2.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a2i]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A2I FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6a2h\|6a2h]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a2i OCA], [http://pdbe.org/6a2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a2i RCSB], [http://www.ebi.ac.uk/pdbsum/6a2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a2i ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CREN7_SULSO CREN7_SULSO]] A highly abundant probable chromatin protein, it binds double-strand DNA without sequence specificity; there is approximately 1 Cren7 molecule for 12 bp of DNA. Constrains negative DNA supercoils, increases DNA stability against thermal denaturation. Binding does not require protein methylation. Binds single-strand DNA weakly.<ref>PMID:18096617</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Archaea have evolved various strategies in chromosomal organization. While histone homologues exist in most archaeal phyla, Cren7 is a chromatin protein conserved in the Crenarchaeota. Here, we show that Cren7 preferentially binds DNA with AT-rich sequences over that with GC-rich sequences with a binding size of 6~7 bp. Structural studies of Cren7 in complex with either an 18-bp or a 20-bp double-stranded DNA fragment reveal that Cren7 binds to the minor groove of DNA as monomers in a head-to-tail manner. The neighboring Cren7 monomers are located on the opposite sides of the DNA duplex, with each introducing a single-step sharp kink by intercalation of the hydrophobic side chain of Leu28, bending the DNA into an S-shape conformation. A structural model for the chromatin fiber folded by Cren7 was established and verified by the analysis of cross-linked Cren7-DNA complexes by atomic force microscopy. Our results suggest that Cren7 differs significantly from Sul7, another chromatin protein conserved among Sulfolobus species, in both DNA binding and deformation. These data shed significant light on the strategy of chromosomal DNA organization in crenarchaea.

-	~~Authors: Zhang~~, Z~~.F.~~, Zhao, M~~.H.~~, Chen, Y~~.Y.~~, Wang, L., Dong, Y~~.H.~~, Gong, Y., Huang, L.	+	Architectural roles of Cren7 in folding crenarchaeal chromatin filament.,Zhang Z, Zhao M, Chen Y, Wang L, Liu Q, Dong Y, Gong Y, Huang L Mol Microbiol. 2018 Nov 30. doi: 10.1111/mmi.14173. PMID:30499242<ref>PMID:30499242</ref>

-	~~Description: Architectural roles~~ of ~~Cren7 in folding crenarchaeal chromatin filament~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6a2i" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Chen, Y Y]]
		+	[[Category: Dong, Y H]]
		+	[[Category: Gong, Y]]
	[[Category: Huang, L]]		[[Category: Huang, L]]
-	[[Category: Chen, Y.Y]]
	[[Category: Wang, L]]		[[Category: Wang, L]]
-	[[Category: Zhao, M.H]]	+	[[Category: Zhang, Z F]]
-	[[Category: ~~Zhang, Z.F~~]]	+	[[Category: Zhao, M H]]
-	[[Category: ~~Dong, Y.H~~]]	+	[[Category: Beta-sheet]]
-	[[Category: ~~Gong, Y~~]]	+	[[Category: Crenarchaeal chromatin protein]]
		+	[[Category: Dna binding protein-dna complex]]

OCA at 06:06, 10 October 2018

OCA — Wed, 10 Oct 2018 06:06:36 GMT

←Older revision		Revision as of 06:06, 10 October 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6a2i is ON HOLD	+	The entry 6a2i is ON HOLD until Paper Publication

	Authors: Zhang, Z.F., Zhao, M.H., Chen, Y.Y., Wang, L., Dong, Y.H., Gong, Y., Huang, L.		Authors: Zhang, Z.F., Zhao, M.H., Chen, Y.Y., Wang, L., Dong, Y.H., Gong, Y., Huang, L.

OCA: Protected "6a2i" [edit=sysop:move=sysop]

OCA — Wed, 20 Jun 2018 03:55:10 GMT

Protected "6a2i" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:55, 20 June 2018

OCA: New page: '''Unreleased structure''' The entry 6a2i is ON HOLD Authors: Zhang, Z.F., Zhao, M.H., Chen, Y.Y., Wang, L., Dong, Y.H., Gong, Y., Huang, L. Description: Architectural roles of Cren7 i...

OCA — Wed, 20 Jun 2018 03:55:08 GMT

New page: '''Unreleased structure''' The entry 6a2i is ON HOLD Authors: Zhang, Z.F., Zhao, M.H., Chen, Y.Y., Wang, L., Dong, Y.H., Gong, Y., Huang, L. Description: Architectural roles of Cren7 i...

New page

'''Unreleased structure'''

The entry 6a2i is ON HOLD

Authors: Zhang, Z.F., Zhao, M.H., Chen, Y.Y., Wang, L., Dong, Y.H., Gong, Y., Huang, L.

Description: Architectural roles of Cren7 in folding crenarchaeal chromatin filament
[[Category: Unreleased Structures]]
[[Category: Huang, L]]
[[Category: Chen, Y.Y]]
[[Category: Wang, L]]
[[Category: Zhao, M.H]]
[[Category: Zhang, Z.F]]
[[Category: Dong, Y.H]]
[[Category: Gong, Y]]