6a52 - Revision history

OCA at 10:30, 27 March 2024

OCA — Wed, 27 Mar 2024 10:30:34 GMT

←Older revision		Revision as of 10:30, 27 March 2024
Line 3:		Line 3:
	<StructureSection load='6a52' size='340' side='right'caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6a52' size='340' side='right'caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Dsm_44812 Dsm 44812~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6A52 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_phenolicus Rhodococcus phenolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A52 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [~~http~~://pdbe.org/6a52 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [https://pdbe.org/6a52 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [https://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/A0A3F2YLV5_9NOCA A0A3F2YLV5_9NOCA]
-	Oxidative rearrangements play key roles in introducing structural complexity and biological activities of natural products biosynthesized by type II polyketide synthases (PKSs). Chartreusin (1) is a potent antitumor polyketide that contains a unique rearranged pentacyclic aromatic bilactone aglycone derived from a type II PKS. Herein, we report an unprecedented dioxygenase, ChaP, that catalyzes the final alpha-pyrone ring formation in 1 biosynthesis using flavin-activated oxygen as an oxidant. The X-ray crystal structures of ChaP and two homologues, docking studies, and site-directed mutagenesis provided insights into the molecular basis of the oxidative rearrangement that involves two successive C-C bond cleavage steps followed by lactonization. ChaP is the first example of a dioxygenase that requires a flavin-activated oxygen as a substrate despite lacking flavin binding sites, and represents a new class in the vicinal oxygen chelate enzyme superfamily.	+
-		+
-	Molecular Basis for the Final Oxidative Rearrangement Steps in Chartreusin Biosynthesis.,Wang YS, Zhang B, Zhu J, Yang CL, Guo Y, Liu CL, Liu F, Huang H, Zhao S, Liang Y, Jiao RH, Tan RX, Ge HM J Am Chem Soc. 2018 Aug 15. doi: ~~10.1021~~/~~jacs.8b06623. PMID:30067334<ref>PMID:30067334<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6a52" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Dsm 44812]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ge, H M~~]]	+	[[Category: Rhodococcus phenolicus]]
-	[[Category: ~~Zhang, B~~]]	+	[[Category: Ge HM]]
-	[[Category: ~~Biosynthetic protein]]~~	+	[[Category: Zhang B]]
-	~~[[Category: Chartreusin]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Dioxygenase]]~~	+
-	~~[[Category: Oxidative rearrangement]]~~	+
-	~~[[Category: Voc family~~]]	+

OCA at 21:26, 28 October 2020

OCA — Wed, 28 Oct 2020 21:26:11 GMT

←Older revision		Revision as of 21:26, 28 October 2020
Line 1:		Line 1:

	==Oxidase ChaP-H1==		==Oxidase ChaP-H1==
-	<StructureSection load='6a52' size='340' side='right' caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='6a52' size='340' side='right'caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_44812 Dsm 44812]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A52 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_44812 Dsm 44812]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6A52 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [http://pdbe.org/6a52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [http://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [http://pdbe.org/6a52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [http://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Dsm 44812]]		[[Category: Dsm 44812]]
		+	[[Category: Large Structures]]
	[[Category: Ge, H M]]		[[Category: Ge, H M]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]

OCA at 20:14, 19 September 2018

OCA — Wed, 19 Sep 2018 20:14:52 GMT

←Older revision		Revision as of 20:14, 19 September 2018
Line 3:		Line 3:
	<StructureSection load='6a52' size='340' side='right' caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6a52' size='340' side='right' caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A52 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_44812 Dsm 44812]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A52 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [http://pdbe.org/6a52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [http://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [http://pdbe.org/6a52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [http://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Dsm 44812]]
	[[Category: Ge, H M]]		[[Category: Ge, H M]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]

OCA at 07:22, 29 August 2018

OCA — Wed, 29 Aug 2018 07:22:58 GMT

←Older revision		Revision as of 07:22, 29 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6a52 is ~~ON HOLD until Paper~~ Publication	+	==Oxidase ChaP-H1==
		+	<StructureSection load='6a52' size='340' side='right' caption='[[6a52]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a52]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A52 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A52 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a52 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a52 OCA], [http://pdbe.org/6a52 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a52 RCSB], [http://www.ebi.ac.uk/pdbsum/6a52 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a52 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Oxidative rearrangements play key roles in introducing structural complexity and biological activities of natural products biosynthesized by type II polyketide synthases (PKSs). Chartreusin (1) is a potent antitumor polyketide that contains a unique rearranged pentacyclic aromatic bilactone aglycone derived from a type II PKS. Herein, we report an unprecedented dioxygenase, ChaP, that catalyzes the final alpha-pyrone ring formation in 1 biosynthesis using flavin-activated oxygen as an oxidant. The X-ray crystal structures of ChaP and two homologues, docking studies, and site-directed mutagenesis provided insights into the molecular basis of the oxidative rearrangement that involves two successive C-C bond cleavage steps followed by lactonization. ChaP is the first example of a dioxygenase that requires a flavin-activated oxygen as a substrate despite lacking flavin binding sites, and represents a new class in the vicinal oxygen chelate enzyme superfamily.

-	~~Authors: Zhang~~, B., Ge, H.M.	+	Molecular Basis for the Final Oxidative Rearrangement Steps in Chartreusin Biosynthesis.,Wang YS, Zhang B, Zhu J, Yang CL, Guo Y, Liu CL, Liu F, Huang H, Zhao S, Liang Y, Jiao RH, Tan RX, Ge HM J Am Chem Soc. 2018 Aug 15. doi: 10.1021/jacs.8b06623. PMID:30067334<ref>PMID:30067334</ref>

-	~~Description: Oxidase ChaP~~-H1	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6a52" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Ge, H M]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]
-	[[Category: ~~Ge, H.M~~]]	+	[[Category: Biosynthetic protein]]
		+	[[Category: Chartreusin]]
		+	[[Category: Dimer]]
		+	[[Category: Dioxygenase]]
		+	[[Category: Oxidative rearrangement]]
		+	[[Category: Voc family]]

OCA at 03:55, 11 July 2018

OCA — Wed, 11 Jul 2018 03:55:19 GMT

←Older revision		Revision as of 03:55, 11 July 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6a52 is ON HOLD	+	The entry 6a52 is ON HOLD until Paper Publication

	Authors: Zhang, B., Ge, H.M.		Authors: Zhang, B., Ge, H.M.

OCA at 05:31, 4 July 2018

OCA — Wed, 04 Jul 2018 05:31:42 GMT

←Older revision		Revision as of 05:31, 4 July 2018
Line 5:		Line 5:
	Authors: Zhang, B., Ge, H.M.		Authors: Zhang, B., Ge, H.M.

-	Description: ~~oxidase~~ ChaP-H1	+	Description: Oxidase ChaP-H1
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]
	[[Category: Ge, H.M]]		[[Category: Ge, H.M]]

OCA: Protected "6a52" [edit=sysop:move=sysop]

OCA — Wed, 27 Jun 2018 03:55:49 GMT

Protected "6a52" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:55, 27 June 2018

OCA: New page: '''Unreleased structure''' The entry 6a52 is ON HOLD Authors: Zhang, B., Ge, H.M. Description: oxidase ChaP-H1 Category: Unreleased Structures Category: Zhang, B [[Category: Ge...

OCA — Wed, 27 Jun 2018 03:55:48 GMT

New page: '''Unreleased structure''' The entry 6a52 is ON HOLD Authors: Zhang, B., Ge, H.M. Description: oxidase ChaP-H1 Category: Unreleased Structures Category: Zhang, B [[Category: Ge...

New page

'''Unreleased structure'''

The entry 6a52 is ON HOLD

Authors: Zhang, B., Ge, H.M.

Description: oxidase ChaP-H1
[[Category: Unreleased Structures]]
[[Category: Zhang, B]]
[[Category: Ge, H.M]]