6a5h - Revision history

OCA at 09:18, 22 November 2023

OCA — Wed, 22 Nov 2023 09:18:52 GMT

←Older revision		Revision as of 09:18, 22 November 2023
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	<StructureSection load='6a5h' size='340' side='right'caption='[[6a5h]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='6a5h' size='340' side='right'caption='[[6a5h]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Nocardia_tenerifensis_nbrc_101015~~ Nocardia tenerifensis ~~nbrc~~ 101015]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A5H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_tenerifensis_NBRC_101015 Nocardia tenerifensis NBRC 101015]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A5H FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a5h OCA], [https://pdbe.org/6a5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a5h RCSB], [https://www.ebi.ac.uk/pdbsum/6a5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a5h ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.618Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a5h OCA], [https://pdbe.org/6a5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a5h RCSB], [https://www.ebi.ac.uk/pdbsum/6a5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a5h ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nocardia tenerifensis ~~nbrc~~ 101015]]	+	[[Category: Nocardia tenerifensis NBRC 101015]]
-	[[Category: Ge~~, H M~~]]	+	[[Category: Ge HM]]
-	[[Category: Zhang, B]]	+	[[Category: Zhang B]]
-	~~[[Category: Biosynthesis]]~~	+
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Natural product~~]]	+

OCA at 10:14, 18 March 2021

OCA — Thu, 18 Mar 2021 10:14:14 GMT

←Older revision		Revision as of 10:14, 18 March 2021
Line 3:		Line 3:
	<StructureSection load='6a5h' size='340' side='right'caption='[[6a5h]], [[Resolution\|resolution]] 1.62Å' scene=''>		<StructureSection load='6a5h' size='340' side='right'caption='[[6a5h]], [[Resolution\|resolution]] 1.62Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a5h]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Nocardia_tenerifensis_nbrc_101015 Nocardia tenerifensis nbrc 101015]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A5H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A5H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a5h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nocardia_tenerifensis_nbrc_101015 Nocardia tenerifensis nbrc 101015]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A5H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A5H FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a5h OCA], [~~http~~://pdbe.org/6a5h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a5h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a5h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a5h ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a5h OCA], [https://pdbe.org/6a5h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a5h RCSB], [https://www.ebi.ac.uk/pdbsum/6a5h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a5h ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:42, 10 April 2019

OCA — Wed, 10 Apr 2019 07:42:59 GMT

←Older revision		Revision as of 07:42, 10 April 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6a5h is ~~ON HOLD until Paper~~ Publication	+	==The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of unidentified natural product==
		+	<StructureSection load='6a5h' size='340' side='right'caption='[[6a5h]], [[Resolution\|resolution]] 1.62Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a5h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Nocardia_tenerifensis_nbrc_101015 Nocardia tenerifensis nbrc 101015]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A5H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A5H FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a5h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a5h OCA], [http://pdbe.org/6a5h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a5h RCSB], [http://www.ebi.ac.uk/pdbsum/6a5h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a5h ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pericyclic reactions are powerful transformations for the construction of carbon-carbon and carbon-heteroatom bonds in organic synthesis. Their role in biosynthesis is increasingly apparent, and mechanisms by which pericyclases can catalyse reactions are of major interest(1). [4+2] cycloadditions (Diels-Alder reactions) have been widely used in organic synthesis(2) for the formation of six-membered rings and are now well-established in biosynthesis(3-6). [6+4] and other 'higher-order' cycloadditions were predicted(7) in 1965, and are now increasingly common in the laboratory despite challenges arising from the generation of a highly strained ten-membered ring system(8,9). However, although enzyme-catalysed [6+4] cycloadditions have been proposed(10-12), they have not been proven to occur. Here we demonstrate a group of enzymes that catalyse a pericyclic [6+4] cycloaddition, which is a crucial step in the biosynthesis of streptoseomycin-type natural products. This type of pericyclase catalyses [6+4] and [4+2] cycloadditions through a single ambimodal transition state, which is consistent with previous proposals(11,12). The [6+4] product is transformed to a less stable [4+2] adduct via a facile Cope rearrangement, and the [4+2] adduct is converted into the natural product enzymatically. Crystal structures of three pericyclases, computational simulations of potential energies and molecular dynamics, and site-directed mutagenesis establish the mechanism of this transformation. This work shows how enzymes are able to catalyse concerted pericyclic reactions involving ambimodal transition states.

-	~~Authors: Zhang~~, B., Ge, H.M.	+	Enzyme-catalysed [6+4] cycloadditions in the biosynthesis of natural products.,Zhang B, Wang KB, Wang W, Wang X, Liu F, Zhu J, Shi J, Li LY, Han H, Xu K, Qiao HY, Zhang X, Jiao RH, Houk KN, Liang Y, Tan RX, Ge HM Nature. 2019 Apr;568(7750):122-126. doi: 10.1038/s41586-019-1021-x. Epub 2019 Mar, 13. PMID:30867595<ref>PMID:30867595</ref>

-	~~Description: The structure~~ of [~~4+2~~] ~~and~~ [~~6+4~~] ~~cyclase in the biosynthetic pathway of unidentified natural product~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6a5h" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nocardia tenerifensis nbrc 101015]]
		+	[[Category: Ge, H M]]
	[[Category: Zhang, B]]		[[Category: Zhang, B]]
-	[[Category: ~~Ge, H.M~~]]	+	[[Category: Biosynthesis]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Natural product]]

OCA: Protected "6a5h" [edit=sysop:move=sysop]

OCA — Wed, 04 Jul 2018 05:32:13 GMT

Protected "6a5h" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:32, 4 July 2018

OCA: New page: '''Unreleased structure''' The entry 6a5h is ON HOLD until Paper Publication Authors: Zhang, B., Ge, H.M. Description: The structure of [4+2] and [6+4] cyclase in the biosynthetic path...

OCA — Wed, 04 Jul 2018 05:32:12 GMT

New page: '''Unreleased structure''' The entry 6a5h is ON HOLD until Paper Publication Authors: Zhang, B., Ge, H.M. Description: The structure of [4+2] and [6+4] cyclase in the biosynthetic path...

New page

'''Unreleased structure'''

The entry 6a5h is ON HOLD until Paper Publication

Authors: Zhang, B., Ge, H.M.

Description: The structure of [4+2] and [6+4] cyclase in the biosynthetic pathway of unidentified natural product
[[Category: Unreleased Structures]]
[[Category: Zhang, B]]
[[Category: Ge, H.M]]