6a80 - Revision history

OCA at 09:20, 22 November 2023

OCA — Wed, 22 Nov 2023 09:20:48 GMT

←Older revision		Revision as of 09:20, 22 November 2023
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	<StructureSection load='6a80' size='340' side='right'caption='[[6a80]], [[Resolution\|resolution]] 1.56Å' scene=''>		<StructureSection load='6a80' size='340' side='right'caption='[[6a80]], [[Resolution\|resolution]] 1.56Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6a80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A80 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6A80 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6a80]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Liberibacter_asiaticus_str._psy62 Candidatus Liberibacter asiaticus str. psy62]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A80 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6A80 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.56Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca.weizmann~~.~~ac.il/oca-docs~~/fgij/fg.htm?mol=6a80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a80 OCA], [~~http~~://pdbe.org/6a80 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6a80 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6a80 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6a80 ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6a80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a80 OCA], [https://pdbe.org/6a80 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6a80 RCSB], [https://www.ebi.ac.uk/pdbsum/6a80 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6a80 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/C6XGT2_LIBAP C6XGT2_LIBAP]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Candidatus Liberibacter asiaticus str. psy62]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ghosh~~, D K~~]]	+	[[Category: Ghosh DK]]
-	[[Category: Kesari, P]]	+	[[Category: Kesari P]]
-	[[Category: Kumar, P]]	+	[[Category: Kumar P]]
-	[[Category: Sharma~~, A K]]~~	+	[[Category: Sharma AK]]
-	~~[[Category: Candidatus liberibacter asiaticus]]~~	+
-	~~[[Category: Periplasmic domain]]~~	+
-	~~[[Category: Putative amino acid binding]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 05:26, 12 June 2019

OCA — Wed, 12 Jun 2019 05:26:10 GMT

←Older revision		Revision as of 05:26, 12 June 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6a80 is ~~ON HOLD until Paper~~ Publication	+	==Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine==
		+	<StructureSection load='6a80' size='340' side='right'caption='[[6a80]], [[Resolution\|resolution]] 1.56Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6a80]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6A80 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6A80 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CYS:CYSTEINE'>CYS</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6a80 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6a80 OCA], [http://pdbe.org/6a80 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6a80 RCSB], [http://www.ebi.ac.uk/pdbsum/6a80 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6a80 ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The amino acid-binding receptors, a component of ABC transporters, have evolved to cater to different specificities and functions. Of particular interest are cystine-binding receptors, which have shown broad specificity. In the present study, a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus (CLasTcyA) was characterized. Analysis of the CLasTcyA sequence and crystal structures in the ligand-bound state revealed novel features of CLasTcyA in comparison to related proteins. One of the unique features found in CLasTcyA structure was the positioning of the C-terminal extended loop of one chain very close to the substrate-binding site of the adjacent monomer in the asymmetric unit. The presence of a disulphide bond, unique to Candidatus Liberibacter family, holds the C-terminal extended loop in position. Analysis of the substrate-binding pocket of CLasTcyA suggested a broad specificity and a completely different orientation of the bound substrates in comparison to related protein structures. The open conformation for one of the two chains of the asymmetric unit in the Arg-bound structure revealed a limited open state (18.4 degrees ) for CLasTcyA as compared to open state of other related proteins (~ 60 degrees ). The strong interaction between Asp126 on helix-alpha5 of small domain and Arg82 (bigger domain) restricts the degree of opening in ligand-free open state. The dissociation constant of 1.26 mum by SPR and 3.7 mum by MST exhibited low affinity for the cystine. This is the first structural characterization of an l-cystine ABC transporter from plant pathogen and our results suggest that CLasTcyA may have evolved to cater to its specific needs for its survival in the host.

-	~~Authors~~: Kumar, P., Kesari, P., Ghosh~~, D.K.~~, Kumar, P., Sharma~~, A~~.K.	+	Crystal structures of a putative periplasmic cystine-binding protein from Candidatus Liberibacter asiaticus: insights into an adapted mechanism of ligand binding.,Kumar P, Kesari P, Kokane S, Ghosh DK, Kumar P, Sharma AK FEBS J. 2019 May 7. doi: 10.1111/febs.14921. PMID:31063259<ref>PMID:31063259</ref>

-	~~Description: Crystal Structure~~ of ~~putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6a80" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Ghosh, D K]]
	[[Category: Kesari, P]]		[[Category: Kesari, P]]
	[[Category: Kumar, P]]		[[Category: Kumar, P]]
-	[[Category: ~~Ghosh~~, D.K]]	+	[[Category: Sharma, A K]]
-	[[Category: ~~Sharma, A.K~~]]	+	[[Category: Candidatus liberibacter asiaticus]]
		+	[[Category: Periplasmic domain]]
		+	[[Category: Putative amino acid binding]]
		+	[[Category: Transport protein]]

OCA at 19:40, 9 August 2018

OCA — Thu, 09 Aug 2018 19:40:09 GMT

←Older revision		Revision as of 19:40, 9 August 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6a80 is ON HOLD	+	The entry 6a80 is ON HOLD until Paper Publication

	Authors: Kumar, P., Kesari, P., Ghosh, D.K., Kumar, P., Sharma, A.K.		Authors: Kumar, P., Kesari, P., Ghosh, D.K., Kumar, P., Sharma, A.K.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Kesari, P]]		[[Category: Kesari, P]]
		+	[[Category: Kumar, P]]
	[[Category: Ghosh, D.K]]		[[Category: Ghosh, D.K]]
	[[Category: Sharma, A.K]]		[[Category: Sharma, A.K]]
-	[[Category: Kumar, P]]

OCA: Protected "6a80" [edit=sysop:move=sysop]

OCA — Wed, 11 Jul 2018 03:59:11 GMT

Protected "6a80" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:59, 11 July 2018

OCA: New page: '''Unreleased structure''' The entry 6a80 is ON HOLD Authors: Kumar, P., Kesari, P., Ghosh, D.K., Kumar, P., Sharma, A.K. Description: Crystal Structure of putative amino acid binding ...

OCA — Wed, 11 Jul 2018 03:59:10 GMT

New page: '''Unreleased structure''' The entry 6a80 is ON HOLD Authors: Kumar, P., Kesari, P., Ghosh, D.K., Kumar, P., Sharma, A.K. Description: Crystal Structure of putative amino acid binding ...

New page

'''Unreleased structure'''

The entry 6a80 is ON HOLD

Authors: Kumar, P., Kesari, P., Ghosh, D.K., Kumar, P., Sharma, A.K.

Description: Crystal Structure of putative amino acid binding periplasmic ABC transporter protein from Candidatus Liberibacter asiaticus in complex with cystine
[[Category: Unreleased Structures]]
[[Category: Kesari, P]]
[[Category: Ghosh, D.K]]
[[Category: Sharma, A.K]]
[[Category: Kumar, P]]