6aby - Revision history

OCA at 09:24, 22 November 2023

OCA — Wed, 22 Nov 2023 09:24:34 GMT

←Older revision		Revision as of 09:24, 22 November 2023
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	<StructureSection load='6aby' size='340' side='right'caption='[[6aby]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6aby' size='340' side='right'caption='[[6aby]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aby]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6ABY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aby]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Metallosphaera_sedula_DSM_5348 Metallosphaera sedula DSM 5348]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ABY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_synthase_(unknown_stereospecificity) Citrate synthase (unknown stereospecificity)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.16 2.3.3.16] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6aby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aby OCA], [~~http~~://pdbe.org/6aby PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6aby RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6aby PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6aby ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aby OCA], [https://pdbe.org/6aby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aby RCSB], [https://www.ebi.ac.uk/pdbsum/6aby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aby ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A4YGX6_METS5 A4YGX6_METS5]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6aby" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6aby" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Citrate Synthase 3D structures\|Citrate Synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kim, K J]]	+	[[Category: Metallosphaera sedula DSM 5348]]
-	[[Category: Lee, S H]]	+	[[Category: Kim K-J]]
-	[[Category: Son, H F]]	+	[[Category: Lee S-H]]
-	~~[[Category: Citrate synthase]]~~	+	[[Category: Son H-F]]
-	~~[[Category: Metallosphaera sedula]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 07:43, 20 March 2019

OCA — Wed, 20 Mar 2019 07:43:53 GMT

←Older revision		Revision as of 07:43, 20 March 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6aby is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate==
		+	<StructureSection load='6aby' size='340' side='right'caption='[[6aby]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6aby]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ABY FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=OAA:OXALOACETATE+ION'>OAA</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_synthase_(unknown_stereospecificity) Citrate synthase (unknown stereospecificity)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.16 2.3.3.16] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aby OCA], [http://pdbe.org/6aby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aby RCSB], [http://www.ebi.ac.uk/pdbsum/6aby PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aby ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 A resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition.

-	~~Authors: Lee, S.-H~~., Son, H.-F.~~, Kim, K~~.-J.	+	Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.,Lee SH, Son HF, Kim KJ PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807., eCollection 2019. PMID:30794680<ref>PMID:30794680</ref>

-	~~Description: Crystal structure~~ of ~~citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Kim, K.-J]]	+	<div class="pdbe-citations 6aby" style="background-color:#fffaf0;"></div>
-	[[Category: Lee, S.-H]]	+	== References ==
-	[[Category: Son, H.-F]]	+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kim, K J]]
		+	[[Category: Lee, S H]]
		+	[[Category: Son, H F]]
		+	[[Category: Citrate synthase]]
		+	[[Category: Metallosphaera sedula]]
		+	[[Category: Transferase]]

OCA at 07:06, 13 February 2019

OCA — Wed, 13 Feb 2019 07:06:30 GMT

←Older revision		Revision as of 07:06, 13 February 2019
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6aby is ON HOLD	+	The entry 6aby is ON HOLD until Paper Publication

	Authors: Lee, S.-H., Son, H.-F., Kim, K.-J.		Authors: Lee, S.-H., Son, H.-F., Kim, K.-J.

OCA: Protected "6aby" [edit=sysop:move=sysop]

OCA — Wed, 01 Aug 2018 16:54:46 GMT

Protected "6aby" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:54, 1 August 2018

OCA: New page: '''Unreleased structure''' The entry 6aby is ON HOLD Authors: Lee, S.-H., Son, H.-F., Kim, K.-J. Description: Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedu...

OCA — Wed, 01 Aug 2018 16:54:45 GMT

New page: '''Unreleased structure''' The entry 6aby is ON HOLD Authors: Lee, S.-H., Son, H.-F., Kim, K.-J. Description: Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedu...

New page

'''Unreleased structure'''

The entry 6aby is ON HOLD

Authors: Lee, S.-H., Son, H.-F., Kim, K.-J.

Description: Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with oxaloacetate
[[Category: Unreleased Structures]]
[[Category: Kim, K.-J]]
[[Category: Lee, S.-H]]
[[Category: Son, H.-F]]