6akj - Revision history

OCA at 09:35, 22 November 2023

OCA — Wed, 22 Nov 2023 09:35:57 GMT

←Older revision		Revision as of 09:35, 22 November 2023
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	==The crystal structure of EMC complex==		==The crystal structure of EMC complex==
-	<StructureSection load='6akj' size='340' side='right' caption='[[6akj]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='6akj' size='340' side='right'caption='[[6akj]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6akj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AKJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6akj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe_972h- Schizosaccharomyces pombe 972h-]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AKJ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6akj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akj OCA], [~~http~~://pdbe.org/6akj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6akj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6akj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6akj ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6akj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akj OCA], [https://pdbe.org/6akj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6akj RCSB], [https://www.ebi.ac.uk/pdbsum/6akj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6akj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MMI1_SCHPO MMI1_SCHPO] RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, a modification present at internal sites of mRNAs and some non-coding RNAs (By similarity). Required for elemination of certain meiosis-specific mRNAs in an early event following transcription. May bind to the cis-acting region (DSR) of the mRNA, activating the nuclear exosome which may lead to the degradation of the transcript from the 3' region (PubMed:16823445).[UniProtKB:Q06390]<ref>PMID:16823445</ref> [https://www.uniprot.org/uniprot/ERH_SCHPO ERH_SCHPO] Forms part of the erh1-mmi1 complex that recruits the CCR4-NOT complex and the NURS complex to target RNAs (PubMed:26942678, PubMed:30651569, PubMed:31974447). Suppresses the meiotic program during vegetative growth and promotes the meiotic program during mating (PubMed:31974447). Recruitment of the NURS complex to target mRNAs promotes mRNA decay by engagement of the nuclear exosome, and formation of heterochromatin islands at meiotic genes silenced by the exosome (PubMed:26942678). Recruitment of the CCR4-NOT complex to target RNAs promotes heterochromatin formation at RNAi-dependent heterochromatin domains (HOODs), including a subset of meiotic genes, lncRNAs and retrotransposons (PubMed:26942678). Recruitment of the CCR4-NOT complex to rDNA promotes rDNA heterochromatin assembly (PubMed:26942678).<ref>PMID:26942678</ref> <ref>PMID:30651569</ref> <ref>PMID:31974447</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Li, F~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Protein~~-~~protein interaction~~]]	+	[[Category: Schizosaccharomyces pombe 972h-]]
-	[[Category: ~~Rna binding protein~~]]	+	[[Category: Li F]]

OCA at 09:25, 13 February 2019

OCA — Wed, 13 Feb 2019 09:25:38 GMT

←Older revision		Revision as of 09:25, 13 February 2019
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-	'''Unreleased structure'''

-	The ~~entry~~ 6akj is ~~ON HOLD until Sep 01 2020~~	+	==The crystal structure of EMC complex==
		+	<StructureSection load='6akj' size='340' side='right' caption='[[6akj]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6akj]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AKJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AKJ FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6akj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6akj OCA], [http://pdbe.org/6akj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6akj RCSB], [http://www.ebi.ac.uk/pdbsum/6akj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6akj ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Gene regulatory mechanisms rely on a complex network of RNA processing factors to prevent untimely gene expression. In fission yeast, the highly conserved ortholog of human ERH, called Erh1, interacts with the YTH family RNA binding protein Mmi1 to form the Erh1-Mmi1 complex (EMC) implicated in gametogenic gene silencing. However, the structural basis of EMC assembly and its functions are poorly understood. Here, we present the co-crystal structure of the EMC that consists of Erh1 homodimers interacting with Mmi1 in a 2:2 stoichiometry via a conserved molecular interface. Structure-guided mutation of the Mmi1(Trp112) residue, which is required for Erh1 binding, causes defects in facultative heterochromatin assembly and gene silencing while leaving Mmi1-mediated transcription termination intact. Indeed, EMC targets masked in mmi1 due to termination defects are revealed in mmi1(W112A). Our study delineates EMC requirements in gene silencing and identifies an ERH interface required for interaction with an RNA binding protein.

-	~~Authors~~:	+	A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing.,Xie G, Vo TV, Thillainadesan G, Holla S, Zhang B, Jiang Y, Lv M, Xu Z, Wang C, Balachandran V, Shi Y, Li F, Grewal SIS Nat Commun. 2019 Jan 16;10(1):251. doi: 10.1038/s41467-018-08273-9. PMID:30651569<ref>PMID:30651569</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6akj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Li, F]]
		+	[[Category: Protein-protein interaction]]
		+	[[Category: Rna binding protein]]

OCA at 06:05, 26 September 2018

OCA — Wed, 26 Sep 2018 06:05:21 GMT

←Older revision		Revision as of 06:05, 26 September 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6akj is ON HOLD	+	The entry 6akj is ON HOLD until Sep 01 2020

	Authors:		Authors:

OCA: Protected "6akj" [edit=sysop:move=sysop]

OCA — Wed, 12 Sep 2018 05:55:15 GMT

Protected "6akj" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:55, 12 September 2018

OCA: New page: '''Unreleased structure''' The entry 6akj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 12 Sep 2018 05:55:13 GMT

New page: '''Unreleased structure''' The entry 6akj is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6akj is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]