6amq - Revision history

OCA at 14:20, 4 October 2023

OCA — Wed, 04 Oct 2023 14:20:59 GMT

←Older revision		Revision as of 14:20, 4 October 2023
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	<StructureSection load='6amq' size='340' side='right'caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>		<StructureSection load='6amq' size='340' side='right'caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enterobacter_cloacae_ecwsu1~~ Enterobacter cloacae ~~ecwsu1~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacter_cloacae_EcWSU1 Enterobacter cloacae EcWSU1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.67Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ams\|6ams]]~~, [[~~6ap4~~\|~~6ap4~~]]</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~dnaN, EcWSU1_00002 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1045856 Enterobacter cloacae EcWSU1])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amq OCA], [https://pdbe.org/6amq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6amq RCSB], [https://www.ebi.ac.uk/pdbsum/6amq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6amq ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amq OCA], [~~http~~://pdbe.org/6amq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6amq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6amq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6amq ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~G8LES0_ENTCL G8LES0_ENTCL]~~] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]	+	[https://www.uniprot.org/uniprot/G8LES0_9ENTR G8LES0_9ENTR] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Enterobacter cloacae ~~ecwsu1~~]]	+	[[Category: Enterobacter cloacae EcWSU1]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: McGrath~~, A E~~]]	+	[[Category: McGrath AE]]
-	[[Category: Oakley~~, A J]]~~	+	[[Category: Oakley AJ]]
-	~~[[Category: Dna binding]]~~	+
-	~~[[Category: Dna directed dna polymerase activity]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 10:03, 1 January 2020

OCA — Wed, 01 Jan 2020 10:03:48 GMT

←Older revision		Revision as of 10:03, 1 January 2020
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	==Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloacae==		==Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloacae==
-	<StructureSection load='6amq' size='340' side='right' caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>	+	<StructureSection load='6amq' size='340' side='right'caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae_ecwsu1 Enterobacter cloacae ecwsu1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae_ecwsu1 Enterobacter cloacae ecwsu1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>
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	==See Also==		==See Also==
-	*[[DNA polymerase\|DNA polymerase]]	+	*[[DNA polymerase 3D structures\|DNA polymerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Enterobacter cloacae ecwsu1]]		[[Category: Enterobacter cloacae ecwsu1]]
		+	[[Category: Large Structures]]
	[[Category: McGrath, A E]]		[[Category: McGrath, A E]]
	[[Category: Oakley, A J]]		[[Category: Oakley, A J]]

OCA at 12:37, 7 November 2018

OCA — Wed, 07 Nov 2018 12:37:35 GMT

←Older revision		Revision as of 12:37, 7 November 2018
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/G8LES0_ENTCL G8LES0_ENTCL]] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]		[[http://www.uniprot.org/uniprot/G8LES0_ENTCL G8LES0_ENTCL]] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial sliding clamps bind to DNA and act as protein-protein interaction hubs for several proteins involved in DNA replication and repair. The partner proteins all bind to a common pocket on sliding clamps via conserved linear peptide sequence motifs, which suggest the pocket as an attractive target for development of new antibiotics. Herein we report the X-ray crystal structures and biochemical characterization of beta sliding clamps from the Gram-negative pathogens Pseudomonas aeruginosa, Acinetobacter baumannii and Enterobacter cloacae. The structures reveal close similarity between the pathogen and Escherichia coli clamps and similar patterns of binding to linear clamp-binding motif peptides. The results suggest that linear motif-sliding clamp interactions are well conserved and an antibiotic targeting the sliding clamp should have broad-spectrum activity against Gram-negative pathogens.
		+
		+	Crystal structures and biochemical characterization of DNA sliding clamps from three Gram-negative bacterial pathogens.,McGrath AE, Martyn AP, Whittell LR, Dawes FE, Beck JL, Dixon NE, Kelso MJ, Oakley AJ J Struct Biol. 2018 Oct 23. pii: S1047-8477(18)30281-8. doi:, 10.1016/j.jsb.2018.10.008. PMID:30366028<ref>PMID:30366028</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6amq" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[DNA polymerase\|DNA polymerase]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:58, 6 December 2017

OCA — Wed, 06 Dec 2017 07:58:35 GMT

←Older revision		Revision as of 07:58, 6 December 2017
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	<StructureSection load='6amq' size='340' side='right' caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>		<StructureSection load='6amq' size='340' side='right' caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Enterobacter_cloacae_ecwsu1 Enterobacter cloacae ecwsu1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ams\|6ams]], [[6ap4\|6ap4]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ams\|6ams]], [[6ap4\|6ap4]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">dnaN, EcWSU1_00002 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1045856 Enterobacter cloacae EcWSU1])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amq OCA], [http://pdbe.org/6amq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6amq RCSB], [http://www.ebi.ac.uk/pdbsum/6amq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6amq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amq OCA], [http://pdbe.org/6amq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6amq RCSB], [http://www.ebi.ac.uk/pdbsum/6amq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6amq ProSAT]</span></td></tr>
	</table>		</table>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Enterobacter cloacae ecwsu1]]
	[[Category: McGrath, A E]]		[[Category: McGrath, A E]]
	[[Category: Oakley, A J]]		[[Category: Oakley, A J]]

OCA at 06:33, 1 November 2017

OCA — Wed, 01 Nov 2017 06:33:50 GMT

←Older revision		Revision as of 06:33, 1 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6amq is ~~ON HOLD~~	+	==Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloacae==
-		+	<StructureSection load='6amq' size='340' side='right' caption='[[6amq]], [[Resolution\|resolution]] 2.67Å' scene=''>
-	~~Authors~~: ~~McGrath~~, A.E., ~~Oakley~~, A.J.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6amq]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AMQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AMQ FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of~~ the DNA polymerase III ~~subunit~~ beta ~~from Enterobacter cloacae~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	[~~[Category~~: ~~Unreleased Structures]~~]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6ams\|6ams]], [[6ap4\|6ap4]]</td></tr>
-	[[Category: ~~Mcgrath~~, A.E]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6amq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6amq OCA], [http://pdbe.org/6amq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6amq RCSB], [http://www.ebi.ac.uk/pdbsum/6amq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6amq ProSAT]</span></td></tr>
-	[[Category: Oakley, A.J]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/G8LES0_ENTCL G8LES0_ENTCL]] Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication.[PIRNR:PIRNR000804]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: McGrath, A E]]
		+	[[Category: Oakley, A J]]
		+	[[Category: Dna binding]]
		+	[[Category: Dna directed dna polymerase activity]]
		+	[[Category: Transferase]]

OCA: Protected "6amq" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:34:22 GMT

Protected "6amq" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:34, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6amq is ON HOLD Authors: McGrath, A.E., Oakley, A.J. Description: Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloaca...

OCA — Wed, 27 Sep 2017 16:34:21 GMT

New page: '''Unreleased structure''' The entry 6amq is ON HOLD Authors: McGrath, A.E., Oakley, A.J. Description: Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloaca...

New page

'''Unreleased structure'''

The entry 6amq is ON HOLD

Authors: McGrath, A.E., Oakley, A.J.

Description: Crystal structure of the DNA polymerase III subunit beta from Enterobacter cloacae
[[Category: Unreleased Structures]]
[[Category: Mcgrath, A.E]]
[[Category: Oakley, A.J]]