6ana - Revision history

OCA at 07:03, 16 October 2019

OCA — Wed, 16 Oct 2019 07:03:14 GMT

←Older revision		Revision as of 07:03, 16 October 2019
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	==LL2 Fab in complex with anti-Kappa VHH domain==		==LL2 Fab in complex with anti-Kappa VHH domain==
-	<StructureSection load='6ana' size='340' side='right' caption='[[6ana]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='6ana' size='340' side='right'caption='[[6ana]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6ana]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANA FirstGlance]. <br>		<table><tr><td colspan='2'>[[6ana]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANA FirstGlance]. <br>
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	</div>		</div>
	<div class="pdbe-citations 6ana" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6ana" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Camelus glama]]		[[Category: Camelus glama]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Julien, J P]]		[[Category: Julien, J P]]

OCA at 07:02, 15 February 2018

OCA — Thu, 15 Feb 2018 07:02:33 GMT

←Older revision		Revision as of 07:02, 15 February 2018
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	<StructureSection load='6ana' size='340' side='right' caption='[[6ana]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='6ana' size='340' side='right' caption='[[6ana]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6ana]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6ana]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Camelus glama]]
		+	[[Category: Lk3 transgenic mice]]
	[[Category: Julien, J P]]		[[Category: Julien, J P]]
	[[Category: Orbea, J Ereno]]		[[Category: Orbea, J Ereno]]

OCA at 05:56, 31 January 2018

OCA — Wed, 31 Jan 2018 05:56:42 GMT

←Older revision		Revision as of 05:56, 31 January 2018
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-	'''Unreleased structure'''

-	~~The entry~~ 6ana is ~~ON HOLD~~	+	==LL2 Fab in complex with anti-Kappa VHH domain==
		+	<StructureSection load='6ana' size='340' side='right' caption='[[6ana]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6ana]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ANA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ANA FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ana FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ana OCA], [http://pdbe.org/6ana PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ana RCSB], [http://www.ebi.ac.uk/pdbsum/6ana PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ana ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Monoclonal antibodies constitute one of the largest groups of drugs to treat cancers and immune disorders, and are guiding the design of vaccines against infectious diseases. Fragments antigen-binding (Fabs) have been preferred over monoclonal antibodies for the structural characterization of antibody-antigen complexes due to their relatively low flexibility. Nonetheless, Fabs often remain challenging to crystallize because of the surface characteristics of complementary determining regions and the residual flexibility in the hinge region between the variable and constant domains. Here, we used a variable heavy-chain (VHH) domain specific for the human kappa light chain to assist in the structure determination of three therapeutic Fabs that were recalcitrant to crystallization on their own. We show that this ligand alters the surface properties of the antibody-ligand complex and lowers its aggregation temperature to favor crystallization. The VHH crystallization chaperone also restricts the flexible hinge of Fabs to a narrow range of angles, and so independently of the variable region. Our findings contribute a valuable approach to antibody structure determination and provide biophysical insight into the principles that govern the crystallization of macromolecules.

-	~~Authors~~:	+	Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments.,Ereno-Orbea J, Sicard T, Cui H, Carson J, Hermans P, Julien JP J Mol Biol. 2017 Dec 22. pii: S0022-2836(17)30591-0. doi:, 10.1016/j.jmb.2017.12.010. PMID:29277294<ref>PMID:29277294</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6ana" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Julien, J P]]
		+	[[Category: Orbea, J Ereno]]
		+	[[Category: Sicard, T]]
		+	[[Category: Antibody]]
		+	[[Category: Cd22]]
		+	[[Category: Fab]]
		+	[[Category: Immune system]]
		+	[[Category: Vhh domain]]

OCA at 07:08, 10 January 2018

OCA — Wed, 10 Jan 2018 07:08:31 GMT

←Older revision		Revision as of 07:08, 10 January 2018
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ana is ON HOLD ~~until Paper Publication~~	+	The entry 6ana is ON HOLD

	Authors:		Authors:

OCA at 06:29, 22 November 2017

OCA — Wed, 22 Nov 2017 06:29:48 GMT

←Older revision		Revision as of 06:29, 22 November 2017
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6ana is ON HOLD	+	The entry 6ana is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6ana" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:35:18 GMT

Protected "6ana" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:35, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6ana is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 16:35:17 GMT

New page: '''Unreleased structure''' The entry 6ana is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6ana is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]