6aoj - Revision history

OCA at 14:21, 4 October 2023

OCA — Wed, 04 Oct 2023 14:21:55 GMT

←Older revision		Revision as of 14:21, 4 October 2023
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	==Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal yeast Hog1p sequence==		==Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal yeast Hog1p sequence==
-	<StructureSection load='6aoj' size='340' side='right' caption='[[6aoj]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='6aoj' size='340' side='right'caption='[[6aoj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aoj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Legph Legph~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AOJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AOJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aoj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila_str._Philadelphia_1 Legionella pneumophila subsp. pneumophila str. Philadelphia 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AOJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AOJ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=CL:CHLORIDE+ION'>CL</scene~~>, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.902Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6aok\|6aok]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">lpg0096 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6aoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aoj OCA], [https://pdbe.org/6aoj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6aoj RCSB], [https://www.ebi.ac.uk/pdbsum/6aoj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6aoj ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6aoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aoj OCA], [~~http~~://pdbe.org/6aoj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6aoj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6aoj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6aoj ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q5ZZB5_LEGPH Q5ZZB5_LEGPH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Legph~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Cuff, M E~~]]	+	[[Category: Legionella pneumophila subsp. pneumophila str. Philadelphia 1]]
-	[[Category: ~~Egorova, O]]~~	+	[[Category: Cuff ME]]
-	~~[[Category: Evdokimova, E~~]]	+	[[Category: Di Leo R]]
-	[[Category: Leo, R Di]]	+	[[Category: Egorova O]]
-	[[Category: ~~Nocek, B~~]]	+	[[Category: Evdokimova E]]
-	[[Category: ~~Savchenko, A~~]]	+	[[Category: Nocek B]]
-	[[Category: ~~Stogios, P J~~]]	+	[[Category: Savchenko A]]
-	[[Category: ~~Yim, V~~]]	+	[[Category: Stogios PJ]]
-	[[Category: ~~Alpha/beta protein~~]]	+	[[Category: Yim V]]
-	[[Category: ~~Effector]]~~	+
-	~~[[Category: Had-like phosphatase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Infection]]~~	+
-	~~[[Category: Legionella pneumophila]]~~	+
-	~~[[Category: Phosphotyrosine~~]]	+

OCA at 08:21, 17 January 2018

OCA — Wed, 17 Jan 2018 08:21:25 GMT

←Older revision		Revision as of 08:21, 17 January 2018
Line 3:		Line 3:
	<StructureSection load='6aoj' size='340' side='right' caption='[[6aoj]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='6aoj' size='340' side='right' caption='[[6aoj]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6aoj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AOJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6aoj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Legph Legph]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AOJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6aok\|6aok]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6aok\|6aok]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">lpg0096 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272624 LEGPH])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aoj OCA], [http://pdbe.org/6aoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aoj RCSB], [http://www.ebi.ac.uk/pdbsum/6aoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aoj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aoj OCA], [http://pdbe.org/6aoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aoj RCSB], [http://www.ebi.ac.uk/pdbsum/6aoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aoj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Host colonization by Gram negative pathogens often involves delivery of bacterial proteins called ''effectors'' into the host cell. The pneumonia-causing pathogen Legionella pneumophila delivers more than 330 effectors into the host cell via its type IVB Dot/Icm secretion system. The collective functions of these proteins is the establishment of a replicative niche from which Legionella can recruit cellular materials to grow while evading lysosomal fusion inhibiting its growth. Using a combination of structural, biochemical, and in vivo approaches, we show that one of these translocated effector proteins, Ceg4, is a phosphotyrosine phosphatase harboring a haloacid dehalogenase hydrolase domain. Ceg4 could dephosphorylate a broad range of phosphotyrosine-containing peptides in vitro and attenuated activation of MAPK controlled pathways in both yeast and human cells. Our findings indicate that L. pneumophila's infectious program includes manipulation of phosphorylation cascades in key host pathways. The structural and functional features of the Ceg4 effector unraveled here, provide first insight into its function as a phosphotyrosine phosphatase, paving the way to further studies into L. pneumophila pathogenicity.
		+
		+	The Legionella pneumophila effector Ceg4 is a phosphotyrosine phosphatase that attenuates activation of eukaryotic MAPK pathways.,Quaile AT, Stogios PJ, Egorova O, Evdokimova E, Valleau D, Nocek B, Kompella PS, Peisajovich S, Yakunin AF, Ensminger AW, Savchenko A J Biol Chem. 2018 Jan 4. pii: M117.812727. doi: 10.1074/jbc.M117.812727. PMID:29301934<ref>PMID:29301934</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6aoj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Legph]]
	[[Category: Cuff, M E]]		[[Category: Cuff, M E]]
	[[Category: Egorova, O]]		[[Category: Egorova, O]]

OCA at 08:30, 10 January 2018

OCA — Wed, 10 Jan 2018 08:30:06 GMT

←Older revision		Revision as of 08:30, 10 January 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6aoj is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Legionella pneumophila effector Ceg4 with N-terminal yeast Hog1p sequence==
-		+	<StructureSection load='6aoj' size='340' side='right' caption='[[6aoj]], [[Resolution\|resolution]] 1.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6aoj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AOJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AOJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6aok\|6aok]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6aoj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6aoj OCA], [http://pdbe.org/6aoj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6aoj RCSB], [http://www.ebi.ac.uk/pdbsum/6aoj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6aoj ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Cuff, M E]]
		+	[[Category: Egorova, O]]
		+	[[Category: Evdokimova, E]]
		+	[[Category: Leo, R Di]]
		+	[[Category: Nocek, B]]
		+	[[Category: Savchenko, A]]
		+	[[Category: Stogios, P J]]
		+	[[Category: Yim, V]]
		+	[[Category: Alpha/beta protein]]
		+	[[Category: Effector]]
		+	[[Category: Had-like phosphatase]]
		+	[[Category: Hydrolase]]
		+	[[Category: Infection]]
		+	[[Category: Legionella pneumophila]]
		+	[[Category: Phosphotyrosine]]

OCA: Protected "6aoj" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:37:19 GMT

Protected "6aoj" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:37, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6aoj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 16:37:18 GMT

New page: '''Unreleased structure''' The entry 6aoj is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6aoj is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]