6apj - Revision history

OCA at 12:28, 17 July 2019

OCA — Wed, 17 Jul 2019 12:28:34 GMT

←Older revision		Revision as of 12:28, 17 July 2019
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	==Crystal Structure of human ST6GALNAC2==		==Crystal Structure of human ST6GALNAC2==
-	<StructureSection load='6apj' size='340' side='right' caption='[[6apj]], [[Resolution\|resolution]] 3.10Å' scene=''>	+	<StructureSection load='6apj' size='340' side='right'caption='[[6apj]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6apj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[6apj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APJ FirstGlance]. <br>
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	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Forouhar, F]]		[[Category: Forouhar, F]]
	[[Category: Moremen, K W]]		[[Category: Moremen, K W]]

OCA at 06:02, 3 January 2018

OCA — Wed, 03 Jan 2018 06:02:37 GMT

←Older revision		Revision as of 06:02, 3 January 2018
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	<StructureSection load='6apj' size='340' side='right' caption='[[6apj]], [[Resolution\|resolution]] 3.10Å' scene=''>		<StructureSection load='6apj' size='340' side='right' caption='[[6apj]], [[Resolution\|resolution]] 3.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6apj]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6apj]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APJ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ST6GALNAC2, SIAT7B, SIATL1, STHM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apj OCA], [http://pdbe.org/6apj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apj RCSB], [http://www.ebi.ac.uk/pdbsum/6apj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apj ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apj OCA], [http://pdbe.org/6apj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apj RCSB], [http://www.ebi.ac.uk/pdbsum/6apj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apj ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Vertebrate glycoproteins and glycolipids are synthesized in complex biosynthetic pathways localized predominantly within membrane compartments of the secretory pathway. The enzymes that catalyze these reactions are exquisitely specific, yet few have been extensively characterized because of challenges associated with their recombinant expression as functional products. We used a modular approach to create an expression vector library encoding all known human glycosyltransferases, glycoside hydrolases, and sulfotransferases, as well as other glycan-modifying enzymes. We then expressed the enzymes as secreted catalytic domain fusion proteins in mammalian and insect cell hosts, purified and characterized a subset of the enzymes, and determined the structure of one enzyme, the sialyltransferase ST6GalNAcII. Many enzymes were produced at high yields and at similar levels in both hosts, but individual protein expression levels varied widely. This expression vector library will be a transformative resource for recombinant enzyme production, broadly enabling structure-function studies and expanding applications of these enzymes in glycochemistry and glycobiology.
		+
		+	Expression system for structural and functional studies of human glycosylation enzymes.,Moremen KW, Ramiah A, Stuart M, Steel J, Meng L, Forouhar F, Moniz HA, Gahlay G, Gao Z, Chapla D, Wang S, Yang JY, Prabhakar PK, Johnson R, Rosa MD, Geisler C, Nairn AV, Seetharaman J, Wu SC, Tong L, Gilbert HJ, LaBaer J, Jarvis DL Nat Chem Biol. 2017 Dec 18. pii: nchembio.2539. doi: 10.1038/nchembio.2539. PMID:29251719<ref>PMID:29251719</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6apj" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Forouhar, F]]		[[Category: Forouhar, F]]
	[[Category: Moremen, K W]]		[[Category: Moremen, K W]]

OCA at 06:23, 20 December 2017

OCA — Wed, 20 Dec 2017 06:23:10 GMT

←Older revision		Revision as of 06:23, 20 December 2017
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-	'''Unreleased structure'''

-	~~The entry~~ 6apj ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of human ST6GALNAC2==
-		+	<StructureSection load='6apj' size='340' side='right' caption='[[6apj]], [[Resolution\|resolution]] 3.10Å' scene=''>
-	~~Authors~~: ~~Forouhar, F~~.~~, Moremen, K~~.W.~~, Tong, L~~.~~, Northeast Structural Genomics Consortium (NESG)~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6apj]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APJ FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of human ST6GALNAC2~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	[[~~Category~~: ~~Northeast Structural Genomics Consortium (Nesg)~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apj OCA], [http://pdbe.org/6apj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apj RCSB], [http://www.ebi.ac.uk/pdbsum/6apj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apj ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Moremen~~, K.W]]	+	</table>
		+	__TOC__
		+	</StructureSection>
	[[Category: Forouhar, F]]		[[Category: Forouhar, F]]
		+	[[Category: Moremen, K W]]
		+	[[Category: Structural genomic]]
	[[Category: Tong, L]]		[[Category: Tong, L]]
		+	[[Category: Glycosyltransferase]]
		+	[[Category: Nesg]]
		+	[[Category: Psi-biology]]
		+	[[Category: Transferase]]

OCA: Protected "6apj" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:39:03 GMT

Protected "6apj" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:39, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6apj is ON HOLD until Paper Publication Authors: Forouhar, F., Moremen, K.W., Tong, L., Northeast Structural Genomics Consortium (NESG) Description...

OCA — Wed, 27 Sep 2017 16:39:01 GMT

New page: '''Unreleased structure''' The entry 6apj is ON HOLD until Paper Publication Authors: Forouhar, F., Moremen, K.W., Tong, L., Northeast Structural Genomics Consortium (NESG) Description...

New page

'''Unreleased structure'''

The entry 6apj is ON HOLD until Paper Publication

Authors: Forouhar, F., Moremen, K.W., Tong, L., Northeast Structural Genomics Consortium (NESG)

Description: Crystal Structure of human ST6GALNAC2
[[Category: Unreleased Structures]]
[[Category: Northeast Structural Genomics Consortium (Nesg)]]
[[Category: Moremen, K.W]]
[[Category: Forouhar, F]]
[[Category: Tong, L]]