6apu - Revision history

OCA at 14:22, 4 October 2023

OCA — Wed, 04 Oct 2023 14:22:44 GMT

←Older revision		Revision as of 14:22, 4 October 2023
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	<StructureSection load='6apu' size='340' side='right'caption='[[6apu]], [[Resolution\|resolution]] 1.84Å' scene=''>		<StructureSection load='6apu' size='340' side='right'caption='[[6apu]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6apu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6APU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6apu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Trypanosoma_brucei_brucei Trypanosoma brucei brucei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6APU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3L6:(2-{[2-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)ETHYL](3-AMINOPROPYL)AMINO}ETHYL)PHOSPHONIC+ACID'>3L6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.842Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3L6:(2-{[2-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)ETHYL](3-AMINOPROPYL)AMINO}ETHYL)PHOSPHONIC+ACID'>3L6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6apu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apu OCA], [~~http~~://pdbe.org/6apu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6apu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6apu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6apu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6apu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apu OCA], [https://pdbe.org/6apu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6apu RCSB], [https://www.ebi.ac.uk/pdbsum/6apu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6apu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/HPRT_TRYBB HPRT_TRYBB]] Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).	+	[https://www.uniprot.org/uniprot/HPRT_TRYBB HPRT_TRYBB] Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Hypoxanthine phosphoribosyltransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Guddat, L~~]]	+	[[Category: Trypanosoma brucei brucei]]
-	[[Category: ~~Teran, D~~]]	+	[[Category: Guddat L]]
-	[[Category: ~~Acyclic nucleoside phosphonate]]~~	+	[[Category: Teran D]]
-	~~[[Category: Purine salvage]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 21:30, 28 October 2020

OCA — Wed, 28 Oct 2020 21:30:30 GMT

←Older revision		Revision as of 21:30, 28 October 2020
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	==Crystal structure of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltranferase in complex with (2-{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](3-aminopropyl)amino}ethyl)phosphonic acid==		==Crystal structure of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltranferase in complex with (2-{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](3-aminopropyl)amino}ethyl)phosphonic acid==
-	<StructureSection load='6apu' size='340' side='right' caption='[[6apu]], [[Resolution\|resolution]] 1.84Å' scene=''>	+	<StructureSection load='6apu' size='340' side='right'caption='[[6apu]], [[Resolution\|resolution]] 1.84Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6apu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6APU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6apu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6APU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3L6:(2-{[2-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)ETHYL](3-AMINOPROPYL)AMINO}ETHYL)PHOSPHONIC+ACID'>3L6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3L6:(2-{[2-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)ETHYL](3-AMINOPROPYL)AMINO}ETHYL)PHOSPHONIC+ACID'>3L6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6apu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apu OCA], [http://pdbe.org/6apu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apu RCSB], [http://www.ebi.ac.uk/pdbsum/6apu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6apu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apu OCA], [http://pdbe.org/6apu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apu RCSB], [http://www.ebi.ac.uk/pdbsum/6apu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 6apu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6apu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Phosphoribosyltransferase 3D structures\|Phosphoribosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 24:		Line 27:
	</StructureSection>		</StructureSection>
	[[Category: Hypoxanthine phosphoribosyltransferase]]		[[Category: Hypoxanthine phosphoribosyltransferase]]
		+	[[Category: Large Structures]]
	[[Category: Guddat, L]]		[[Category: Guddat, L]]
	[[Category: Teran, D]]		[[Category: Teran, D]]

OCA at 06:28, 14 March 2018

OCA — Wed, 14 Mar 2018 06:28:47 GMT

←Older revision		Revision as of 06:28, 14 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6apu is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltranferase in complex with (2-{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](3-aminopropyl)amino}ethyl)phosphonic acid==
		+	<StructureSection load='6apu' size='340' side='right' caption='[[6apu]], [[Resolution\|resolution]] 1.84Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6apu]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6APU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6APU FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3L6:(2-{[2-(2-AMINO-6-OXO-1,6-DIHYDRO-9H-PURIN-9-YL)ETHYL](3-AMINOPROPYL)AMINO}ETHYL)PHOSPHONIC+ACID'>3L6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Hypoxanthine_phosphoribosyltransferase Hypoxanthine phosphoribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.8 2.4.2.8] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6apu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6apu OCA], [http://pdbe.org/6apu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6apu RCSB], [http://www.ebi.ac.uk/pdbsum/6apu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6apu ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HPRT_TRYBB HPRT_TRYBB]] Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Due to toxicity and compliance issues and the emergence of resistance to current medications new drugs for the treatment of Human African Trypanosomiasis are needed. A potential approach to developing novel anti-trypanosomal drugs is by inhibition of the 6-oxopurine salvage pathways which synthesise the nucleoside monophosphates required for DNA/RNA production. This is in view of the fact that trypanosomes lack the machinery for de novo synthesis of the purine ring. To provide validation for this approach as a drug target, we have RNAi silenced the three 6-oxopurine phosphoribosyltransferase (PRTase) isoforms in the infectious stage of Trypanosoma brucei demonstrating that the combined activity of these enzymes is critical for the parasites' viability. Furthermore, we have determined crystal structures of two of these isoforms in complex with several acyclic nucleoside phosphonates (ANPs), a class of compound previously shown to inhibit 6-oxopurine PRTases from several species including Plasmodium falciparum. The most potent of these compounds have Ki values as low as 60 nM, and IC50 values in cell based assays as low as 4 muM. This data provides a solid platform for further investigations into the use of this pathway as a target for anti-trypanosomal drug discovery.

-	~~Authors:~~ Teran, D., ~~Guddat~~, L.	+	Evaluation of the Trypanosoma brucei 6-oxopurine salvage pathway as a potential target for drug discovery.,Dolezelova E, Teran D, Gahura O, Kotrbova Z, Prochazkova M, Keough D, Spacek P, Hockova D, Guddat L, Zikova A PLoS Negl Trop Dis. 2018 Feb 26;12(2):e0006301. doi:, 10.1371/journal.pntd.0006301. eCollection 2018 Feb. PMID:29481567<ref>PMID:29481567</ref>

-	~~Description: Crystal structure~~ of ~~Trypanosoma brucei hypoxanthine~~-~~guanine phosphoribosyltranferase in complex with (2~~-~~{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](3-aminopropyl)amino}ethyl)phosphonic acid~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
		+	<div class="pdbe-citations 6apu" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Hypoxanthine phosphoribosyltransferase]]
	[[Category: Guddat, L]]		[[Category: Guddat, L]]
	[[Category: Teran, D]]		[[Category: Teran, D]]
		+	[[Category: Acyclic nucleoside phosphonate]]
		+	[[Category: Purine salvage]]
		+	[[Category: Transferase]]

OCA: Protected "6apu" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:39:33 GMT

Protected "6apu" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:39, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6apu is ON HOLD until Paper Publication Authors: Teran, D., Guddat, L. Description: Crystal structure of Trypanosoma brucei hypoxanthine-guanine ph...

OCA — Wed, 27 Sep 2017 16:39:32 GMT

New page: '''Unreleased structure''' The entry 6apu is ON HOLD until Paper Publication Authors: Teran, D., Guddat, L. Description: Crystal structure of Trypanosoma brucei hypoxanthine-guanine ph...

New page

'''Unreleased structure'''

The entry 6apu is ON HOLD until Paper Publication

Authors: Teran, D., Guddat, L.

Description: Crystal structure of Trypanosoma brucei hypoxanthine-guanine phosphoribosyltranferase in complex with (2-{[2-(2-amino-6-oxo-1,6-dihydro-9H-purin-9-yl)ethyl](3-aminopropyl)amino}ethyl)phosphonic acid
[[Category: Unreleased Structures]]
[[Category: Guddat, L]]
[[Category: Teran, D]]