OCA at 08:19, 20 March 2019

OCA — Wed, 20 Mar 2019 08:19:38 GMT

←Older revision		Revision as of 08:19, 20 March 2019
Line 1:		Line 1:

	==Structure of a phage anti-CRISPR protein==		==Structure of a phage anti-CRISPR protein==
-	<StructureSection load='6arz' size='340' side='right' caption='[[6arz]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='6arz' size='340' side='right'caption='[[6arz]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6arz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ARZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ARZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6arz]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_phage_jbd5 Pseudomonas phage jbd5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ARZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ARZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">JBD5_034 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1223261 Pseudomonas phage JBD5])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6arz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6arz OCA], [http://pdbe.org/6arz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6arz RCSB], [http://www.ebi.ac.uk/pdbsum/6arz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6arz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6arz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6arz OCA], [http://pdbe.org/6arz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6arz RCSB], [http://www.ebi.ac.uk/pdbsum/6arz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6arz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CRISPR (clustered regularly interspaced short palindromic repeat)-Cas adaptive immune systems are prevalent defense mechanisms in bacteria and archaea. They provide sequence-specific detection and neutralization of foreign nucleic acids such as bacteriophages and plasmids. One mechanism by which phages and other mobile genetic elements are able to overcome the CRISPR-Cas system is through the expression of anti-CRISPR proteins. Over 20 different families of anti-CRISPR proteins have been described, each of which inhibits a particular type of CRISPR-Cas system. In this work, we determined the structure of type I-E anti-CRISPR protein AcrE1 by X-ray crystallography. We show that AcrE1 binds to the CRISPR-associated helicase/nuclease Cas3 and that the C-terminal region of the anti-CRISPR protein is important for its inhibitory activity. We further show that AcrE1 can convert the endogenous type I-E CRISPR system into a programmable transcriptional repressor.IMPORTANCE The CRISPR-Cas immune system provides bacteria with resistance to invasion by potentially harmful viruses, plasmids, and other foreign mobile genetic elements. This study presents the first structural and mechanistic insight into a phage-encoded protein that inactivates the type I-E CRISPR-Cas system in Pseudomonas aeruginosa The interaction of this anti-CRISPR protein with the CRISPR-associated helicase/nuclease proteins Cas3 shuts down the CRISPR-Cas system and protects phages carrying this gene from destruction. This interaction also allows the repurposing of the endogenous type I-E CRISPR system into a programmable transcriptional repressor, providing a new biotechnological tool for genetic studies of bacteria encoding this type I-E CRISPR-Cas system.
		+
		+	Disabling a Type I-E CRISPR-Cas Nuclease with a Bacteriophage-Encoded Anti-CRISPR Protein.,Pawluk A, Shah M, Mejdani M, Calmettes C, Moraes TF, Davidson AR, Maxwell KL MBio. 2017 Dec 12;8(6). pii: mBio.01751-17. doi: 10.1128/mBio.01751-17. PMID:29233895<ref>PMID:29233895</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6arz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pseudomonas phage jbd5]]
	[[Category: Calmettes, C]]		[[Category: Calmettes, C]]
	[[Category: Davidson, A R]]		[[Category: Davidson, A R]]

OCA at 07:24, 29 August 2018

OCA — Wed, 29 Aug 2018 07:24:33 GMT

←Older revision		Revision as of 07:24, 29 August 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6arz is ~~ON HOLD until Aug 23 2019~~	+	==Structure of a phage anti-CRISPR protein==
-		+	<StructureSection load='6arz' size='340' side='right' caption='[[6arz]], [[Resolution\|resolution]] 2.50Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6arz]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ARZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ARZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6arz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6arz OCA], [http://pdbe.org/6arz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6arz RCSB], [http://www.ebi.ac.uk/pdbsum/6arz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6arz ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Calmettes, C]]
		+	[[Category: Davidson, A R]]
		+	[[Category: Maxwell, K L]]
		+	[[Category: Moraes, T F]]
		+	[[Category: Pawluk, A]]
		+	[[Category: Shah, M]]
		+	[[Category: Prophage protein. anti-crispr]]
		+	[[Category: Viral protein]]

OCA: Protected "6arz" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:43:40 GMT

Protected "6arz" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:43, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6arz is ON HOLD until Aug 23 2019 Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 16:43:39 GMT

New page: '''Unreleased structure''' The entry 6arz is ON HOLD until Aug 23 2019 Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6arz is ON HOLD until Aug 23 2019

Authors:

Description:
[[Category: Unreleased Structures]]

6arz - Revision history

OCA at 08:19, 20 March 2019

OCA at 07:24, 29 August 2018

OCA: Protected "6arz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6arz is ON HOLD until Aug 23 2019 Authors: Description: Category: Unreleased Structures