6axk - Revision history

OCA at 07:04, 3 April 2024

OCA — Wed, 03 Apr 2024 07:04:37 GMT

←Older revision		Revision as of 07:04, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='6axk' size='340' side='right'caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6axk' size='340' side='right'caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6AXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Plasmodium_falciparum Plasmodium falciparum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AXK FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>~~, ~~<scene name='pdbligand=MES:~~2~~-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.103Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [~~http~~://pdbe.org/6axk PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6axk PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [https://pdbe.org/6axk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [https://www.ebi.ac.uk/pdbsum/6axk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/CSP_PLAFA CSP_PLAFA] The circumsporozoite protein is the immunodominant surface antigen on the sporozoite (the infective stage of the malaria parasite that is transmitted from the mosquito to the vertebrate host).
-	~~Acquired resistance against antimalarial drugs has further increased the need for an effective malaria vaccine~~. The ~~current leading candidate, RTS,S, is a recombinant~~ circumsporozoite protein (CSP)-based vaccine against Plasmodium falciparum that contains 19 NANP repeats followed by a thrombospondin repeat domain. Although RTS,S has undergone extensive clinical testing and has progressed through phase III clinical trials, continued efforts are underway to enhance its efficacy and duration of ~~protection. Here, we determined~~ that ~~two monoclonal antibodies (mAbs 311 and 317), isolated~~ from a recent controlled human malaria infection trial exploring a delayed fractional dose, inhibit parasite development in vivo by at least 97%. Crystal structures of antibody fragments (Fabs) 311 and 317 with an (NPNA)3 peptide illustrate their different binding modes. Notwithstanding, one and three of the ~~three NPNA repeats adopt similar well-defined type I beta-turns with Fab311 and Fab317, respectively. Furthermore,~~ to ~~explore antibody binding in~~ the ~~context of P. falciparum CSP, we used negative-stain electron microscopy on a recombinant shortened CSP (rsCSP~~) construct saturated with Fabs. Both complexes display a compact rsCSP with multiple Fabs bound, with the rsCSP-Fab311 complex forming a highly organized helical structure. Together, these structural insights may aid in the design of a next-generation malaria vaccine.	+

-	Structural basis for antibody recognition of the NANP repeats in Plasmodium falciparum circumsporozoite protein.,Oyen D, Torres JL, Wille-Reece U, Ockenhouse CF, Emerling D, Glanville J, Volkmuth W, Flores-Garcia Y, Zavala F, Ward AB, King CR, Wilson IA Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10438-E10445. doi:, 10.1073/pnas.1715812114. Epub 2017 Nov 14. PMID:29138320<ref>PMID:29138320</ref>	+	==See Also==
-		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6axk" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Oyen, D~~]]	+	[[Category: Plasmodium falciparum]]
-	[[Category: ~~Wilson, I A~~]]	+	[[Category: Oyen D]]
-	[[Category: ~~Fab fragment]]~~	+	[[Category: Wilson IA]]
-	~~[[Category: Immune system~~]]	+

OCA at 21:33, 28 October 2020

OCA — Wed, 28 Oct 2020 21:33:47 GMT

←Older revision		Revision as of 21:33, 28 October 2020
Line 1:		Line 1:

	==Crystal structure of Fab311 complex==		==Crystal structure of Fab311 complex==
-	<StructureSection load='6axk' size='340' side='right' caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='6axk' size='340' side='right'caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6AXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6AXK FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [http://pdbe.org/6axk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [http://www.ebi.ac.uk/pdbsum/6axk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [http://pdbe.org/6axk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [http://www.ebi.ac.uk/pdbsum/6axk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 22:		Line 22:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Oyen, D]]		[[Category: Oyen, D]]
	[[Category: Wilson, I A]]		[[Category: Wilson, I A]]
	[[Category: Fab fragment]]		[[Category: Fab fragment]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 07:59, 6 December 2017

OCA — Wed, 06 Dec 2017 07:59:20 GMT

←Older revision		Revision as of 07:59, 6 December 2017
Line 3:		Line 3:
	<StructureSection load='6axk' size='340' side='right' caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='6axk' size='340' side='right' caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AXK FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AXK FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [http://pdbe.org/6axk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [http://www.ebi.ac.uk/pdbsum/6axk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [http://pdbe.org/6axk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [http://www.ebi.ac.uk/pdbsum/6axk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Acquired resistance against antimalarial drugs has further increased the need for an effective malaria vaccine. The current leading candidate, RTS,S, is a recombinant circumsporozoite protein (CSP)-based vaccine against Plasmodium falciparum that contains 19 NANP repeats followed by a thrombospondin repeat domain. Although RTS,S has undergone extensive clinical testing and has progressed through phase III clinical trials, continued efforts are underway to enhance its efficacy and duration of protection. Here, we determined that two monoclonal antibodies (mAbs 311 and 317), isolated from a recent controlled human malaria infection trial exploring a delayed fractional dose, inhibit parasite development in vivo by at least 97%. Crystal structures of antibody fragments (Fabs) 311 and 317 with an (NPNA)3 peptide illustrate their different binding modes. Notwithstanding, one and three of the three NPNA repeats adopt similar well-defined type I beta-turns with Fab311 and Fab317, respectively. Furthermore, to explore antibody binding in the context of P. falciparum CSP, we used negative-stain electron microscopy on a recombinant shortened CSP (rsCSP) construct saturated with Fabs. Both complexes display a compact rsCSP with multiple Fabs bound, with the rsCSP-Fab311 complex forming a highly organized helical structure. Together, these structural insights may aid in the design of a next-generation malaria vaccine.
		+
		+	Structural basis for antibody recognition of the NANP repeats in Plasmodium falciparum circumsporozoite protein.,Oyen D, Torres JL, Wille-Reece U, Ockenhouse CF, Emerling D, Glanville J, Volkmuth W, Flores-Garcia Y, Zavala F, Ward AB, King CR, Wilson IA Proc Natl Acad Sci U S A. 2017 Nov 28;114(48):E10438-E10445. doi:, 10.1073/pnas.1715812114. Epub 2017 Nov 14. PMID:29138320<ref>PMID:29138320</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6axk" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Oyen, D]]		[[Category: Oyen, D]]
	[[Category: Wilson, I A]]		[[Category: Wilson, I A]]
	[[Category: Fab fragment]]		[[Category: Fab fragment]]
	[[Category: Immune system]]		[[Category: Immune system]]

OCA at 07:47, 22 November 2017

OCA — Wed, 22 Nov 2017 07:47:34 GMT

←Older revision		Revision as of 07:47, 22 November 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6axk is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of Fab311 complex==
-		+	<StructureSection load='6axk' size='340' side='right' caption='[[6axk]], [[Resolution\|resolution]] 2.10Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6axk]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AXK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AXK FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6axk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6axk OCA], [http://pdbe.org/6axk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6axk RCSB], [http://www.ebi.ac.uk/pdbsum/6axk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6axk ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Oyen, D]]
		+	[[Category: Wilson, I A]]
		+	[[Category: Fab fragment]]
		+	[[Category: Immune system]]

OCA at 05:43, 25 October 2017

OCA — Wed, 25 Oct 2017 05:43:43 GMT

←Older revision		Revision as of 05:43, 25 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6axk is ON HOLD	+	The entry 6axk is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6axk" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 16:55:49 GMT

Protected "6axk" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:55, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6axk is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 16:55:48 GMT

New page: '''Unreleased structure''' The entry 6axk is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6axk is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]