6b1b - Revision history

OCA at 14:30, 4 October 2023

OCA — Wed, 04 Oct 2023 14:30:20 GMT

←Older revision		Revision as of 14:30, 4 October 2023
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	<StructureSection load='6b1b' size='340' side='right'caption='[[6b1b]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='6b1b' size='340' side='right'caption='[[6b1b]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b1b]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecobd Ecobd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1B OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B1B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b1b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B1B FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat"~~><scene name~~=~~'pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.944Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[5um5\|5um5]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">ECBD_3674 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469008 ECOBD])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1b OCA], [https://pdbe.org/6b1b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b1b RCSB], [https://www.ebi.ac.uk/pdbsum/6b1b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1b ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1b OCA], [~~http~~://pdbe.org/6b1b PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b1b RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b1b PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1b ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A140NG21_ECOBD A0A140NG21_ECOBD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6b1b" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6b1b" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monooxygenase 3D structures\|Monooxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Ecobd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kandavelu, P]]	+	[[Category: Kandavelu P]]
-	[[Category: Rose~~, J P~~]]	+	[[Category: Rose JP]]
-	[[Category: Wang~~, B C~~]]	+	[[Category: Wang BC]]
-	[[Category: Yan, Y]]	+	[[Category: Yan Y]]
-	[[Category: Zhou, D]]	+	[[Category: Zhou D]]
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Oxidoreductase active site loop mutant 7 2]]~~	+
-	~~[[Category: Oxygenase component]]~~	+
-	~~[[Category: Protein engineering: 4-hydroxyphenylacetate 3-monooxygenase~~]]	+

OCA at 22:58, 5 June 2019

OCA — Wed, 05 Jun 2019 22:58:39 GMT

←Older revision		Revision as of 22:58, 5 June 2019
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	<StructureSection load='6b1b' size='340' side='right'caption='[[6b1b]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='6b1b' size='340' side='right'caption='[[6b1b]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b1b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1B FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b1b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecobd Ecobd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1B FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5um5\|5um5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5um5\|5um5]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECBD_3674 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=469008 ECOBD])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1b OCA], [http://pdbe.org/6b1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1b RCSB], [http://www.ebi.ac.uk/pdbsum/6b1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1b ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1b OCA], [http://pdbe.org/6b1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1b RCSB], [http://www.ebi.ac.uk/pdbsum/6b1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1b ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	4-Hydroxyphenylacetate 3-hydroxylase (EcHpaB) from Escherichia coli is capable of efficient ortho-hydroxylation of a wide range of phenolic compounds and demonstrates great potential for broad chemoenzymatic applications. To understand the structural and mechanistic basis of its catalytic versatility, we elucidated the crystal structure of EcHpaB by X-ray crystallography, which revealed a unique loop structure covering the active site. We further performed mutagenesis studies of this loop to probe its role in substrate specificity and catalytic activity. Our results not only showed the loop has great plasticity and strong tolerance towards extensive mutagenesis, but also suggested a flexible loop that enables the entrance and stable binding of substrates into the active site is the key factor to the enzyme catalytic versatility. These findings lay the groundwork for editing the loop sequence and structure for generation of EcHpaB mutants with improved performance for broader laboratory and industrial use.
		+
		+	Structural Insights into Catalytic Versatility of the Flavin-dependent Hydroxylase (HpaB) from Escherichia coli.,Shen X, Zhou D, Lin Y, Wang J, Gao S, Kandavelu P, Zhang H, Zhang R, Wang BC, Rose J, Yuan Q, Yan Y Sci Rep. 2019 May 8;9(1):7087. doi: 10.1038/s41598-019-43577-w. PMID:31068633<ref>PMID:31068633</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b1b" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecobd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Kandavelu, P]]		[[Category: Kandavelu, P]]

OCA at 06:42, 23 May 2019

OCA — Thu, 23 May 2019 06:42:29 GMT

←Older revision		Revision as of 06:42, 23 May 2019
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-	'''Unreleased structure'''

-	~~The entry 6b1b is ON HOLD until May 20 2020~~	+	==STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT XS6 (APO Enzyme)==
-		+	<StructureSection load='6b1b' size='340' side='right'caption='[[6b1b]], [[Resolution\|resolution]] 1.94Å' scene=''>
-	~~Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b1b]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B1B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B1B FirstGlance]. <br>
-	~~Description:~~ STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT XS6 (APO Enzyme)	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMO:TRIMETHYLAMINE+OXIDE'>TMO</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[5um5\|5um5]]</td></tr>
-	[[~~Category~~: ~~Rose~~, J.P]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b1b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b1b OCA], [http://pdbe.org/6b1b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b1b RCSB], [http://www.ebi.ac.uk/pdbsum/6b1b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b1b ProSAT]</span></td></tr>
		+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Kandavelu, P]]		[[Category: Kandavelu, P]]
		+	[[Category: Rose, J P]]
		+	[[Category: Wang, B C]]
		+	[[Category: Yan, Y]]
	[[Category: Zhou, D]]		[[Category: Zhou, D]]
-	[[Category: ~~Yan, Y~~]]	+	[[Category: Oxidoreductase]]
-	[[Category: ~~Wang, B.C~~]]	+	[[Category: Oxidoreductase active site loop mutant 7 2]]
		+	[[Category: Oxygenase component]]
		+	[[Category: Protein engineering: 4-hydroxyphenylacetate 3-monooxygenase]]

OCA at 05:19, 6 March 2019

OCA — Wed, 06 Mar 2019 05:19:44 GMT

←Older revision		Revision as of 05:19, 6 March 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b1b is ON HOLD until ~~Mar 17~~ 2020	+	The entry 6b1b is ON HOLD until May 20 2020

	Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.		Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.

-	Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT ~~7.2~~ (APO Enzyme)	+	Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT XS6 (APO Enzyme)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rose, J.P]]		[[Category: Rose, J.P]]

OCA at 06:21, 19 December 2018

OCA — Wed, 19 Dec 2018 06:21:52 GMT

←Older revision		Revision as of 06:21, 19 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b1b is ON HOLD until ~~Dec 18 2019~~	+	The entry 6b1b is ON HOLD until Mar 17 2020

	Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.		Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.
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	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rose, J.P]]		[[Category: Rose, J.P]]
		+	[[Category: Kandavelu, P]]
	[[Category: Zhou, D]]		[[Category: Zhou, D]]
	[[Category: Yan, Y]]		[[Category: Yan, Y]]
	[[Category: Wang, B.C]]		[[Category: Wang, B.C]]
-	[[Category: Kandavelu, P]]

OCA at 18:25, 2 December 2018

OCA — Sun, 02 Dec 2018 18:25:46 GMT

←Older revision		Revision as of 18:25, 2 December 2018
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b1b is ON HOLD until ~~Paper Publication~~	+	The entry 6b1b is ON HOLD until Dec 18 2019

	Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.		Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.
Line 8:		Line 8:
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
	[[Category: Rose, J.P]]		[[Category: Rose, J.P]]
-	[[Category: Kandavelu, P]]
	[[Category: Zhou, D]]		[[Category: Zhou, D]]
	[[Category: Yan, Y]]		[[Category: Yan, Y]]
	[[Category: Wang, B.C]]		[[Category: Wang, B.C]]
		+	[[Category: Kandavelu, P]]

OCA at 06:02, 6 December 2017

OCA — Wed, 06 Dec 2017 06:02:31 GMT

←Older revision		Revision as of 06:02, 6 December 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b1b is ON HOLD	+	The entry 6b1b is ON HOLD until Paper Publication

	Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.		Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.
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	Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT 7.2 (APO Enzyme)		Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT 7.2 (APO Enzyme)
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Wang, B.C]]
-	[[Category: Zhou, D]]
-	[[Category: Kandavelu, P]]
	[[Category: Rose, J.P]]		[[Category: Rose, J.P]]
		+	[[Category: Kandavelu, P]]
		+	[[Category: Zhou, D]]
	[[Category: Yan, Y]]		[[Category: Yan, Y]]
		+	[[Category: Wang, B.C]]

OCA: Protected "6b1b" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 17:03:26 GMT

Protected "6b1b" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:03, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6b1b is ON HOLD Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P. Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE ...

OCA — Wed, 27 Sep 2017 17:03:25 GMT

New page: '''Unreleased structure''' The entry 6b1b is ON HOLD Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P. Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE ...

New page

'''Unreleased structure'''

The entry 6b1b is ON HOLD

Authors: Zhou, D., Kandavelu, P., Wang, B.C., Yan, Y., Rose, J.P.

Description: STRUCTURE OF 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE (HPAB), OXYGENASE COMPONENT FROM ESCHERICHIA COLI MUTANT 7.2 (APO Enzyme)
[[Category: Unreleased Structures]]
[[Category: Wang, B.C]]
[[Category: Zhou, D]]
[[Category: Kandavelu, P]]
[[Category: Rose, J.P]]
[[Category: Yan, Y]]