6b21 - Revision history

OCA at 14:30, 4 October 2023

OCA — Wed, 04 Oct 2023 14:30:45 GMT

←Older revision		Revision as of 14:30, 4 October 2023
Line 3:		Line 3:
	<StructureSection load='6b21' size='340' side='right'caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='6b21' size='340' side='right'caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Ecoli Ecoli~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B21 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.45Å</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amtB, ybaG, b0451, JW0441 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [~~http~~://pdbe.org/6b21 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [https://pdbe.org/6b21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [https://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI]] Involved in the uptake of ammonia.	+	[https://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI] Involved in the uptake of ammonia.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 23:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ecoli~~]]	+	[[Category: Escherichia coli K-12]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boone~~, C D~~]]	+	[[Category: Boone CD]]
-	[[Category: Laganowsky, A]]	+	[[Category: Laganowsky A]]
-	~~[[Category: Ammonia channel]]~~	+
-	~~[[Category: Amtb]]~~	+
-	~~[[Category: Cardiolipin]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Transporter~~]]	+

OCA at 10:11, 1 January 2020

OCA — Wed, 01 Jan 2020 10:11:19 GMT

←Older revision		Revision as of 10:11, 1 January 2020
Line 1:		Line 1:

	==Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin==		==Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin==
-	<StructureSection load='6b21' size='340' side='right' caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>	+	<StructureSection load='6b21' size='340' side='right'caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
Line 24:		Line 24:
	</StructureSection>		</StructureSection>
	[[Category: Ecoli]]		[[Category: Ecoli]]
		+	[[Category: Large Structures]]
	[[Category: Boone, C D]]		[[Category: Boone, C D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky, A]]

OCA at 08:01, 21 March 2018

OCA — Wed, 21 Mar 2018 08:01:16 GMT

←Older revision		Revision as of 08:01, 21 March 2018
Line 10:		Line 10:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI]] Involved in the uptake of ammonia.		[[http://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI]] Involved in the uptake of ammonia.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane proteins interact with a myriad of lipid species in the biological membrane, leading to a bewildering number of possible protein-lipid assemblies. Despite this inherent complexity, the identification of specific protein-lipid interactions and the crucial role of lipids in the folding, structure, and function of membrane proteins is emerging from an increasing number of reports. Fundamental questions remain, however, regarding the ability of specific lipid binding events to membrane proteins to alter remote binding sites for lipids of a different type, a property referred to as allostery [Monod J, Wyman J, Changeux JP (1965) J Mol Biol 12:88-118]. Here, we use native mass spectrometry to determine the allosteric nature of heterogeneous lipid binding events to membrane proteins. We monitored individual lipid binding events to the ammonia channel (AmtB) from Escherichia coli, enabling determination of their equilibrium binding constants. We found that different lipid pairs display a range of allosteric modulation. In particular, the binding of phosphatidylethanolamine and cardiolipin-like molecules to AmtB exhibited the largest degree of allosteric modulation, inspiring us to determine the cocrystal structure of AmtB in this lipid environment. The 2.45-A resolution structure reveals a cardiolipin-like molecule bound to each subunit of the trimeric complex. Mutation of a single residue in AmtB abolishes the positive allosteric modulation observed for binding phosphatidylethanolamine and cardiolipin-like molecules. Our results demonstrate that specific lipid-protein interactions can act as allosteric modulators for the binding of different lipid types to integral membrane proteins.
		+
		+	Allostery revealed within lipid binding events to membrane proteins.,Patrick JW, Boone CD, Liu W, Conover GM, Liu Y, Cong X, Laganowsky A Proc Natl Acad Sci U S A. 2018 Mar 5. pii: 1719813115. doi:, 10.1073/pnas.1719813115. PMID:29507234<ref>PMID:29507234</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b21" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 07:00, 14 March 2018

OCA — Wed, 14 Mar 2018 07:00:58 GMT

←Older revision		Revision as of 07:00, 14 March 2018
Line 3:		Line 3:
	<StructureSection load='6b21' size='340' side='right' caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>		<StructureSection load='6b21' size='340' side='right' caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">amtB, ybaG, b0451, JW0441 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [http://pdbe.org/6b21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [http://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [http://pdbe.org/6b21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [http://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>
	</table>		</table>
Line 11:		Line 12:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Boone, C D]]		[[Category: Boone, C D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky, A]]

OCA at 05:06, 8 March 2018

OCA — Thu, 08 Mar 2018 05:06:47 GMT

←Older revision		Revision as of 05:06, 8 March 2018
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6b21 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin==
-		+	<StructureSection load='6b21' size='340' side='right' caption='[[6b21]], [[Resolution\|resolution]] 2.45Å' scene=''>
-	~~Authors~~: ~~Boone~~, C.D., ~~Laganowsky~~, A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b21]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B21 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B21 FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of AmtB from E~~. ~~coli bound to TopFluor cardiolipin~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=C9V:[(2R,5R,11R,14S,24E)-14-[(acetyloxy)methyl]-8-{[5-(3,5-dimethyl-1H-pyrrol-2-yl-kappaN)-5-(3,5-dimethyl-2H-pyrrol-2-ylidene-kappaN)pentanoyl]oxy}-5,11-dihydroxy-2-{[(9E)-octadec-9-enoyl]oxy}-5,11,16-trioxo-4,6,10,12,15-pentaoxa-5lambda~5~,11lambda~5~-diphosphatritriacont-24-en-1-yl+(9E)-octadec-9-enoatato](difluoro)boron'>C9V</scene></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b21 OCA], [http://pdbe.org/6b21 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b21 RCSB], [http://www.ebi.ac.uk/pdbsum/6b21 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b21 ProSAT]</span></td></tr>
-	[[Category: Boone, C.D]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AMTB_ECOLI AMTB_ECOLI]] Involved in the uptake of ammonia.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Boone, C D]]
	[[Category: Laganowsky, A]]		[[Category: Laganowsky, A]]
		+	[[Category: Ammonia channel]]
		+	[[Category: Amtb]]
		+	[[Category: Cardiolipin]]
		+	[[Category: Membrane protein]]
		+	[[Category: Transporter]]

OCA at 05:28, 11 October 2017

OCA — Wed, 11 Oct 2017 05:28:03 GMT

←Older revision		Revision as of 05:28, 11 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b21 is ON HOLD	+	The entry 6b21 is ON HOLD until Paper Publication

	Authors: Boone, C.D., Laganowsky, A.		Authors: Boone, C.D., Laganowsky, A.

OCA: Protected "6b21" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 17:04:48 GMT

Protected "6b21" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:04, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6b21 is ON HOLD Authors: Boone, C.D., Laganowsky, A. Description: Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin [[Category: ...

OCA — Wed, 27 Sep 2017 17:04:47 GMT

New page: '''Unreleased structure''' The entry 6b21 is ON HOLD Authors: Boone, C.D., Laganowsky, A. Description: Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin [[Category: ...

New page

'''Unreleased structure'''

The entry 6b21 is ON HOLD

Authors: Boone, C.D., Laganowsky, A.

Description: Crystal structure of AmtB from E. coli bound to TopFluor cardiolipin
[[Category: Unreleased Structures]]
[[Category: Boone, C.D]]
[[Category: Laganowsky, A]]