6b29 - Revision history

OCA at 14:19, 13 March 2024

OCA — Wed, 13 Mar 2024 14:19:40 GMT

←Older revision		Revision as of 14:19, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='6b29' size='340' side='right'caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='6b29' size='340' side='right'caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6B29 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B29 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~STAC3 (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b29 OCA], [~~http~~://pdbe.org/6b29 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6b29 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6b29 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6b29 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b29 OCA], [https://pdbe.org/6b29 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b29 RCSB], [https://www.ebi.ac.uk/pdbsum/6b29 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b29 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
-	[~~[http~~://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN]] Native American myopathy.	+	[https://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN] Native American myopathy.
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN]
-	Excitation-contraction (EC) coupling in skeletal muscle requires functional and mechanical coupling between L-type voltage-gated calcium channels (CaV1.1) and the ryanodine receptor (RyR1). Recently, STAC3 was identified as an essential protein for EC coupling and is part of a group of three proteins that can bind and modulate L-type voltage-gated calcium channels. Here, we report crystal structures of tandem-SH3 domains of different STAC isoforms up to 1.2-A resolution. These form a rigid interaction through a conserved interdomain interface. We identify the linker connecting transmembrane repeats II and III in two different CaV isoforms as a binding site for the SH3 domains and report a crystal structure of the complex with the STAC2 isoform. The interaction site includes the location for a disease variant in STAC3 that has been linked to Native American myopathy (NAM). Introducing the mutation does not cause misfolding of the SH3 domains, but abolishes the interaction. Disruption of the interaction via mutations in the II-III loop perturbs skeletal muscle EC coupling, but preserves the ability of STAC3 to slow down inactivation of CaV1.2.	+
-		+
-	~~Structural insights into binding of STAC proteins to voltage-gated calcium channels.,Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F Proc Natl Acad Sci U S A. 2017 Oct 23. pii~~: ~~201708852. doi:, 10.1073~~/~~pnas.1708852114. PMID:29078335<ref>PMID:29078335<~~/~~ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.S. ~~National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6b29" style="background-color:#fffaf0;"><~~/~~div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Petegem, F ~~Van~~]]	+	[[Category: Van Petegem F]]
-	[[Category: ~~Yuen, S M.~~Wong King]]	+	[[Category: Wong King Yuen SM]]
-	~~[[Category: Excitation-contraction coupling]]~~	+
-	~~[[Category: Ion channel adaptor protein]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 08:32, 8 January 2020

OCA — Wed, 08 Jan 2020 08:32:43 GMT

←Older revision		Revision as of 08:32, 8 January 2020
Line 1:		Line 1:

	==Crystal structure of the second SH3 domain of STAC3 (309-364)==		==Crystal structure of the second SH3 domain of STAC3 (309-364)==
-	<StructureSection load='6b29' size='340' side='right' caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>	+	<StructureSection load='6b29' size='340' side='right'caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B29 FirstGlance]. <br>		<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B29 FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Petegem, F Van]]		[[Category: Petegem, F Van]]
	[[Category: Yuen, S M.Wong King]]		[[Category: Yuen, S M.Wong King]]

OCA at 07:41, 8 November 2017

OCA — Wed, 08 Nov 2017 07:41:33 GMT

←Older revision		Revision as of 07:41, 8 November 2017
Line 3:		Line 3:
	<StructureSection load='6b29' size='340' side='right' caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>		<StructureSection load='6b29' size='340' side='right' caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B29 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B29 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b29 OCA], [http://pdbe.org/6b29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b29 RCSB], [http://www.ebi.ac.uk/pdbsum/6b29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b29 ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">STAC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b29 OCA], [http://pdbe.org/6b29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b29 RCSB], [http://www.ebi.ac.uk/pdbsum/6b29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b29 ProSAT]</span></td></tr>
	</table>		</table>
	== Disease ==		== Disease ==
	[[http://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN]] Native American myopathy.		[[http://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN]] Native American myopathy.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Excitation-contraction (EC) coupling in skeletal muscle requires functional and mechanical coupling between L-type voltage-gated calcium channels (CaV1.1) and the ryanodine receptor (RyR1). Recently, STAC3 was identified as an essential protein for EC coupling and is part of a group of three proteins that can bind and modulate L-type voltage-gated calcium channels. Here, we report crystal structures of tandem-SH3 domains of different STAC isoforms up to 1.2-A resolution. These form a rigid interaction through a conserved interdomain interface. We identify the linker connecting transmembrane repeats II and III in two different CaV isoforms as a binding site for the SH3 domains and report a crystal structure of the complex with the STAC2 isoform. The interaction site includes the location for a disease variant in STAC3 that has been linked to Native American myopathy (NAM). Introducing the mutation does not cause misfolding of the SH3 domains, but abolishes the interaction. Disruption of the interaction via mutations in the II-III loop perturbs skeletal muscle EC coupling, but preserves the ability of STAC3 to slow down inactivation of CaV1.2.
		+
		+	Structural insights into binding of STAC proteins to voltage-gated calcium channels.,Wong King Yuen SM, Campiglio M, Tung CC, Flucher BE, Van Petegem F Proc Natl Acad Sci U S A. 2017 Oct 23. pii: 201708852. doi:, 10.1073/pnas.1708852114. PMID:29078335<ref>PMID:29078335</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6b29" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Petegem, F Van]]		[[Category: Petegem, F Van]]
	[[Category: Yuen, S M.Wong King]]		[[Category: Yuen, S M.Wong King]]

OCA at 07:22, 25 October 2017

OCA — Wed, 25 Oct 2017 07:22:23 GMT

←Older revision		Revision as of 07:22, 25 October 2017
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6b29 is ~~ON HOLD~~ ~~until Paper Publication~~	+	==Crystal structure of the second SH3 domain of STAC3 (309-364)==
-		+	<StructureSection load='6b29' size='340' side='right' caption='[[6b29]], [[Resolution\|resolution]] 1.30Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6b29]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B29 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B29 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b29 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b29 OCA], [http://pdbe.org/6b29 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b29 RCSB], [http://www.ebi.ac.uk/pdbsum/6b29 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b29 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Disease ==
		+	[[http://www.uniprot.org/uniprot/STAC3_HUMAN STAC3_HUMAN]] Native American myopathy.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Petegem, F Van]]
		+	[[Category: Yuen, S M.Wong King]]
		+	[[Category: Excitation-contraction coupling]]
		+	[[Category: Ion channel adaptor protein]]
		+	[[Category: Protein binding]]

OCA at 05:28, 11 October 2017

OCA — Wed, 11 Oct 2017 05:28:18 GMT

←Older revision		Revision as of 05:28, 11 October 2017
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6b29 is ON HOLD	+	The entry 6b29 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6b29" [edit=sysop:move=sysop]

OCA — Wed, 27 Sep 2017 17:05:18 GMT

Protected "6b29" [edit=sysop:move=sysop]

←Older revision

Revision as of 17:05, 27 September 2017

OCA: New page: '''Unreleased structure''' The entry 6b29 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Sep 2017 17:05:17 GMT

New page: '''Unreleased structure''' The entry 6b29 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6b29 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]